ReadiCleave™ ROXtra AML-NHS ester
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML ROXtra contains an azidomethyl linker that can be cleaved with TCEP to remove the ROXtra fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. ROXtra is the newly developed fluorophore that has the same spectra to the standard ROX dye with significantly improve stability and water solubility.
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ReadiCleave™ AML Cy5 NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
ReadiCleave™ XFD532 AML-NHS ester | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
ReadiCleave™ FITC AML-NHS ester | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
ReadiCleave™ XFD488 AML-NHS ester | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
ReadiCleave™ XFD546 AML-NHS ester | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
ReadiCleave™ XFD647 AML-NHS ester | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
References
View all 50 references: Citation Explorer
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Authors: Singh, Rita and Pal, Vijai and Kumar, Manoj and Tripathi, N K and Goel, A K
Journal: Acta tropica (2021): 105958
Testing of four-sample pools offers resource optimization without compromising diagnostic performance of real time reverse transcriptase-PCR assay for COVID-19.
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Journal: PloS one (2021): e0251891
Disruptors, a new class of oligonucleotide reagents, significantly improved PCR performance on templates containing stable intramolecular secondary structures.
Authors: Liu, Zhaocheng and Zhao, Chengxue and Zhao, Guodong and Xiong, Shangmin and Ma, Yong and Zheng, Minxue
Journal: Analytical biochemistry (2021): 114169
Authors: Liu, Zhaocheng and Zhao, Chengxue and Zhao, Guodong and Xiong, Shangmin and Ma, Yong and Zheng, Minxue
Journal: Analytical biochemistry (2021): 114169
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Journal: Journal of clinical microbiology (2021)
Authors: Shapira, Lev and Rasis, Michal and Binsky Ehrenreich, Inbal and Maor, Yasmin and Katchman, Eugene A and Treves, Adi and Velan, Ariel and Halutz, Ora and Graidy-Varon, Merav and Leibovitch, Cecilia and Maisler, Noam and Ephros, Moshe and Giladi, Michael
Journal: Journal of clinical microbiology (2021)
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Journal: Insect molecular biology (2021): 315-324
Authors: Shadmany, J and Lee, S F and Taylor, P W
Journal: Insect molecular biology (2021): 315-324
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