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mFluor™ Blue 580 Styramide
The Power Styramide™ Signal Amplification (PSA™) system is a highly sensitive method for detecting low-abundant targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH). By utilizing bright and photostable mFluor™ dyes, Styramide™ conjugates deliver results of unparalleled sharpness and precision, surpassing the sensitivity of standard ICC/IHC/ISH methods by over 100 times, all the while reducing the consumption of primary antibodies. Like tyramide signal amplification (TSA), PSA™ leverages the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. The enhanced reactivity of Styramide™ radicals over tyramide ensures faster, more robust labeling of the target, leading to fluorescence signals that are 10-50 times greater than those generated by tyramide (TSA) reagents. The mFluor™ Blue 580 Styramide uses the bright red-orange fluorescent dye mFluor™ Blue 580 (Ex/Em = 485/580 nm) to label targets in situ. mFluor™ Blue 580 is well-excited by the 488 nm laser line and exhibits minimal crosstalk in complex multicolor analysis with blue and green fluorescent probes.
Formalin-fixed, paraffin-embedded (FFPE) human lung tissue was labeled with anti-EpCAM mouse mAb followed by HRP-labeled goat anti-mouse IgG (Cat No. 16728). The fluorescence signal was developed using mFluor™ Blue 580 styramide and detected with a Cy3 filter set.
Formalin-fixed, paraffin-embedded (FFPE) human lung tissue was labeled with anti-EpCAM mouse mAb followed by HRP-labeled goat anti-mouse IgG (Cat No. 16728). The fluorescence signal was developed using mFluor™ Blue 580 styramide and detected with a Cy3 filter set.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
45071100 Slides
Price
 
Physical properties
SolventDMSO
Spectral properties
Absorbance (nm)492
Correction factor (260 nm)0.363
Correction factor (280 nm)0.247
Extinction coefficient (cm -1 M -1)
40000
1
Excitation (nm)485
Emission (nm)580
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microscope
Excitation485 nm
Emission580 nm
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 26, 2025