mFluor™ Violet 510 goat anti-rabbit IgG (H+L) *Cross-Absorbed*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~150 kDa |
Solvent | Water |
Spectral properties
Absorbance (nm) | 410 |
Correction Factor (260 nm) | 0.464 |
Correction Factor (280 nm) | 0.366 |
Extinction coefficient (cm -1 M -1) | 250001 |
Excitation (nm) | 412 |
Emission (nm) | 505 |
Quantum yield | 0.861 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
mFluor™ Violet 510 goat anti-rabbit IgG (H+L) |
Overview | SDSProtocol |
Molecular weight ~150 kDa | Absorbance (nm) 410 | Correction Factor (260 nm) 0.464 | Correction Factor (280 nm) 0.366 | Extinction coefficient (cm -1 M -1) 250001 | Excitation (nm) 412 | Emission (nm) 505 | Quantum yield 0.861 |
mFluor™ Violet 510 goat anti-rabbit conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards rabbit IgG, conjugated to the bright and stable mFluor™ Violet 510 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of rabbit primary antibodies and the exceptional contrast provided by mFluor™ Violet 510 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ Violet 510 is optimally excited by the violet laser and emits bright green fluorescence maximally at 505 nm. These affinity-purified goat anti-rabbit secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications. To minimize cross-reactivity, these goat anti-rabbit IgG whole antibodies have been cross-adsorbed against human, horse, rabbit, and bovine IgG.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 410 |
Correction Factor (260 nm) | 0.464 |
Correction Factor (280 nm) | 0.366 |
Extinction coefficient (cm -1 M -1) | 250001 |
Excitation (nm) | 412 |
Emission (nm) | 505 |
Quantum yield | 0.861 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
mFluor™ Violet 510 goat anti-mouse IgG (H+L) | 412 | 505 | 250001 | 0.861 | 0.464 | 0.366 |
mFluor™ Violet 510 goat anti-mouse IgG (H+L) *Cross-Absorbed* | 412 | 505 | 250001 | 0.861 | 0.464 | 0.366 |
References
View all 4 references: Citation Explorer
FRET causing misleading signal from fluorescein excited by the violet laser in flow cytometry.
Authors: Waeckel, Louis and Khenine, Hana and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2023)
Authors: Waeckel, Louis and Khenine, Hana and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2023)
The use of fluorescent target arrays for assessment of T cell responses in vivo.
Authors: Quah, Benjamin J C and Wijesundara, Danushka K and Ranasinghe, Charani and Parish, Christopher R
Journal: Journal of visualized experiments : JoVE (2014): e51627
Authors: Quah, Benjamin J C and Wijesundara, Danushka K and Ranasinghe, Charani and Parish, Christopher R
Journal: Journal of visualized experiments : JoVE (2014): e51627
Use of a new violet-excitable AmCyan variant as a label in cell analysis.
Authors: Guryev, Oleg and Jaimes, Maria C and Edinger, Mark G and Matvienko, Marta and Abrams, Barny and Dubrovsky, Tim
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2012): 627-34
Authors: Guryev, Oleg and Jaimes, Maria C and Edinger, Mark G and Matvienko, Marta and Abrams, Barny and Dubrovsky, Tim
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2012): 627-34
Quantum dots in flow cytometry.
Authors: Abrams, Barnaby and Dubrovsky, Tim
Journal: Methods in molecular biology (Clifton, N.J.) (2007): 185-203
Authors: Abrams, Barnaby and Dubrovsky, Tim
Journal: Methods in molecular biology (Clifton, N.J.) (2007): 185-203