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Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit
Red Fluorescence Optimized for Flow Cytometry
Cell cycles have four sequential phases: G0/G1, S, G2, and M. During a cell's passage through cell cycle, its DNA is duplicated in S (synthesis) phase and distributed equally between two daughter cells in M (mitosis) phase. These two phases are separated by two gap phases: G0/G1 and G2. The two gap phases provide time for cells to grow and double the mass of their proteins and organelles. They are also used by cells to monitor internal and external conditions before proceeding with the next phase of cell cycle. The cell's passage through cell cycles is controlled by a host of different regulatory proteins. This assay kit is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Red™ CCS2 in live cells. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be determined by flow cytometry. Cells stained with Nuclear Red™ CCS2 can be monitored with a flow cytometer (PE-Texas Red channel).
<p>DNA profile in growing Jurkat cells. Jurkat cells were stained with Nuclear Red&trade; CCS2 for 30 minutes. The fluorescence intensity of Nuclear Red&trade; CCS2 was measured using ACEA NovoCyte flow cytometer with the channel of PE-Texas Red, the image was generated using Cell Cycle Analysis module of NovoExpress software. In growing Jurkat cells, G0/G1 and G2/M phase histogram peaks are separated by a S-phase distribution.</p>
<p>DNA profile in growing Jurkat cells. Jurkat cells were stained with Nuclear Red&trade; CCS2 for 30 minutes. The fluorescence intensity of Nuclear Red&trade; CCS2 was measured using ACEA NovoCyte flow cytometer with the channel of PE-Texas Red, the image was generated using Cell Cycle Analysis module of NovoExpress software. In growing Jurkat cells, G0/G1 and G2/M phase histogram peaks are separated by a S-phase distribution.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22860100 tests
Price
 
Spectral properties
Excitation (nm)542
Emission (nm)580
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission610/20 nm filter
Instrument specification(s)PE-Texas Red channel
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Page updated on September 25, 2025