ReadiCleave™ ROXtra AML-NHS ester
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1379.58 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | SDSProtocol |
Molecular weight 1379.58 |
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML ROXtra contains an azidomethyl linker that can be cleaved with TCEP to remove the ROXtra fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. ROXtra is the newly developed fluorophore that has the same spectra to the standard ROX dye with significantly improve stability and water solubility.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ ROXtra AML-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 72.486 µL | 362.429 µL | 724.858 µL | 3.624 mL | 7.249 mL |
5 mM | 14.497 µL | 72.486 µL | 144.972 µL | 724.858 µL | 1.45 mL |
10 mM | 7.249 µL | 36.243 µL | 72.486 µL | 362.429 µL | 724.858 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ReadiCleave™ AML Cy5 NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
ReadiCleave™ XFD532 AML-NHS ester | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
ReadiCleave™ FITC AML-NHS ester | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
ReadiCleave™ XFD488 AML-NHS ester | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
ReadiCleave™ XFD546 AML-NHS ester | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
ReadiCleave™ XFD647 AML-NHS ester | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
References
View all 50 references: Citation Explorer
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Authors: Chung, Yoo Na and Yoo, In Young and Yun, Sun Ae and Kim, Ji-Youn and Lee, Nam Yong and Huh, Hee Jae
Journal: Annals of laboratory medicine (2021): 506-509
Authors: Chung, Yoo Na and Yoo, In Young and Yun, Sun Ae and Kim, Ji-Youn and Lee, Nam Yong and Huh, Hee Jae
Journal: Annals of laboratory medicine (2021): 506-509
Development and application of SYBR Green Ⅰ real-time quantitative reverse transcription PCR assay for detection of swine Getah virus.
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Journal: Molecular and cellular probes (2021): 101730
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Journal: Gene (2021): 145606
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Journal: Clinica chimica acta; international journal of clinical chemistry (2021): 156-161
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Authors: Hess, Sandra and Niessner, Reinhard and Seidel, Michael
Journal: Journal of virological methods (2021): 114128
Authors: Hess, Sandra and Niessner, Reinhard and Seidel, Michael
Journal: Journal of virological methods (2021): 114128
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Journal: Water research (2021): 117104
Identification of the novel HLA-A*68:100 allele by PCR-SBT in a Chinese bone marrow donor.
Authors: Liu, Yuanyuan and Guo, Zhiqiang and Wu, Kangle and Tang, Zhaohui and Li, Guoying
Journal: HLA (2021): 539-540
Authors: Liu, Yuanyuan and Guo, Zhiqiang and Wu, Kangle and Tang, Zhaohui and Li, Guoying
Journal: HLA (2021): 539-540
Comparative evaluation of a dual-target real-time RT-PCR assay for COVID-19 diagnosis and assessment of performance in pooled saliva and nasopharyngeal swab samples.
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Journal: Expert review of molecular diagnostics (2021)
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Journal: Gene reports (2021): 101140
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Authors: van Grootveld, Rebecca and van Paassen, Judith and de Boer, Mark G J and Claas, Eric C J and Kuijper, Ed J and van der Beek, Martha T and ,
Journal: Mycoses (2021): 641-650