MycoLight™ Fluorescence Live/Dead Bacterial Imaging Kit

AAT Bioquest's MycoLight™ Fluorescence Live/Dead Bacterial Imaging Kit provides two-color fluorescence assay for visualizing live and dead bacteria through fluorescent microscope. MycoLight™ 520 is a non-fluorescent esterase substrate that diffuses into both Gram positive and Gram-negative bacteria. Upon hydrolysis by bacterial intracellular non-specific esterases, a green fluorescent product is produced and accumulated within bacteria. In contrast, propidium iodide is a red-fluorescent nucleic acid stain that only penetrates bacteria with damaged membranes. Thus, with an appropriate mixture of the MycoLight™ 520 and propidium iodide stains, live bacteria with intact cell membranes emits green fluorescence, whereas dead bacteria with damaged membranes gives red fluorescence. The MycoLight™ Fluorescence Live/Dead Bacterial Imaging Kit is a robust tool for imaging Live/Dead bacteria. Stained cells can be monitored fluorimeterically (FITC filter set) and (TRITC filter set) for live and dead bacteria respectively.

Protocol

SDS

Catalog	Size	Price	Quantity
22411	100 Tests	Price

Citations

View all 4 citations: Citation Explorer

Antibacterial mechanism of Lactiplantibacillus plantarum SHY96 cell-free supernatant against Listeria monocytogenes revealed by metabolomics and potential application on chicken breast meat preservation

Authors:

Liang, Ming and Wang, Hongwei and Zhou, Zhaoquan and Huang, Yechuan and Suo, Huayi

Journal:

Food Chemistry: X (2024): 102078

Response of Human Gingival Fibroblasts and Porphyromonas gingivalis to UVC-Activated Titanium Surfaces

Authors:

Wen, Yin and Dong, Hao and Lin, Jiating and Zhuang, Xianxian and Xian, Ruoting and Li, Ping and Li, Shaobing

Journal:

Journal of Functional Biomaterials (2023): 137

Anti-Inflammatory and Repairing Effects of Mesoporous Silica-Loaded Metronidazole Composite Hydrogel on Human Dental Pulp Cells

Authors:

Wang, Wei and Wang, Xixi and Li, Luyang and Liu, Ying

Journal:

Journal of Healthcare Engineering (2022)

Fabrication of PAMAM antimicrobial monolayer via UV induced grafting on the surface of polyethylene terephthalate

Authors:

Zhang, Haobo and Wang, Weihan and Wei, Lilong and Wu, Dezhen and Cheng, Jue and Gao, Feng

Journal:

Colloids and Surfaces B: Biointerfaces (2021): 111601

References

View all 16 references: Citation Explorer

Raman spectroscopic analysis of Lactobacillus rhamnosus GG in response to dehydration reveals DNA conformation changes

Authors:

Myintzu Hlaing, M.; Wood, B.; McNaughton, D.; Ying, D.; Augustin, M. A.

Journal:

J Biophotonics (2017): 589-597

Inactivation of Cronobacter sakazakii in reconstituted infant formula by combination of thymoquinone and mild heat

Authors:

Shi, C.; Jia, Z.; Chen, Y.; Yang, M.; Liu, X.; Sun, Y.; Zheng, Z.; Zhang, X.; Song, K.; Cui, L.; Baloch, A. B.; Xia, X.

Journal:

J Appl Microbiol (2015): 1700-6

Antibacterial and antigelatinolytic effects of Satureja hortensis L. essential oil on epithelial cells exposed to Fusobacterium nucleatum

Authors:

Zeidan-Chulia, F.; Keskin, M.; Kononen, E.; Uitto, V. J.; Soderling, E.; Moreira, J. C.; Gursoy, U. K.

Journal:

J Med Food (2015): 503-6

Fourier transform infra-red spectroscopy and flow cytometric assessment of the antibacterial mechanism of action of aqueous extract of garlic (Allium sativum) against selected probiotic Bifidobacterium strains

Authors:

Booyens, J.; Thantsha, M. S.

Journal:

BMC Complement Altern Med (2014): 289

Observation of injured E. coli population resulting from the application of high-pressure throttling treatments

Authors:

De Lamo-Castellvi, S.; Toledo, R.; Frank, J. F.

Journal:

J Food Sci (2013): M582-6

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	488 nm / 540 nm
Emission	530 nm / 620 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC / TRITC filter(s)

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Flyer: Live or Dead™ Cell Staining Kits

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 31, 2025

Component A: MycoLight™ 520	1 vial
Component B: Propidium iodide (100X)	1 vial (100 µL)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: DMSO	1 vial (100 µL)