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mFluor™ Violet 510 goat anti-mouse IgG (H+L) *Cross-Absorbed*

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Physical properties
Molecular weight~150 kDa
Spectral properties
Absorbance (nm)410
Correction Factor (260 nm)0.464
Correction Factor (280 nm)0.366
Extinction coefficient (cm -1 M -1)250001
Excitation (nm)412
Emission (nm)505
Quantum yield0.861
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
~150 kDa
Absorbance (nm)
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
mFluor™ Violet 510 goat anti-mouse conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards mouse IgG, conjugated to the bright and stable mFluor™ Violet 510 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of mouse primary antibodies and the exceptional contrast provided by mFluor™ Violet 510 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ Violet 510 is optimally excited by the violet laser and emits bright green fluorescence maximally at 505 nm. These affinity-purified goat anti-mouse secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications. To minimize cross-reactivity, these goat anti-mouse IgG whole antibodies have been cross-adsorbed against human, horse, mouse, and bovine IgG.


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Spectral properties

Absorbance (nm)410
Correction Factor (260 nm)0.464
Correction Factor (280 nm)0.366
Extinction coefficient (cm -1 M -1)250001
Excitation (nm)412
Emission (nm)505
Quantum yield0.861

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 510 goat anti-rabbit IgG (H+L)4125052500010.8610.4640.366
mFluor™ Violet 510 goat anti-rabbit IgG (H+L) *Cross-Absorbed*4125052500010.8610.4640.366


View all 29 references: Citation Explorer
FRET causing misleading signal from fluorescein excited by the violet laser in flow cytometry.
Authors: Waeckel, Louis and Khenine, Hana and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2023)
Evaluation of a flow cytometric test for G6PD-deficient erythrocytes.
Authors: Kapadia, Alpeshkumar Bipinbhai and Sharma, Prashant and Jain, Karuna and Sachdeva, Man Updesh Singh and Bose, Parveen Lata and Gupta, Minakshi and Khadwal, Alka Rani and Bal, Amanjit and Das, Reena and Varma, Neelam
Journal: Tropical medicine & international health : TM & IH (2021): 462-468
Fluorochrome choices for multi-color flow cytometry.
Authors: Flores-Montero, Juan and Kalina, Tomas and Corral-Mateos, Alba and Sanoja-Flores, Luzalba and Pérez-Andrés, Martin and Martin-Ayuso, Marta and Sedek, Lukasz and Rejlova, Katerina and Mayado, Andrea and Fernández, Paula and van der Velden, Vincent and Bottcher, Sebastian and van Dongen, Jaques J M and Orfao, Alberto
Journal: Journal of immunological methods (2019): 112618
Improved panels for clinical immune phenotyping: Utilization of the violet laser.
Authors: Ryherd, Mark and Plassmeyer, Matthew and Alexander, Connor and Eugenio, Ines and Kleschenko, Yuliya and Badger, Ariel and Gupta, Raavi and Alpan, Oral and Sønder, Søren Ulrik
Journal: Cytometry. Part B, Clinical cytometry (2018): 671-679
Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples.
Authors: Boesch, Maximilian and Hoflehner, Elisabeth and Wolf, Dominik and Gastl, Guenther and Sopper, Sieghart
Journal: Journal of visualized experiments : JoVE (2017)
Optimized Stem Cell Detection Using the DyeCycle-Triggered Side Population Phenotype.
Authors: Boesch, Maximilian and Wolf, Dominik and Sopper, Sieghart
Journal: Stem cells international (2016): 1652389
Bioconjugatable, PEGylated Hydroporphyrins for Photochemistry and Photomedicine. Narrow-Band, Red-Emitting Chlorins.
Authors: Liu, Mengran and Chen, Chih-Yuan and Mandal, Amit Kumar and Chandrashaker, Vanampally and Evans-Storms, Rosemary B and Pitner, J Bruce and Bocian, David F and Holten, Dewey and Lindsey, Jonathan S
Journal: New journal of chemistry = Nouveau journal de chimie (2016): 7721-7740
The use of fluorescent target arrays for assessment of T cell responses in vivo.
Authors: Quah, Benjamin J C and Wijesundara, Danushka K and Ranasinghe, Charani and Parish, Christopher R
Journal: Journal of visualized experiments : JoVE (2014): e51627
Technical issues: flow cytometry and rare event analysis.
Authors: Hedley, B D and Keeney, M
Journal: International journal of laboratory hematology (2013): 344-50
Stem cell identification by DyeCycle Violet side population analysis.
Authors: Telford, William G
Journal: Methods in molecular biology (Clifton, N.J.) (2013): 163-79