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mFluor™ Violet 540 Biotin Conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1504.80 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 401 |
| Correction Factor (260 nm) | 1.326 |
| Correction Factor (280 nm) | 0.543 |
| Extinction coefficient (cm -1 M -1) | 180001 |
| Excitation (nm) | 402 |
| Emission (nm) | 535 |
| Quantum yield | 0.211 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
See also: mFluor™ Dyes and Kits
Molecular weight 1504.80 | Absorbance (nm) 401 | Correction Factor (260 nm) 1.326 | Correction Factor (280 nm) 0.543 | Extinction coefficient (cm -1 M -1) 180001 | Excitation (nm) 402 | Emission (nm) 535 | Quantum yield 0.211 |
mFluor™ Violet 540 Biotin Conjugate contains mFluor™ Violet 540 fluorophore that is well excited by the common 405 nm violet laser. It has a similar spectrum to Pacific Orange and demonstrates excellent avidin binding and strong orange fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Violet 540 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 540 Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 66.454 µL | 332.27 µL | 664.54 µL | 3.323 mL | 6.645 mL |
| 5 mM | 13.291 µL | 66.454 µL | 132.908 µL | 664.54 µL | 1.329 mL |
| 10 mM | 6.645 µL | 33.227 µL | 66.454 µL | 332.27 µL | 664.54 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 401 |
| Correction Factor (260 nm) | 1.326 |
| Correction Factor (280 nm) | 0.543 |
| Extinction coefficient (cm -1 M -1) | 180001 |
| Excitation (nm) | 402 |
| Emission (nm) | 535 |
| Quantum yield | 0.211 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| mFluor™ Violet 540-streptavidin conjugate | 402 | 535 | 180001 | 0.211 | 1.326 | 0.543 |
| mFluor™ Violet 500 Biotin Conjugate | 410 | 501 | 250001 | 0.811 | 0.769 | 0.365 |
Images
Figure 1. mFluor™ Violet 540 Biotin Conjugate contains mFluor™ Violet 540 fluorophore that is well excited by the common 405 nm violet laser. It has a similar spectrum to Pacific Orange and demonstrates excellent avidin binding and strong orange fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers.
References
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