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mFluor™ Violet 540 Biotin Conjugate
mFluor™ Violet 540 Biotin Conjugate contains mFluor™ Violet 540 fluorophore that is well excited by the common 405 nm violet laser. It has a similar spectrum to Pacific Orange and demonstrates excellent avidin binding and strong orange fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Violet 540 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| mFluor™ Violet 500 Biotin Conjugate | 410 | 501 | 250001 | 0.811 | 0.769 | 0.365 |
| mFluor™ Violet 540-streptavidin conjugate | 402 | 535 | 180001 | 0.211 | 1.326 | 0.543 |
References
View all 50 references: Citation Explorer
Black phosphorus nanoparticles for dual therapy of non-small cell lung cancer.
Authors: Lin, Zhongxiao and Deng, Qiudi and Fang, Qi and Li, Xinzhi and Liu, Xiaoyan and Wang, Jianglin and Chen, Sheng and Huang, Xiaotao and Yang, Langyu and Miao, Yingling and Yu, Xi-Yong
Journal: Journal of drug targeting (2022): 1-9
Authors: Lin, Zhongxiao and Deng, Qiudi and Fang, Qi and Li, Xinzhi and Liu, Xiaoyan and Wang, Jianglin and Chen, Sheng and Huang, Xiaotao and Yang, Langyu and Miao, Yingling and Yu, Xi-Yong
Journal: Journal of drug targeting (2022): 1-9
Investigation and validation of labelling loop mediated isothermal amplification (LAMP) products with different nucleotide modifications for various downstream analysis.
Authors: Warmt, Christian and Yaslanmaz, Ceren and Henkel, Jörg
Journal: Scientific reports (2022): 7137
Authors: Warmt, Christian and Yaslanmaz, Ceren and Henkel, Jörg
Journal: Scientific reports (2022): 7137
A ratiometric fluorescent nanoprobe for signal amplification monitoring of intracellular telomerase activity.
Authors: Ma, Dandan and Bai, Huiyun and Li, Junbo and Li, Yintao and Song, Lihua and Zheng, Jinping and Miao, Congxiu
Journal: Analytical and bioanalytical chemistry (2022): 1891-1898
Authors: Ma, Dandan and Bai, Huiyun and Li, Junbo and Li, Yintao and Song, Lihua and Zheng, Jinping and Miao, Congxiu
Journal: Analytical and bioanalytical chemistry (2022): 1891-1898
A low-noise ratiometric fluorescence biosensor for detection of Pb2+ based on DNAzyme and exonuclease III-assisted cascade signal amplification.
Authors: Jin, Huali and Liu, Ruike and Bai, Tian and Wei, Min and He, Baoshan and Suo, Zhiguang
Journal: Analytical and bioanalytical chemistry (2022): 1899-1907
Authors: Jin, Huali and Liu, Ruike and Bai, Tian and Wei, Min and He, Baoshan and Suo, Zhiguang
Journal: Analytical and bioanalytical chemistry (2022): 1899-1907
Detection of cholera toxin with surface plasmon field-enhanced fluorescent spectroscopy.
Authors: Seherler, Sebnem and Bozdogan, Anil and Ozal Ildeniz, Tugba Arzu and Kok, Fatma Nese and Anac Sakir, Ilke
Journal: Biotechnology and applied biochemistry (2021)
Authors: Seherler, Sebnem and Bozdogan, Anil and Ozal Ildeniz, Tugba Arzu and Kok, Fatma Nese and Anac Sakir, Ilke
Journal: Biotechnology and applied biochemistry (2021)
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