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Cell Meter™ Colorimetric WST-8 Cell Quantification Kit
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. Cell Meter™ Colorimetric WST-8 Cell Quantification Kit uses water-soluble WST-8 tetrazolium salt to quantify the number of live cells. The water-soluble WST-8 tetrazolium salt produces a water-soluble orange formazan dye upon bioreduction in the presence of an electron carrier, 1-methoxy-5-methylphenazinium methyl sulfate. The kit is convenient and robust with a mix and read format. WST-8 solution is added directly to the test cells, no pre-mixing of components is required. WST-8 tetrazolium salt is reduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells by monitoring absorbance increase at 460 nm. The excellent stability and little cytotoxicity of WST-8 solution make the kit useful for the assays that require long incubation (such as 24 to 48 hours). Cell Meter™ Colorimetric WST-8 Cell Quantification Kit provides a sensitive colorimetric assay for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salt-based assays such as MTT, XTT or MTS etc.
Cell number was determined with Cell Meter™ Colorimetric WST-8 Cell Quantification Kit. HeLa cells at 40 to 10,000 cells/well/100 µL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a SpectraMax reader (Molecular Devices).
Cell number was determined with Cell Meter™ Colorimetric WST-8 Cell Quantification Kit. HeLa cells at 40 to 10,000 cells/well/100 µL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a SpectraMax reader (Molecular Devices).
protocol
Protocol
COO
COA
CatalogSize
Price
Quantity
227701000 Tests
Price
227715000 Tests
Price
 
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Absorbance microplate reader
Absorbance460 nm
Recommended plateClear bottom
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Page updated on September 25, 2025