Cell Meter™ Colorimetric WST-8 Cell Quantification Kit
![Cell number was determined with Cell Meter™ Colorimetric WST-8 Cell Quantification Kit. HeLa cells at 40 to 10,000 cells/well/100 µL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a SpectraMax reader (Molecular Devices).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-meter-colorimetric-wst-8-cell-quantification-kit%2Ffigure-for-cell-meter-colorimetric-wst-8-cell-quantification-kit_Ix3Os.png&w=640&q=75)
![Cell number was determined with Cell Meter™ Colorimetric WST-8 Cell Quantification Kit. HeLa cells at 40 to 10,000 cells/well/100 µL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a SpectraMax reader (Molecular Devices).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-meter-colorimetric-wst-8-cell-quantification-kit%2Ffigure-for-cell-meter-colorimetric-wst-8-cell-quantification-kit_Ix3Os.png&w=640&q=75)
![Cell number was determined with Cell Meter™ Colorimetric WST-8 Cell Quantification Kit. HeLa cells at 40 to 10,000 cells/well/100 µL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a SpectraMax reader (Molecular Devices).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-meter-colorimetric-wst-8-cell-quantification-kit%2Ffigure-for-cell-meter-colorimetric-wst-8-cell-quantification-kit_Ix3Os.png&w=128&q=25)
![Cytotoxicity tests of HeLa cells in response to (A) SDS and (B) Staurosporine treatment were measured with Cell Meter™ Colorimetric WST-8 Cell Quantification Kit. HeLa cells at 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. Cells were treated with serially diluted SDS for 2 hours or Staurosporine for 4 hours. The absorbance was measured at 460 nm using a SpectraMax reader. ](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-meter-colorimetric-wst-8-cell-quantification-kit%2Ffigure-for-cell-meter-colorimetric-wst-8-cell-quantification-kit_MxVMg.jpg&w=128&q=25)
![Cell number was determined with Cell Meter™ Colorimetric WST-8 Cell Quantification Kit<strong>.</strong> Jurkat cells at 60 to 150,000 cells/well/100 µL were added in a clear bottom 96-well plate. The absorbance was measured at 460 nm using a SpectraMax reader (Molecular Devices).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-meter-colorimetric-wst-8-cell-quantification-kit%2Ffigure-for-cell-meter-colorimetric-wst-8-cell-quantification-kit_SK81h.png&w=128&q=25)
![FGF10 increased the viability of primary mouse corneal epithelial cells. Mouse corneal epithelial cells were seeded in multi-well dishes, treated with FGF10 (30 ng/mL) or BSA (control), and cultured for 1, 3, and 7 days. Cells were processed for viability assay (tetrazolium salt, WST- 8). Dots represent the number of repeats (n = 9) for each condition/time. Statistical significance was assessed with unpaired t-tests. All bar plots are mean ± SD. *P < 0.05; ****P < 0.0001. Source: <b>Role of FGF10/FGFR2b Signaling in Homeostasis and Regeneration of Adult Lacrimal Gland and Corneal Epithelium Proliferation</b> by Findburgh <em>et.al.</em>, <em>Investigative Ophthalmology & Visual Science</em> Jan. 2023.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcell-meter-colorimetric-wst-8-cell-quantification-kit%2Ffigure-for-cell-meter-colorimetric-wst-8-cell-quantification-kit_CLLfJ.png&w=96&q=25)
AT A GLANCE
Prepare cells in a 96-well plate (100 µL/well).
Add 10 µL of WST-8™ Solution to each well.
Incubate at 37°C for 1 - 4 hours.
Monitor absorbance at OD = 460 nm.
The WST-8™ Solution can be stable for up to one year if stored at 4°C and protected from light. Store it at <-20°C for longer storage.
CELL PREPARATION
For guidelines on cell sample preparation, please visit:
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
- Plate 5000 to 10,000 cells/well in a tissue culture microplate with clear bottom.
Add test compounds into the cells and incubate for a desired period of time (such as 24, 48, or 96 hours) in a 37°C, 5% CO2 incubator. For blank wells (medium without the cells), add the same amount of test compounds. The suggested total volume is 100 µL for a 96-well plate or 50 µL for a 384-well plate.
Note: Each cell line should be evaluated on an individual basis to determine the optimal cell density for proliferation or cytotoxicity induction. For proliferation assays, use fewer cells; for cytotoxicity assays, use more cells to start with.
Add 10 µL/well (96-well plate) or 5 µL/well (384-well plate) of WST-8™ Solution to each well.
Incubate the plate at 37°C for 1 - 4 hours, protect from light.
Note: The incubation time could be from 30 minutes to overnight depending on the individual cell type and cell concentration used. Optimize the incubation time for each experiment.
- Monitor the absorbance increase with an absorbance plate reader at OD =460 nm.
Prepare cell culture in a tissue culture microplate with a clear bottom. The suggested total volume is 100 µL for a 96-well plate or 50 µL for a 384-well plate.
Note: We used serially diluted HeLa and Jurkat cell suspension in a clear bottom 96-well plate for the assay.
Add 10 µL/well (96-well plate) or 5 µL/well (384-well plate) of WST-8™ Solution to each well.
Incubate the plate at 37°C for 1 - 4 hours, protect from light.
Note: The incubation time could be from 30 minutes to overnight depending on the individual cell type and cell concentration used. Optimize the incubation time for each experiment.
- Monitor the absorbance increase with an absorbance plate reader at OD = 460 nm.
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