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Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit
Optimized for 405 nm Violet Laser Excitation
The cell cycle has four sequential phases: G0/G1, S, G2, and M. During a cell's passage through cell cycle, its DNA is duplicated in S (synthesis) phase and distributed equally between two daughter cells in M (mitosis) phase. These two phases are separated by two gap phases: G0/G1 and G2. The two gap phases provide time for the cell to grow and double the mass of their proteins and organelles. They are also used by the cells to monitor internal and external conditions before proceeding with the next phase of cell cycle. The cell's passage through cell cycle is controlled by a host of different regulatory proteins. This particular kit is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Violet™ in live cells. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be determined by flow cytometry. Cells stained with Nuclear Violet™ can be monitored with a flow cytometer (Pacific Blue® channel).
DNA profile in growing Jurkat cells with Cell Meter™ Fluorimetric Cell Cycle Assay Kit. Jurkat cells were stained with Nuclear Violet™ for 30 minutes. The fluorescence intensity of Nuclear Violet™ was measured using ACEA NovoCyte flow cytometer with the channel of Pacific Blue. In growing Jurkat cells, G0/G1 and G2/M phase histogram peaks are separated by the S-phase distribution.
DNA profile in growing Jurkat cells with Cell Meter™ Fluorimetric Cell Cycle Assay Kit. Jurkat cells were stained with Nuclear Violet™ for 30 minutes. The fluorescence intensity of Nuclear Violet™ was measured using ACEA NovoCyte flow cytometer with the channel of Pacific Blue. In growing Jurkat cells, G0/G1 and G2/M phase histogram peaks are separated by the S-phase distribution.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22845100 Tests
Price
 
Spectral properties
Excitation (nm)401
Emission (nm)460
Storage, safety and handling
Intended useResearch Use Only (RUO)
Instrument settings
Flow cytometer
Excitation405 nm laser
Emission450/40 nm filter
Instrument specification(s)Pacific Blue channel
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Page updated on October 13, 2025