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Portelite™ Fluorimetric RNA Quantification Kit *5-100 ng High Sensitivity*
Product key features
Portelite™ Fluorimetric RNA Quantification Assay Kit provides accurate RNA measurement at low concentrations for sensitive applications.
- Ultra-sensitive detection: Accurately quantifies RNA in the 5–100 ng range with high signal-to-background ratio
- High specificity: Selectively measures RNA with minimal interference from DNA, proteins, or free nucleotides
- Applications: Suitable for RT-qPCR, RNA-seq, cDNA synthesis, and other RNA-based studies requiring low input amounts
- Comparable alternative: Outperforms traditional absorbance-based RNA quantification kits by enabling accurate detection at low concentrations with reduced interference
Product description
The Portelite™ Fluorimetric RNA Quantification Assay Kit offers a highly sensitive method for measuring RNA in the range of 5 to 100 ng. Using a proprietary RNA-selective fluorescent dye, this assay delivers superior accuracy and specificity over traditional absorbance-based methods by reducing interference from DNA, proteins, and free nucleotides.
Designed for molecular biology, transcriptomics, and RNA-based workflows, this kit requires minimal RNA input while providing reproducible results across multiple sample types. Its straightforward workflow improves consistency and reduces handling time. The Portelite™ Fluorimetric RNA Quantification Assay Kit is well-suited for applications such as RT-qPCR, RNA-seq, and cDNA synthesis where precision at low RNA concentrations is critical.
Example protocol
AT A GLANCE
- Add StrandBrite™ Red DNA HS reagent working solution (200 µL).
- Add test samples (10 µL).
- Incubate at room temperature for 5 minutes.
- Monitor fluorescence intensity in Qubit™ fluorometer using Red filter.
Important: The following protocol is provided as an example for quantifying total RNA with StrandBrite™ Red RNA HS. Warm all the components to room temperature before opening. No data are available for the mutagenicity or toxicity of StrandBrite™ Red RNA HS stain. Because this reagent binds to nucleic acids, it should be treated as a potential mutagen and handled with appropriate care. The DMSO stock solution should be handled with particular caution as DMSO is known to facilitate the entry of organic molecules into tissues.
PREPARATION OF WORKING SOLUTION
Add 2.5 μL StrandBrite™ Red RNA HS (Component A) into 1 mL of RNA Assay Buffer (Component B) and mix well. Protect the working solution from light by covering it with foil or placing it in the dark.
Note: 1 mL of working solution is enough for 5 tests.
Note: We recommend preparing this solution in a plastic container rather than glass, as the dye may adsorb to glass surfaces. For best results, this solution should be used within a few hours of its preparation.
SAMPLE EXPERIMENTAL PROTOCOL
The acceptable sample volume could range from 1~20 µL depending on the estimated concentration of the RNA sample. The recommended sample volume is 10 µL with the RNA concentration in 0.5~10 ng/µL range. If other sample volume is being used, please adjust the dilution factor in the concentration calculations.
The following protocol is generated based on 10 µL sample volume with the RNA concentration in 0.5-10 ng/µL range.
- Add 200 µL StrandBrite™ Red RNA HS working solution into each CytoCite™ sample tube (AAT cat# CCT100) or equivalent 0.2 mL PCR tube.
Note: Use thin-wall, polypropylene, clear 0.2 mL PCR tubes such as AAT cat# CCT100.
- Add 10 µL RNA standard #1 and #2 or test samples into each tube, and then mix by vortexing 2-3 seconds.
- Incubate all tubes at room temperature for 2 minutes.
- Insert the samples into Qubit™ and monitor the fluorescence with red fluorescence channel.
For StrandBrite™ assays, you have the choice to make a calibration curve with the RNA standards. Here is a brief protocol to generate a customized RNA standard curve:
- Perform dilution of RNA standard (Component D) to 10, 8, 6, 4, 2, 1, 0.5 ng/uL in RNA Assay Buffer (Component B).
- Add 200 µL StrandBrite™ Red DNA BR working solution into each tube.
- Add 10 µL RNA standards or test samples into each tube, and then mix by vortexing 2~3 seconds.
- Incubate the reaction at room temperature for 2 minutes.
- Insert the samples into Qubit™ and monitor the fluorescence with red fluorescence channel.
References
Authors: Tanaka, Ami and Matsubayashi, Keiji and Odajima, Takeshi and Sakata, Hidekatsu and Iida, Juri and Kai, Kazuhiro and Goto, Naoko and Satake, Masahiro
Journal: Transfusion (2024): 335-347
Authors: Dyer, Wayne B and Suzuki, Kazuo and Levert, Angelique and Starr, Mitchell and Lloyd, Andrew R and Zaunders, John J
Journal: Frontiers in immunology (2024): 1382711
Authors: Hosokawa, Kazuo and Ohmori, Hitoshi
Journal: Biochemical and biophysical research communications (2024): 151070
Authors: Qureshi, Huma and Duran, Ana Carrasco and Mahmood, Hassan and Sarwar, Zahida and Mahmood, Khalid and Midde, Krishna and Canchola, Jesse A and Parkin, Neil T and La Brot, Benjamin
Journal: Journal of viral hepatitis (2024): 156-160
Authors: Kang, Brian and Zhang, Jiayu and Schwoerer, Michael P and Nelson, Amy N and Schoeman, Emily and Guo, Andrew and Ploss, Alexander and Myhrvold, Cameron
Journal: bioRxiv : the preprint server for biology (2023)
Safety data sheet