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Nuclear Violet™ DCS1 *5 mM DMSO Solution*

Fluorescence image of HeLa cells fixed with 4% formaldehyde and then stained with Nuclear Violet™ DCS1.
Fluorescence image of HeLa cells fixed with 4% formaldehyde and then stained with Nuclear Violet™ DCS1.
Fluorescence image of HeLa cells fixed with 4% formaldehyde and then stained with Nuclear Violet™ DCS1.
Fixed and Live (non-fixed) HeLa cells were plated on 96-well plates, incubated with Nuclear Violet™ DCS1 1 µM for 20 minutes, and imaged with a DAPI channel.
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Physical properties
Molecular weight744.77
Spectral properties
Excitation (nm)371
Emission (nm)454
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Our Nuclear Violet™ DCS1 is a fluorogenic, DNA-selective and live cell-impermeant dye for analyzing DNA content in dead or fixed cells. The Nuclear Violet™ DCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. It is well excited by violet laser at 405 nm, and emits blue/cyan fluorescence light around an emission maximum at ~440 nm, and provides an excellent tool for flow cytometers equipped with a 405 nm violet laser source. This DNA-binding dye might be used for multicolor analysis of live/dead cells with the filter sets of Pacific Blue and BD Horizon V450.


Fluorescence microscope

ExcitationDAPI filter set
EmissionDAPI filter set
Recommended plateBlack wall/clear bottom

Example protocol


Nuclear Violet™ DCS1 working solution
Dilute the Nuclear Violet™ DCS1 stock solution (5 mM) to 0.5 to 5 µM final concentration in the buffer of your choice.


The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types and factors may influence staining. Residual detergent on glassware may also affect staining of many organisms, and cause brightly stained material to appear in solutions with or without cells present.
  1. Add Nuclear Violet™ DCS1 working solution into the fixed, dead or apoptotic cells (either suspension or adherent) and stain the cells for 15 to 60 minutes. In initial experiments, it may be best to try several dye concentrations to determine the optimal concentration that yields the desired result.
  2. Directly analyze the cellular staining with fluorescence microscopy, fluorescence microplate reader, or flow cytometry.
    Note     Optional: Cells can be washed with PBS prior to analysis. 


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nuclear Violet™ DCS1 *5 mM DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM134.27 µL671.348 µL1.343 mL6.713 mL13.427 mL
5 mM26.854 µL134.27 µL268.539 µL1.343 mL2.685 mL
10 mM13.427 µL67.135 µL134.27 µL671.348 µL1.343 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


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Spectral properties

Excitation (nm)371
Emission (nm)454



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