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Cell Navigator® Fluorimetric Lipid Droplet Assay Kit
Green Fluorescence
Lipid droplets, also referred to as lipid bodies, oil bodies or adiposomes, are lipid-rich cellular organelles that regulate the storage and hydrolysis of neutral lipids. They also serve as a reservoir of lipid source for many important biological processes such as fatty acid and cellular cholesterol for energy and membrane formation and maintenance. Abnormal accumulation of the cytoplasmic lipid droplets occurs in a variety of pathological conditions and can be an indicator of metabolic deficiency or pathogenesis. AAT Bioquest's Cell Navigator® Fluorimetric Lipid Droplets Assay Kit is a simple assay that could quantitatively measure lipid droplet accumulation. Nile Green™ is used in the kit for lipophilic stain. Nile Green™ is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. It is an excellent vital stain for the detection of intracellular lipid droplets with fluorescence microscopy, flow cytometry or fluorescence microplate reader. Unlike Nile Red which has broad range of fluorescence spectrum, Nile Green™ stains intracellular lipid droplets with green fluorescence only. It can be used with other fluorescence dyes for multicolor staining. The green fluorescence signal could be observed using the filter set of FITC.
Fluorescence images of intracellular lipid droplets in control (Left) and Oleic Acid treated HeLa cells (Right) using Cell Navigator® Lipid Droplets Fluorescence Assay Kit. HeLa cells were incubated with 300 uM of Oleic Acid for 24 hours to induce intracellular lipid droplets formation. After washing with PBS, the cells were labeled with 1X Nile Green™ and Hoechst 33342  (Cat#17533).
Fluorescence images of intracellular lipid droplets in control (Left) and Oleic Acid treated HeLa cells (Right) using Cell Navigator® Lipid Droplets Fluorescence Assay Kit. HeLa cells were incubated with 300 uM of Oleic Acid for 24 hours to induce intracellular lipid droplets formation. After washing with PBS, the cells were labeled with 1X Nile Green™ and Hoechst 33342  (Cat#17533).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22730200 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.015
Correction factor (280 nm)0.018
Extinction coefficient (cm -1 M -1)
81000
Excitation (nm)504
Emission (nm)510
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation485 nm
Emission520 nm
Cutoff510 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on October 3, 2025