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Annexin V, Recombinant

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. They are found in all cells except bacteria. Annexin V forms a shield around negatively charged phospholipid molecules. The formation of an annexin V shield blocks the entry of phospholipids into coagulation (clotting) reactions. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe in the annexin V affinity assay to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death.

Citations

View all 1 citations: Citation Explorer
Response of Mammalian cells to non-thermal intense narrowband pulsed electric fields
Authors: Katsuki, Sunao and Li, Yulan and Miyakawa, Daiki and Yamada, Ryo and Onishi, Nobuaki and Lim, Soowon
Journal: (2017): 1358--1361

References

View all 90 references: Citation Explorer
Quantum dots based probes conjugated to annexin V for photostable apoptosis detection and imaging
Authors: Le Gac S, Vermes I, van den Berg A.
Journal: Nano Lett (2006): 1863
PET imaging of apoptosis with (64)Cu-labeled streptavidin following pretargeting of phosphatidylserine with biotinylated annexin-V
Authors: Cauchon N, Langlois R, Rousseau JA, Tessier G, Cadorette J, Lecomte R, Hunting DJ, Pavan RA, Zeisler SK, van Lier JE.
Journal: Eur J Nucl Med Mol Imaging. (2006)
The impact of altered annexin I protein levels on apoptosis and signal transduction pathways in prostate cancer cells
Authors: Hsiang CH, Tunoda T, Whang YE, Tyson DR, Ornstein DK.
Journal: Prostate (2006): 1413
Lymphoma cells protected from apoptosis by dysregulated bcl-2 continue to bind annexin V in response to B-cell receptor engagement: a cautionary tale
Authors: Holder MJ, Barnes NM, Gregory CD, Gordon J.
Journal: Leuk Res (2006): 77
Non-fusion expression in Escherichia coli: Single-step purification of recombinant human annexin A5 for detection of apoptosis
Authors: Wang F, He XW, Yan HL, Huang JJ, Zhang Y, Jiang L, Gao YJ, Sun SH.
Journal: Protein Expr Purif (2006): 80
Page updated on September 8, 2024

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Unit size
100 ug
1 mg
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Physical properties

Molecular weight

~36000

Solvent

Water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

136107-94-3
10 ug pure annexin V was loaded onto 10% NuPAGE.
10 ug pure annexin V was loaded onto 10% NuPAGE.
10 ug pure annexin V was loaded onto 10% NuPAGE.