Name: Helixyte™ Green dsDNA Quantifying Reagent *200X DMSO Solution*
Brand: AAT Bioquest
SKU: 17597
Availability: InStock

Helixyte™ Green dsDNA Quantifying Reagent

200X DMSO Solution

Helixyte™ Green is an excellent nucleic acid sensor that exhibits large fluorescence enhancement upon binding to dsDNA. It has the same spectral properties to those of PicoGreen®, thus a great replacement to PicoGreen® (PicoGreen® is the trademark of Invitrogen). The most commonly used technique for measuring nucleic acid concentration is the determination of absorbance at 260 nm (A260). However, the absorbance method suffers great interferences resulted from various contaminants commonly found in nucleic acid preparations, including nucleotides, single-stranded nucleic acids and proteins. Helixyte™ Green dsDNA Quantifying Reagent is an excellent alternative for quantifying DNAs with greatly improved sensitivity and selectivity. Helixyte™ Green dsDNA Quantifying Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biological procedures such as cDNA synthesis for library production and DNA fragment purification for subcloning, as well as diagnostic applications, such as quantitating DNA amplification products and primer extension assays. Using the Helixyte™ Green dsDNA Quantifying Reagent, you can selectively detect as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. Helixyte™ Green dsDNA Quantifying Reagent has a few orders of magnitude more sensitive than the UV absorbance readings, saving on precious sample. It is specific for dsDNA in the presence of equimolar amounts of RNA.

Comparison of calf thymus DNA dose response with Helixyte Green™ (blue circle) and PicoGreen® (red square) in a solid black 96-well microplate using a Gemini fluorescence microplate reader.

Protocol

Catalog	Size	Price	Quantity
17597	1 ml	Price
17598	10 ml	Price

Citations

View all 9 citations: Citation Explorer

Metagenomic insights into modulation of microbiota community composition, potential metabolic pathways and antimicrobial resistance mechanisms in cage-cultured hybrid grouper (Epinephelus fuscoguttatus♀$\times$ Epinephelus lanceolatus♂) upon oral immunizat

Authors:

Chin, Yong Kit and Ina-Salwany, Md Yasin and Mohamad, Aslah and Amir-Danial, Zahaludin and Batrisyia, Syazana-Faridon and Nazarudin, Muhammad Farhan and Nor, Norhariani Mohd and Isnansetyo, Alim and Istiqomah, Indah and Amal, Mohammad Noor Azmai and others,

Journal:

Aquaculture Reports (2026): 103574

Modulatory Effects of Isolated Lactobacillus paracasei from Malaysian Water Kefir Grains on the Intestinal Barrier and Gut Microbiota in Diabetic Mice

Authors:

Talib, Noorshafadzilah and Mohamad, Nurul Elyani and Ho, Chai Ling and Masarudin, Mas Jaffri and Alitheen, Noorjahan Banu

Journal:

Probiotics and Antimicrobial Proteins (2024): 1--13

Arthropods, nematodes, fungi, and bacteria associated with penguin carrion in Barton Peninsula, King George Island, Antarctica

Authors:

ZAINI, NUR ADILLA and Low, Van Lun and Gebrelassie, Selam Solomon and Ismail, Siti Sofo and Mahmud, Mohd Hafizi and Houssaini, Jamal and Lee, Won Young and Tomberlin, Jeffery K and Heo, Chong Chin

Bovine Fibroblast-Derived Extracellular Matrix Promotes the Growth and Preserves the Stemness of Bovine Stromal Cells during In Vitro Expansion

Authors:

Lee, Kathleen and Jackson, Anisha and John, Nikita and Zhang, Ryan and Ozhava, Derya and Bhatia, Mohit and Mao, Yong

Journal:

Journal of Functional Biomaterials (2023): 218

Faecal DNA metabarcoding reveals novel bacterial community patterns of critically endangered Southern River Terrapin, Batagur affinis

Authors:

Salleh, Mohd Hairul Mohd and Esa, Yuzine and Ngalimat, Mohamad Syazwan and Chen, Pelf Nyok

Journal:

PeerJ (2022): e12970

References

View all 31 references: Citation Explorer

Inhibitors of Streptococcus pneumoniae surface endonuclease EndA discovered by high-throughput screening using a PicoGreen fluorescence assay

Authors:

Peterson EJ, Kireev D, Moon AF, Midon M, Janzen WP, Pingoud A, Pedersen LC, Singleton SF.

Journal:

J Biomol Screen (2013): 247

Validation of a PicoGreen-based DNA quantification integrated in an RNA extraction method for two-dimensional and three-dimensional cell cultures

Authors:

Chen Y, Sonnaert M, Roberts SJ, Luyten FP, Schrooten J.

Journal:

Tissue Eng Part C Methods (2012): 444

Characterization of PicoGreen interaction with dsDNA and the origin of its fluorescence enhancement upon binding

Authors:

Dragan AI, Casas-Finet JR, Bishop ES, Strouse RJ, Schenerman MA, Geddes CD.

Journal:

Biophys J (2010): 3010

Comparison of SYBR Green I-, PicoGreen-, and [3H]-hypoxanthine-based assays for in vitro antimalarial screening of plants from Nigerian ethnomedicine

Authors:

Abiodun OO, Gbotosho GO, Ajaiyeoba EO, Happi CT, Hofer S, Wittlin S, Sowunmi A, Brun R, Oduola AM.

Journal:

Parasitol Res (2010): 933

Metal-enhanced PicoGreen fluorescence: application to fast and ultra-sensitive pg/ml DNA quantitation

Authors:

Dragan AI, Bishop ES, Casas-Finet JR, Strouse RJ, Schenerman MA, Geddes CD.

Journal:

J Immunol Methods (2010): 95

Physical properties

Molecular weight	661.17
Solvent	DMSO

Spectral properties

Excitation (nm)	502
Emission (nm)	522

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microplate reader
Excitation	490
Emission	525
Cutoff	515
Recommended plate	Solid black

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Page updated on July 16, 2026