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mFluor™ Violet 540 Biotin Conjugate

mFluor™ Violet 540 Biotin Conjugate contains mFluor™ Violet 540 fluorophore that is well excited by the common 405 nm violet laser. It has a similar spectrum to Pacific Orange and demonstrates excellent avidin binding and strong orange fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers.
mFluor™ Violet 540 Biotin Conjugate contains mFluor™ Violet 540 fluorophore that is well excited by the common 405 nm violet laser. It has a similar spectrum to Pacific Orange and demonstrates excellent avidin binding and strong orange fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers.
Ordering information
Price ()
Catalog Number3114
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1504.80
SolventDMSO
Spectral properties
Absorbance (nm)401
Correction Factor (260 nm)1.326
Correction Factor (280 nm)0.543
Extinction coefficient (cm -1 M -1)180001
Excitation (nm)402
Emission (nm)535
Quantum yield0.211
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Show More (108)

OverviewpdfSDSpdfProtocol


Molecular weight
1504.80
Absorbance (nm)
401
Correction Factor (260 nm)
1.326
Correction Factor (280 nm)
0.543
Extinction coefficient (cm -1 M -1)
180001
Excitation (nm)
402
Emission (nm)
535
Quantum yield
0.211
mFluor™ Violet 540 Biotin Conjugate contains mFluor™ Violet 540 fluorophore that is well excited by the common 405 nm violet laser. It has a similar spectrum to Pacific Orange and demonstrates excellent avidin binding and strong orange fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Violet 540 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Violet 540 Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM66.454 µL332.27 µL664.54 µL3.323 mL6.645 mL
5 mM13.291 µL66.454 µL132.908 µL664.54 µL1.329 mL
10 mM6.645 µL33.227 µL66.454 µL332.27 µL664.54 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)401
Correction Factor (260 nm)1.326
Correction Factor (280 nm)0.543
Extinction coefficient (cm -1 M -1)180001
Excitation (nm)402
Emission (nm)535
Quantum yield0.211

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 540-streptavidin conjugate4025351800010.2111.3260.543
mFluor™ Violet 500 Biotin Conjugate4105012500010.8110.7690.365

References


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Journal: Journal of drug targeting (2022): 1-9
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Authors: Towner, Rheal A and Saunders, Debra and Lerner, Megan and Silasi Mansat, Robert and Yuan, Tian and Barber, Dylan and Faakye, Janet and Nyul-Toth, Adam and Csiszar, Anna and Greenwood-Van Meerveld, Beverley and Smith, Nataliya
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Authors: Reisbeck, Felix and Wedepohl, Stefanie and Dimde, Mathias and Schmitt, Ann-Cathrin and Dernedde, Jens and Álvaro-Benito, Miguel and Freund, Christian and Haag, Rainer
Journal: Journal of materials chemistry. B (2021): 96-106
In Vitro and In Situ Activity-Based Labeling of Fibroblast Activation Protein with UAMC1110-Derived Probes.
Authors: Van Rymenant, Yentl and Tanc, Muhammet and Van Elzen, Roos and Bracke, An and De Wever, Olivier and Augustyns, Koen and Lambeir, Anne-Marie and Kockx, Mark and De Meester, Ingrid and Van Der Veken, Pieter
Journal: Frontiers in chemistry (2021): 640566
Crystal Structure-Guided Design of Bisubstrate Inhibitors and Photoluminescent Probes for Protein Kinases of the PIM Family.
Authors: Nonga, Olivier E and Lavogina, Darja and Enkvist, Erki and Kestav, Katrin and Chaikuad, Apirat and Dixon-Clarke, Sarah E and Bullock, Alex N and Kopanchuk, Sergei and Ivan, Taavi and Ekambaram, Ramesh and Viht, Kaido and Knapp, Stefan and Uri, Asko
Journal: Molecules (Basel, Switzerland) (2021)
High-throughput immunosensor chip coupled with a fluorescent DNA dendrimer for ultrasensitive detection of cardiac troponin T.
Authors: Wang, Ruike and Zong, Chen and Li, Gairu and Wang, Junhong and Kong, Tiantian and Li, Fei and Chang, Junmin
Journal: RSC advances (2021): 27523-27529
Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at the single-molecule level.
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