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Portelite™ Total DNA Quantification Assay Kit *4-2000 ng Broad Range*
Product key features
Portelite™ Total DNA Quantification Assay Kit delivers accurate and contamination-resistant DNA measurement for research and diagnostic applications.
- High selectivity: Precisely quantifies dsDNA with minimal interference from RNA or proteins
- Wide detection range: Measures DNA from 0.2 to 2000 ng/µL (4–2000 ng detection range) with high accuracy
- Applications: Suitable for NGS library preparation, genomic DNA analysis, and diagnostic assay workflows
- Comparable alternative: Performs as a robust, broad-range option comparable to Qubit™ DNA BR Assay Kits
Product description
The Portelite™ Total DNA Quantification Assay Kit is optimized for precise and reliable quantitation of total DNA, offering exceptional selectivity for double-stranded DNA (dsDNA) over RNA and protein contaminants. It delivers superior stability, a broad linear dynamic range, and enhanced sensitivity compared to traditional absorbance-based DNA measurement methods.
The kit includes a concentrated quantitation reagent, optimized dilution buffer, and pre-calibrated dsDNA standards to ensure accuracy and reproducibility. It demonstrates high tolerance to proteins, salts, solvents, and detergents, enabling consistent performance across diverse sample types. The Portelite™ kit is ideal for applications such as NGS library preparation, genomic DNA quantification, and diagnostic workflows where precision and reliability are essential.
Example protocol
AT A GLANCE
Prepare Helixyte™ Green DNA BR working solution.
Add 190 µL of 1X Helixyte™ Green DNA BR working solution to each 0.2 mL PCR tube.
Add 10 µL of DNA standards or test samples to each tube.
Incubate at room temperature for 2 minutes.
Monitor fluorescence with CytoCite™ Fluorometer or Qubit™.
Note: Bring all the kit components at room temperature before starting the experiment.
PREPARATION OF WORKING SOLUTION
Make a 100-fold dilution of Helixyte™ Green DNA BR (Component A) in DNA assay buffer (Component B). For example, to prepare enough working solution for 100 samples, add 200 μL of Helixyte™ Green BR (Component A) into 20 mL of DNA Assay Buffer (Component B).
Note: Protect the working solution from light by covering it with foil or placing it in the dark.
Note: We recommend preparing this solution in a plastic container rather than glass, as the dye may adsorb to glass surfaces.
Note: Use this solution within a few hours of preparation to ensure optimal results.
SAMPLE EXPERIMENTAL PROTOCOL
Sample Volume: The acceptable sample volume can range from 1~20 μL depending on the estimate concentration of DNA sample. The recommended sample volume is 10 μL with DNA concentration of 4–2000 ng. If a different sample volumes is used, adjust the dilution factor accordingly when calculating the concentration.
The following protocol is based on 10 μL sample volume with the DNA concentration in 0.2~100 ng /µL range:
Add 190 µL of 1X Helixyte™ Green DNA BR working solution to each Cytocite™ sample tube (#CCT100) or equivalent 0.2 mL PCR tube.
Note: Use thin-wall, polypropylene, clear 0.2 mL PCR tubes such as #CCT100.
Add 10 µL of DNA standards or test samples to each tube, then mix by vortexing for 2~3 seconds.
Incubate all tubes at room temperature for 2 minutes.
Insert the samples into CytoCite™ or Qubit™ and monitor the fluorescence using the green fluorescence channel. Follow the appropriate procedure for CytoCite™ Fluorometer. See the link below for detailed instructions:
https://devices.aatbio.com/documentation/user-manual-for-cytocite-fluorometer
For Portelite™ assays, you have the choice to make a calibration curve with the DNA standards with the lower concentrations. Here is a brief protocol to generate a customized DNA standard curve:
Perform 1/3 serial dilution with 100 ng/ μL with DNA Standard BR #2 (Component D) in DNA Assay Buffer (Component B) to obtain 30, 10, 3, 1, 0.3, 0.1 and 0 ng/μL DNA standard dilutions.
Add 190 µL of Helixyte™ Green DNA BR working solution to each tube.
Add 10 µL standards or 10 µL samples to a 0.2 mL PCR tube.
Incubate the reaction at room temperature for 2 minutes.
Insert the samples into CytoCite™ and monitor the fluorescence using the green fluorescence channel.
Product family
| Name | Excitation (nm) | Emission (nm) |
| Helixyte™ Total DNA Quantification Assay Kit *4-2000 ng Broad Range* | 503 | 527 |
References
Authors: Versmessen, Nick and Van Simaey, Leen and Negash, Abel Abera and Vandekerckhove, Marjolein and Hulpiau, Paco and Vaneechoutte, Mario and Cools, Piet
Journal: PloS one (2024): e0305650
Authors: Flory, Andi and Ruiz-Perez, Carlos A and Clavere-Graciette, Ana G and Rafalko, Jill M and O'Kell, Allison L and Flesner, Brian K and McLennan, Lisa M and Hicks, Susan C and Nakashe, Prachi and Phelps-Dunn, Ashley and DiMarzio, Lauren R and Warren, Chelsea D and Cohen, Todd A and Chibuk, Jason and Chorny, Ilya and Grosu, Daniel S and Tsui, Dana W Y and Tynan, John A and Kruglyak, Kristina M
Journal: Journal of the American Veterinary Medical Association (2024): 665-673
Authors: Khuda, Fazle and Jayusman, Putri Ayu and Baharin, Badiah and Mohamad Anuar, Nur Najmi and Sharma, Anubhava and Shaqinah Nasruddin, Nurrul
Journal: Iranian journal of microbiology (2024): 337-341
Authors: Zhang, Xinmeng and Zhou, Cheng and Hou, Jianxun and Feng, Gang and Xu, Zhourui and Shao, Yonghong and Yang, Chengbin and Xu, Gaixia
Journal: Biosensors (2024)
Authors: Sinha, Rohita and Zhu, Zixuan and Park, Sookhyeon and Rebello, Christabel and Kinsella, Bradley and Friedewald, John and Kleiboeker, Steven
Journal: Transplantation proceedings (2024): 1522-1530
Safety data sheet