mFluor™ Violet 540 Biotin Conjugate

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1504.80
Solvent	DMSO

Spectral properties

Absorbance (nm)	401
Correction Factor (260 nm)	1.326
Correction Factor (280 nm)	0.543
Extinction coefficient (cm ^-1 M ^-1)	18000¹
Excitation (nm)	402
Emission (nm)	535
Quantum yield	0.21¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

1504.80

Absorbance (nm)

401

Correction Factor (260 nm)

1.326

Correction Factor (280 nm)

0.543

Extinction coefficient (cm ^-1 M ^-1)

18000¹

Excitation (nm)

402

Emission (nm)

535

Quantum yield

0.21¹

mFluor™ Violet 540 Biotin Conjugate contains mFluor™ Violet 540 fluorophore that is well excited by the common 405 nm violet laser. It has a similar spectrum to Pacific Orange and demonstrates excellent avidin binding and strong orange fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Violet 540 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 540 Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	66.454 µL	332.27 µL	664.54 µL	3.323 mL	6.645 mL
5 mM	13.291 µL	66.454 µL	132.908 µL	664.54 µL	1.329 mL
10 mM	6.645 µL	33.227 µL	66.454 µL	332.27 µL	664.54 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	401
Correction Factor (260 nm)	1.326
Correction Factor (280 nm)	0.543
Extinction coefficient (cm ^-1 M ^-1)	18000¹
Excitation (nm)	402
Emission (nm)	535
Quantum yield	0.21¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
mFluor™ Violet 540-streptavidin conjugate	402	535	18000¹	0.21¹	1.326	0.543
mFluor™ Violet 500 Biotin Conjugate	410	501	25000¹	0.81¹	0.769	0.365

Images

Figure 1. mFluor™ Violet 540 Biotin Conjugate contains mFluor™ Violet 540 fluorophore that is well excited by the common 405 nm violet laser. It has a similar spectrum to Pacific Orange and demonstrates excellent avidin binding and strong orange fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers.

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