Name: mFluor™ Violet 510 goat anti-rabbit IgG (H+L)
Brand: AAT Bioquest
SKU: 49250
Availability: InStock

mFluor™ Violet 510 goat anti-rabbit IgG (H+L)

mFluor™ Violet 510 goat anti-rabbit conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards rabbit IgG, conjugated to the bright and stable mFluor™ Violet 510 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of rabbit primary antibodies and the exceptional contrast provided by mFluor™ Violet 510 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ Violet 510 is optimally excited by the violet laser and emits bright green fluorescence maximally at 505 nm. These affinity-purified goat anti-rabbit secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications.

Datasheet

SDS

Catalog	Size	Price	Quantity
49250	200 ug	Price
49251	1 mg	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of IgG antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

References

View all 6 references: Citation Explorer

The development of fluorescent protein tracing vectors for multicolor imaging of clinically isolated Staphylococcus aureus.

Authors:

Kato, Fuminori and Nakamura, Motoki and Sugai, Motoyuki

Journal:

Scientific reports (2017): 2865

[Expression of Cocktail DNA Vaccine Comprising Toxoplasma gondii SAGI, MIC3 and ROP2 Using Fluorescent Protein-Reporting Vectors and Evaluation of Its Immunogenicity].

Authors:

WANG, Yan-juan and CAO, Jian-ping and SUN, Ya-wen and XU, Yu-xin and SHEN, Yu-juan

Journal:

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (2015): 368-71, 376

New violet-excitable reagents for multicolor flow applications.

Authors:

Abrams, Barny and Diwu, Zhenjun and Guryev, Oleg and Suni, Maria and Dubrovsky, Tim

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2013): 752-62

Use of a new violet-excitable AmCyan variant as a label in cell analysis.

Authors:

Guryev, Oleg and Jaimes, Maria C and Edinger, Mark G and Matvienko, Marta and Abrams, Barny and Dubrovsky, Tim

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2012): 627-34

The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.

Authors:

Cannizzo, Elisa and Carulli, Giovanni and Del Vecchio, Luigi and Ottaviano, Virginia and Bellio, Emanuele and Zenari, Ezio and Azzarà, Antonio and Petrini, Mario and Preffer, Frederic

Journal:

American journal of clinical pathology (2012): 377-86

Physical properties

Molecular weight	~150 kDa
Solvent	Water

Antibody properties

Host	Goat
Reactivity	Rabbit

Spectral properties

Absorbance (nm)	410
Correction factor (260 nm)	0.464
Correction factor (280 nm)	0.366
Extinction coefficient (cm ^-1 M ^-1)	25000 ¹
Excitation (nm)	412
Emission (nm)	505
Quantum yield	0.86 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
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Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on May 4, 2026