Biotin-PEG4-tyramide

Biotin-PEG4-tyramide is a reagent that has been used for tyramide signal amplification (TSA) via catalyzed reporter deposition (CARD). In CARD, a reporter enzyme, such as horseradish peroxidase (HRP) conjugated to a secondary antibody, is bound to the target of interest and catalyzes the covalent deposition of biotinyl tyramide to the sample. The sample is then probed by a detector, such as streptavidin-HRP, allowing detection via chromogenic or fluorescent methods. Biotin-PEG4-tyramide has been used in immunohistochemistry, ELISA, Western blot, and in situ hybridization applications. It tends to have improved signal/background than biotinyl tyramide in most of biological detections.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
45352	200 Slides	Price

References

View all 18 references: Citation Explorer

High specificity and tight spatial restriction of self-biotinylation by DNA and RNA G-Quadruplexes complexed in vitro and in vivo with Heme.

Authors:

Lat, Prince Kumar and Liu, Kun and Kumar, Dev N and Wong, Kenneth K L and Verheyen, Esther M and Sen, Dipankar

Journal:

Nucleic acids research (2020)

Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity.

Authors:

Li, Wei and Zeng, Weiwu and Chen, Yi and Wang, Fang and Wu, Fan and Weng, Xiaocheng and Zhou, Xiang

Journal:

The Analyst (2019): 4472-4476

APEX Peroxidase-Catalyzed Proximity Labeling and Multiplexed Quantitative Proteomics.

Authors:

Kalocsay, Marian

Journal:

Methods in molecular biology (Clifton, N.J.) (2019): 41-55

Proximity labeling of cis-ligands of CD22/Siglec-2 reveals stepwise α2,6 sialic acid-dependent and -independent interactions.

Authors:

Alborzian Deh Sheikh, Amin and Akatsu, Chizuru and Imamura, Akihiro and Abdu-Allah, Hajjaj H M and Takematsu, Hiromu and Ando, Hiromune and Ishida, Hideharu and Tsubata, Takeshi

Journal:

Biochemical and biophysical research communications (2018): 854-859

Tyramide Signal Amplification Permits Immunohistochemical Analyses of Androgen Receptors in the Rat Prefrontal Cortex.

Authors:

Low, Katelyn L and Ma, Chunqi and Soma, Kiran K

Journal:

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2017): 295-308

Physical properties

Molecular weight	610.77
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025