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Chemical structure for Biotin-PEG4-tyramide.
Chemical structure for Biotin-PEG4-tyramide.
Ordering information
Price ()
Catalog Number45352
Unit Size
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Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight610.77
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
Biotin-PEG4-tyramide is a reagent that has been used for tyramide signal amplification (TSA) via catalyzed reporter deposition (CARD). In CARD, a reporter enzyme, such as horseradish peroxidase (HRP) conjugated to a secondary antibody, is bound to the target of interest and catalyzes the covalent deposition of biotinyl tyramide to the sample. The sample is then probed by a detector, such as streptavidin-HRP, allowing detection via chromogenic or fluorescent methods. Biotin-PEG4-tyramide has been used in immunohistochemistry, ELISA, Western blot, and in situ hybridization applications. It tends to have improved signal/background than biotinyl tyramide in most of biological detections.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin-PEG4-tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM163.728 µL818.639 µL1.637 mL8.186 mL16.373 mL
5 mM32.746 µL163.728 µL327.456 µL1.637 mL3.275 mL
10 mM16.373 µL81.864 µL163.728 µL818.639 µL1.637 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


View all 18 references: Citation Explorer
High specificity and tight spatial restriction of self-biotinylation by DNA and RNA G-Quadruplexes complexed in vitro and in vivo with Heme.
Authors: Lat, Prince Kumar and Liu, Kun and Kumar, Dev N and Wong, Kenneth K L and Verheyen, Esther M and Sen, Dipankar
Journal: Nucleic acids research (2020)
Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity.
Authors: Li, Wei and Zeng, Weiwu and Chen, Yi and Wang, Fang and Wu, Fan and Weng, Xiaocheng and Zhou, Xiang
Journal: The Analyst (2019): 4472-4476
APEX Peroxidase-Catalyzed Proximity Labeling and Multiplexed Quantitative Proteomics.
Authors: Kalocsay, Marian
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 41-55
Proximity labeling of cis-ligands of CD22/Siglec-2 reveals stepwise α2,6 sialic acid-dependent and -independent interactions.
Authors: Alborzian Deh Sheikh, Amin and Akatsu, Chizuru and Imamura, Akihiro and Abdu-Allah, Hajjaj H M and Takematsu, Hiromu and Ando, Hiromune and Ishida, Hideharu and Tsubata, Takeshi
Journal: Biochemical and biophysical research communications (2018): 854-859
Tyramide Signal Amplification Permits Immunohistochemical Analyses of Androgen Receptors in the Rat Prefrontal Cortex.
Authors: Low, Katelyn L and Ma, Chunqi and Soma, Kiran K
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2017): 295-308
Identification of Siglec Ligands Using a Proximity Labeling Method.
Authors: Chang, Lanyi and Chen, Yi-Ju and Fan, Chan-Yo and Tang, Chin-Ju and Chen, Yi-Hsiu and Low, Penk-Yeir and Ventura, Albert and Lin, Chun-Cheng and Chen, Yu-Ju and Angata, Takashi
Journal: Journal of proteome research (2017): 3929-3941
Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis.
Authors: Royston, Daniel J and Clasper, Steven and Jackson, David G
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 35-48
Development of a highly sensitive immunohistochemical method to detect neurochemical molecules in formalin-fixed and paraffin-embedded tissues from autopsied human brains.
Authors: Goto, Satoshi and Morigaki, Ryoma and Okita, Shinya and Nagahiro, Shinji and Kaji, Ryuji
Journal: Frontiers in neuroanatomy (2015): 22
Development of a sensitive ELISA for quantification of three- and four-repeat tau isoforms in tauopathies.
Authors: Luk, Connie and Giovannoni, Gavin and Williams, David R and Lees, Andrew J and de Silva, Rohan
Journal: Journal of neuroscience methods (2009): 34-42
Levels of pregnancy-associated plasma protein A in patients with coronary artery disease.
Authors: Liu, Zhi-Yuan and Zhang, Jin-Ying and Sun, Tong-Wen and Zhang, Yan-Jun and Zhang, Li and Wang, Le-Xin
Journal: Clinical and investigative medicine. Medecine clinique et experimentale (2008): E85-9