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AAT Bioquest

ReadiCleave™ ROXtra AML-NHS ester

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML ROXtra contains an azidomethyl linker that can be cleaved with TCEP to remove the ROXtra fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. ROXtra is the newly developed fluorophore that has the same spectra to the standard ROX dye with significantly improve stability and water solubility.

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ReadiCleave™ AML Cy5 NHS ester65167025000010.271, 0.420.020.03
ReadiCleave™ XFD532 AML-NHS ester534553810000.6110.240.09
ReadiCleave™ FITC AML-NHS ester4985178000010.79001, 0.9520.320.35
ReadiCleave™ XFD488 AML-NHS ester499520730000.9210.30.11
ReadiCleave™ XFD546 AML-NHS ester5615721120000.7910.210.12
ReadiCleave™ XFD647 AML-NHS ester6506712390000.3310.000.03

References

View all 50 references: Citation Explorer
Development of a PCR-lateral flow assay for rapid detection of Yersinia pestis, the causative agent of plague: PCR-LF assay for Yersinia pestis.
Authors: Singh, Rita and Pal, Vijai and Kumar, Manoj and Tripathi, N K and Goel, A K
Journal: Acta tropica (2021): 105958
Testing of four-sample pools offers resource optimization without compromising diagnostic performance of real time reverse transcriptase-PCR assay for COVID-19.
Authors: Singh, Anirudh K and Nema, Ram Kumar and Joshi, Ankur and Shankar, Prem and Gupta, Sudheer and Yadav, Ashvini Kumar and Nema, Shashwati and Mathew, Bijina J and Shrivas, Arti and Patankar, Chitra and Raghuwanshi, Arun and Pandey, Ritu and Tripathi, Ranu and Ansari, Kudsia and Singh, Kuldeep and Yadav, Jogender and Biswas, Debasis and Singh, Sarman
Journal: PloS one (2021): e0251891
Disruptors, a new class of oligonucleotide reagents, significantly improved PCR performance on templates containing stable intramolecular secondary structures.
Authors: Liu, Zhaocheng and Zhao, Chengxue and Zhao, Guodong and Xiong, Shangmin and Ma, Yong and Zheng, Minxue
Journal: Analytical biochemistry (2021): 114169
Laboratory Diagnosis of 37 Cases of Bartonella Endocarditis Based on Enzyme Immunoassay and Real-Time PCR.
Authors: Shapira, Lev and Rasis, Michal and Binsky Ehrenreich, Inbal and Maor, Yasmin and Katchman, Eugene A and Treves, Adi and Velan, Ariel and Halutz, Ora and Graidy-Varon, Merav and Leibovitch, Cecilia and Maisler, Noam and Ephros, Moshe and Giladi, Michael
Journal: Journal of clinical microbiology (2021)
Real-time PCR-based Y-specific sperm quantification assay in Queensland fruit fly: Insights to patterns of sperm storage.
Authors: Shadmany, J and Lee, S F and Taylor, P W
Journal: Insect molecular biology (2021): 315-324
Page updated on September 19, 2024

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Catalog Number7002
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Physical properties

Molecular weight

1379.58

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501