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mFluor™ Blue 580 SE
Advances in spectral flow cytometers have expanded applications and capabilities beyond conventional flow cytometry. Now with spectral flow cytometry analysis, researchers and scientists can investigate an increasing number of molecules of interest. However, the potential of spectral flow cytometry is severely limited by the availability of fluorescent labels and readouts. AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications, in particular, for spectral fluorescence flow cytometry. mFluor™ Blue 580 dye can be well excited with blue laser at 488 nm. It has a huge Stokes shift with emission ~580 nm. mFluor™ Blue 580 dyes are water-soluble, and the protein conjugates prepared with mFluor™ Blue 580 dyes are well excited at 488 nm to give red fluorescence. mFluor™ Blue 580 dye and conjugates are excellent blue laser reagents for flow cytometry detections. Compared to RPE, mFluor™ Blue 580 dyes are much more photostable, making them readily available for fluorescence imaging applications while it is very difficult to use RPE conjugates for fluorescence imaging applications due to the rapid photobleaching of RPE conjugates. It is also a unique fluorochrome for spectral flow cytometry since there are very few existing dyes that have this spectral profile.
Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide, or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide, or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11781 mg
Price
 
Physical properties
Molecular weight832.98
SolventDMSO
Spectral properties
Absorbance (nm)492
Correction factor (260 nm)0.363
Correction factor (280 nm)0.247
Extinction coefficient (cm -1 M -1)
40000
1
Excitation (nm)485
Emission (nm)580
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 18, 2025