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Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit
Deep Red Fluorescence
Cell Meter™ TUNEL apoptosis assay kit provides a robust tool for conveniently detecting apoptosis caused by DNA fragmentation. The assay is non-radioactive and rapid. The TUNEL assay uses the terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of TF5-dUTP at the free 3’-hydroxyl ends of the fragmented DNAs. The resulted TF5-labeled DNAs are analyzed by fluorescence microscopy (Cy5 filter set) or flow cytometry with 633 or 640 nm laser with 660/20 nm filter (APC channel). Its red emission can be conveniently multiplexed with GFP labelled targets. Direct incorporation of fluorescent TF5-labeled nucleotides significantly reduces the number of test steps. The kit is optimized to detect apoptosis in fixed cells and formalin-fixed, paraffin-embedded tissue sections.
<strong>Fluorescence&nbsp;</strong><strong>images of TUNEL assay&nbsp;</strong><strong>with&nbsp;&nbsp;</strong><strong>HeLa cells.&nbsp;</strong>&nbsp;&nbsp;<br />HeLa cells were fixed and treated with or without DNAse for 60 mins at 37 &deg;C. The cells were then stained with Cell Meter&trade; Fixed Cell and Tissue TUNEL Apoptosis Assay Kit (Cat#22855). DNA strand breaks showed intense fluorescent staining in DNAse treated cells. The signal was acquired with fluorescence microscope using a Cy5 filter set.<br>&nbsp;
<strong>Fluorescence&nbsp;</strong><strong>images of TUNEL assay&nbsp;</strong><strong>with&nbsp;&nbsp;</strong><strong>HeLa cells.&nbsp;</strong>&nbsp;&nbsp;<br />HeLa cells were fixed and treated with or without DNAse for 60 mins at 37 &deg;C. The cells were then stained with Cell Meter&trade; Fixed Cell and Tissue TUNEL Apoptosis Assay Kit (Cat#22855). DNA strand breaks showed intense fluorescent staining in DNAse treated cells. The signal was acquired with fluorescence microscope using a Cy5 filter set.<br>&nbsp;
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
2285525 Tests
Price
 
Spectral properties
Correction factor (280 nm)0.027
Extinction coefficient (cm -1 M -1)
250000
Excitation (nm)649
Emission (nm)663
Quantum yield
0.27
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Flow cytometer
Excitation633/640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel
Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 8, 2024