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mFluor™ Violet 510 goat anti-rabbit IgG (H+L)

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Physical properties
Molecular weight~150 kDa
SolventWater
Spectral properties
Absorbance (nm)410
Correction Factor (260 nm)0.464
Correction Factor (280 nm)0.366
Extinction coefficient (cm -1 M -1)250001
Excitation (nm)412
Emission (nm)505
Quantum yield0.861
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Molecular weight
~150 kDa
Absorbance (nm)
410
Correction Factor (260 nm)
0.464
Correction Factor (280 nm)
0.366
Extinction coefficient (cm -1 M -1)
250001
Excitation (nm)
412
Emission (nm)
505
Quantum yield
0.861
mFluor™ Violet 510 goat anti-rabbit conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards rabbit IgG, conjugated to the bright and stable mFluor™ Violet 510 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of rabbit primary antibodies and the exceptional contrast provided by mFluor™ Violet 510 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ Violet 510 is optimally excited by the violet laser and emits bright green fluorescence maximally at 505 nm. These affinity-purified goat anti-rabbit secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications.

Spectrum


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spectrum

Spectral properties

Absorbance (nm)410
Correction Factor (260 nm)0.464
Correction Factor (280 nm)0.366
Extinction coefficient (cm -1 M -1)250001
Excitation (nm)412
Emission (nm)505
Quantum yield0.861

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 510 goat anti-mouse IgG (H+L)4125052500010.8610.4640.366
mFluor™ Violet 510 goat anti-mouse IgG (H+L) *Cross-Absorbed*4125052500010.8610.4640.366

References


View all 6 references: Citation Explorer
The development of fluorescent protein tracing vectors for multicolor imaging of clinically isolated Staphylococcus aureus.
Authors: Kato, Fuminori and Nakamura, Motoki and Sugai, Motoyuki
Journal: Scientific reports (2017): 2865
[Expression of Cocktail DNA Vaccine Comprising Toxoplasma gondii SAGI, MIC3 and ROP2 Using Fluorescent Protein-Reporting Vectors and Evaluation of Its Immunogenicity].
Authors: WANG, Yan-juan and CAO, Jian-ping and SUN, Ya-wen and XU, Yu-xin and SHEN, Yu-juan
Journal: Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (2015): 368-71, 376
New violet-excitable reagents for multicolor flow applications.
Authors: Abrams, Barny and Diwu, Zhenjun and Guryev, Oleg and Suni, Maria and Dubrovsky, Tim
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2013): 752-62
Use of a new violet-excitable AmCyan variant as a label in cell analysis.
Authors: Guryev, Oleg and Jaimes, Maria C and Edinger, Mark G and Matvienko, Marta and Abrams, Barny and Dubrovsky, Tim
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2012): 627-34
The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.
Authors: Cannizzo, Elisa and Carulli, Giovanni and Del Vecchio, Luigi and Ottaviano, Virginia and Bellio, Emanuele and Zenari, Ezio and Azzarà, Antonio and Petrini, Mario and Preffer, Frederic
Journal: American journal of clinical pathology (2012): 377-86
Quantum dots in flow cytometry.
Authors: Abrams, Barnaby and Dubrovsky, Tim
Journal: Methods in molecular biology (Clifton, N.J.) (2007): 185-203