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Annexin V, Recombinant

10 ug pure annexin V was loaded onto 10% NuPAGE.
10 ug pure annexin V was loaded onto 10% NuPAGE.
10 ug pure annexin V was loaded onto 10% NuPAGE.
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Physical properties
Molecular weight~36000
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

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CAS
136107-94-3
Molecular weight
~36000
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. They are found in all cells except bacteria. Annexin V forms a shield around negatively charged phospholipid molecules. The formation of an annexin V shield blocks the entry of phospholipids into coagulation (clotting) reactions. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe in the annexin V affinity assay to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death.

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Citations


View all 1 citations: Citation Explorer
Response of Mammalian cells to non-thermal intense narrowband pulsed electric fields
Authors: Katsuki, Sunao and Li, Yulan and Miyakawa, Daiki and Yamada, Ryo and Onishi, Nobuaki and Lim, Soowon
Journal: (2017): 1358--1361

References


View all 90 references: Citation Explorer
Annexin A5-functionalized bimodal lipid-based contrast agents for the detection of apoptosis
Authors: van Tilborg GA, Mulder WJ, Deckers N, Storm G, Reutelingsperger CP, Strijkers GJ, Nicolay K.
Journal: Bioconjug Chem (2006): 741
Annexin V/beta5 integrin interactions regulate apoptosis of growth plate chondrocytes
Authors: Wang W, Kirsch T.
Journal: J Biol Chem (2006): 30848
Myocyte apoptosis during acute myocardial infarction in rats is related to early sarcolemmal translocation of annexin A5 in border zone
Authors: Monceau V, Belikova Y, Kratassiouk G, Robidel E, Russo-Marie F, Charlemagne D.
Journal: Am J Physiol Heart Circ Physiol (2006): H965
Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924
In-vivo visualization of radiation-induced apoptosis using (125)I-annexin V
Authors: Watanabe H, Murata Y, Miura M, Hasegawa M, Kawamoto T, Shibuya H.
Journal: Nucl Med Commun (2006): 81
Mapping of treatment-induced apoptosis in normal structures: 99mTc-Hynic-rh-annexin V SPECT and CT image fusion
Authors: Kartachova MS, Valdes Olmos RA, Haas RL, Hoebers FJ, van den Brekel MW, van Z and wijk N, Herk M, Verheij M.
Journal: Eur J Nucl Med Mol Imaging (2006): 893
Non-fusion expression in Escherichia coli: Single-step purification of recombinant human annexin A5 for detection of apoptosis
Authors: Wang F, He XW, Yan HL, Huang JJ, Zhang Y, Jiang L, Gao YJ, Sun SH.
Journal: Protein Expr Purif (2006): 80
Quantum dots based probes conjugated to annexin V for photostable apoptosis detection and imaging
Authors: Le Gac S, Vermes I, van den Berg A.
Journal: Nano Lett (2006): 1863
Lymphoma cells protected from apoptosis by dysregulated bcl-2 continue to bind annexin V in response to B-cell receptor engagement: a cautionary tale
Authors: Holder MJ, Barnes NM, Gregory CD, Gordon J.
Journal: Leuk Res (2006): 77
The impact of altered annexin I protein levels on apoptosis and signal transduction pathways in prostate cancer cells
Authors: Hsiang CH, Tunoda T, Whang YE, Tyson DR, Ornstein DK.
Journal: Prostate (2006): 1413