Amplite® Choline Quantitation Kit

Amplite® Choline Quantitation Kit is a fluorimetric assay kit for quantifying choline through a choline oxidase-mediated hydrogen peroxide readout in a microplate format.

Fluorimetric detection: Uses Amplite® Red to measure hydrogen peroxide generated by choline oxidase-mediated reactions.
High sensitivity: Detects choline as low as 100 nM (10 pmol/well) after 30 minutes.
Enzyme-coupled chemistry: Converts choline into a proportional hydrogen peroxide signal.
Flexible readout: Measure fluorescence at Ex/Em = 540/590 nm or absorbance at ~570 nm.
Applications: Suitable for choline quantitation in metabolic, membrane biology, and cholinergic research.

Choline dose response was obtained with Amplite® Choline Quantitation Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices). As low as 100 nM (10 picomole/well) of choline can be detected with 30 minutes incubation time (n=3).

Protocol

SDS

Catalog	Size	Price	Quantity
40007	200 Tests	Price

Overview

Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (acetylcholine synthesis). It is a major source for methyl groups via its metabolite, trimethylglycine that participates in the S-adenosylmethionine synthesis pathways. Choline deficiency may cause liver disease, atherosclerosis and possibly neurological disorders. Despite its importance in the central nervous system as a precursor for acetylcholine and membrane phosphatidylcholine, the role of choline in mental illness has been little studied. This Amplite® Choline Quantitation Kit provides one of the most sensitive methods for quantifying choline. The kit uses Amplite® Red to quantify the concentration of choline, which is related to the production of hydrogen peroxide in the choline oxidase-mediated enzyme coupling reactions. The amount of choline is proportional to the concentration of hydrogen peroxide formed in the enzyme coupling reaction cycle. In the presence of peroxidase, the fluorescence intensity of Amplite® Red is proportional to the formation of hydrogen peroxide that is converted to the concentration of choline. The assay can be readily read with a fluorescence microplate reader. Alternatively the assay can also be read at ~570 nm with an absorption microplate reader.

Citations

View all 7 citations: Citation Explorer

Novel insights into choline's role in promoting myofiber hypertrophy and mitochondrial function in muscle of sub-adult grass carp (Ctenopharyngodon idella)

Authors:

Li, Qingrui and Zhou, Xiaoqiu and Feng, Lin and Jiang, Weidan and Liu, Yang and Ma, Yaobin and Kuang, Shengyao and Li, Shuwei and Tang, Ling and Zhong, Chengbo and others,

Journal:

Animal Nutrition (2025)

The betA/B genes as a key factor in Cronobacter sakazakii survival under desiccation stress

Authors:

Yang, Y and Wang, W and Meng, X and Yang, J and Niu, B and Chen, Q

Journal:

Journal of Dairy Science (2025)

Structure and mechanism of the osmoregulated choline transporter BetT

Authors:

Yang, Tianjiao and Nian, Yuwei and Lin, Huajian and Li, Jing and Lin, Xiang and Li, Tianming and Wang, Ruiying and Wang, Longfei and Beattie, Gwyn A and Zhang, Jinru and others,

Journal:

Science Advances (2024): eado6229

Discovery of potent chromone-based autotaxin inhibitors inspired by cannabinoids

Authors:

Eymery, Mathias Christophe and Nguyen, Kim-Anh and Basu, Shibom and Hausmann, Jens and Nguyen, Viet-Khoa Tran and Seidel, Hans Peter and Gutierrez, Lola and Boumendjel, Ahc{\`e}ne and McCarthy, Andrew Aloysius

Journal:

European Journal of Medicinal Chemistry (2023): 115944

Branched-chain amino acids govern the high learning ability phenotype in Tokai high avoider (THA) rats

Authors:

Shida, Yukari and Endo, Hitoshi and Owada, Satoshi and Inagaki, Yutaka and Sumiyoshi, Hideaki and Kamiya, Akihide and Eto, Tomoo and Tatemichi, Masayuki

Journal:

Scientific reports (2021): 1--16

References

View all 62 references: Citation Explorer

Real-time multiplex PCR assay for genotyping of three apolipoprotein E alleles and two choline acetyltransferase alleles with three hybridization probes

Authors:

Park HD, Park KU, Kim KW, Song J, Chang HE, Heo SR, Lee HJ, Kim JQ.

Journal:

Clin Chem Lab Med (2007): 346

[Methyl-3H]-choline incorporation into MCF-7 cells: correlation with proliferation, choline kinase and phospholipase D assay

Authors:

Al-Saeedi F, Smith T, Welch A.

Journal:

Anticancer Res (2007): 901

Rapid high-throughput detection of peroxide with an acridinium-9-carboxamide: a homogeneous chemiluminescent assay for plasma choline

Authors:

Adamczyk M, Brashear RJ, Mattingly PG.

Journal:

Bioorg Med Chem Lett (2006): 2407

A simple and rapid radiochemical choline acetyltransferase (ChAT) assay screening test

Authors:

Shiba K, Ogawa K, Kinuya S, Yajima K, Mori H.

Journal:

J Neurosci Methods (2006): 98

Comparison of different sperm detection methods: amplification of fragment 3 of amelogenin gene (AMY 3), acid phosphatase detection, microcristalline choline assay and microscopic examination

Authors:

Rydzewska MJ, Janica J, Pepinski W, Skawronska M, Niemcunowicz-Janica A.

Journal:

Rocz Akad Med Bialymst (2000): 63

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Email	sales@aatbio.com
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Page updated on October 8, 2026

Component A: Amplite® Red	1 vial
Component B: Choline Probe	2 bottles (lyophilized powder)
Component C: Choline Standard	1 vial (2.8 mg)
Component D: Assay Buffer	1 bottle (25 mL)
Component E: DMSO	1 vial (100 µL)