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Amplite® Choline Quantitation Kit
Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (acetylcholine synthesis). It is a major source for methyl groups via its metabolite, trimethylglycine that participates in the S-adenosylmethionine synthesis pathways. Choline deficiency may cause liver disease, atherosclerosis and possibly neurological disorders. Despite its importance in the central nervous system as a precursor for acetylcholine and membrane phosphatidylcholine, the role of choline in mental illness has been little studied. This Amplite® Choline Quantitation Kit provides one of the most sensitive methods for quantifying choline. The kit uses Amplite® Red to quantify the concentration of choline, which is related to the production of hydrogen peroxide in the choline oxidase-mediated enzyme coupling reactions. The amount of choline is proportional to the concentration of hydrogen peroxide formed in the enzyme coupling reaction cycle. In the presence of peroxidase, the fluorescence intensity of Amplite® Red is proportional to the formation of hydrogen peroxide that is converted to the concentration of choline. The assay can be readily read with a fluorescence microplate reader. Alternatively the assay can also be read at ~570 nm with an absorption microplate reader.
Choline dose response was obtained with Amplite® Choline Quantitation Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices). As low as 100 nM (10 picomole/well) of choline can be detected with 30 minutes incubation time (n=3).
Choline dose response was obtained with Amplite® Choline Quantitation Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices). As low as 100 nM (10 picomole/well) of choline can be detected with 30 minutes incubation time (n=3).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
40007200 Tests
Price
 
Spectral properties
Excitation (nm)571
Emission (nm)584
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on September 23, 2025