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Protonex™ Red 780 AM *Cell-Permeable*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 798.89 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 748 |
| Emission (nm) | 769 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
See also: Intracellular pH
Molecular weight 798.89 | Excitation (nm) 748 | Emission (nm) 769 |
Protonex™ Red 780 AM is the cell-permeable version of Protonex™ Red 780. Protonex™ Red 780 dye works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 780 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 780 is bound to an acidic intracellular target, it becomes highly fluorescent and emits near-infrared light when excited by a red laser. By measuring the fluorescence intensity of Protonex™ Red 780, one can label or monitor the acidic intracellular targets in live cells.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Red 780 AM *Cell-Permeable* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 125.174 µL | 625.868 µL | 1.252 mL | 6.259 mL | 12.517 mL |
| 5 mM | 25.035 µL | 125.174 µL | 250.347 µL | 1.252 mL | 2.503 mL |
| 10 mM | 12.517 µL | 62.587 µL | 125.174 µL | 625.868 µL | 1.252 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product Family
| Name | Excitation (nm) | Emission (nm) |
| Protonex™ Red 670 AM *Cell-Permeable* | 643 | 660 |
| Protonex™ Red 780 acid | 748 | 769 |
References
View all 50 references: Citation Explorer
mOrange2, a Genetically Encoded, pH Sensitive Fluorescent Protein, is an Alternative to BCECF-AM to Measure Intracellular pH to Determine NHE3 and DRA Activity.
Authors: Sarker, Rafiquel and Tse, Chung Ming and Lin, Ruxian and McNamara, George and Singh, Varsha and Donowitz, Mark
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2022): 39-49
Authors: Sarker, Rafiquel and Tse, Chung Ming and Lin, Ruxian and McNamara, George and Singh, Varsha and Donowitz, Mark
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2022): 39-49
Effects of Diluent pH on Enrichment and Performance of Dairy Goat X/Y Sperm.
Authors: He, Qifu and Wu, Shenghui and Huang, Ming and Wang, Ying and Zhang, Kang and Kang, Jian and Zhang, Yong and Quan, Fusheng
Journal: Frontiers in cell and developmental biology (2021): 747722
Authors: He, Qifu and Wu, Shenghui and Huang, Ming and Wang, Ying and Zhang, Kang and Kang, Jian and Zhang, Yong and Quan, Fusheng
Journal: Frontiers in cell and developmental biology (2021): 747722
Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas.
Authors: Ramesh, Kirti and Hu, Marian Y and Melzner, Frank and Bleich, Markus and Himmerkus, Nina
Journal: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology (2020): 691-700
Authors: Ramesh, Kirti and Hu, Marian Y and Melzner, Frank and Bleich, Markus and Himmerkus, Nina
Journal: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology (2020): 691-700
Altered Degranulation and pH of Neutrophil Phagosomes Impacts Antimicrobial Efficiency in Cystic Fibrosis.
Authors: Hayes, Elaine and Murphy, Mark P and Pohl, Kerstin and Browne, Niall and McQuillan, Karen and Saw, Le Er and Foley, Clare and Gargoum, Fatma and McElvaney, Oliver J and Hawkins, Padraig and Gunaratnam, Cedric and McElvaney, Noel G and Reeves, Emer P
Journal: Frontiers in immunology (2020): 600033
Authors: Hayes, Elaine and Murphy, Mark P and Pohl, Kerstin and Browne, Niall and McQuillan, Karen and Saw, Le Er and Foley, Clare and Gargoum, Fatma and McElvaney, Oliver J and Hawkins, Padraig and Gunaratnam, Cedric and McElvaney, Noel G and Reeves, Emer P
Journal: Frontiers in immunology (2020): 600033
Importance of measuring endolysosome, cytosolic, and extracellular pH in understanding the pathogenesis of and possible treatments for glioblastoma multiforme.
Authors: Halcrow, Peter and Khan, Nabab and Datta, Gaurav and Ohm, Joyce E and Chen, Xuesong and Geiger, Jonathan D
Journal: Cancer reports (2019)
Authors: Halcrow, Peter and Khan, Nabab and Datta, Gaurav and Ohm, Joyce E and Chen, Xuesong and Geiger, Jonathan D
Journal: Cancer reports (2019)
Levetiracetam mediates subtle pH-shifts in adult human neocortical pyramidal cells via an inhibition of the bicarbonate-driven neuronal pH-regulation - Implications for excitability and plasticity modulation.
Authors: Bonnet, Udo and Bingmann, Dieter and Speckmann, Erwin-Josef and Wiemann, Martin
Journal: Brain research (2019): 146-156
Authors: Bonnet, Udo and Bingmann, Dieter and Speckmann, Erwin-Josef and Wiemann, Martin
Journal: Brain research (2019): 146-156
Inhibitory Mechanisms of DHA/CQ on pH and Iron Homeostasis of Erythrocytic Stage Growth of Plasmodium Falciparum.
Authors: Tang, Tian and Xu, Wenhui and Ma, Ji and Wang, Huajing and Cui, Zhao and Jiang, Tingliang and Li, Canghai
Journal: Molecules (Basel, Switzerland) (2019)
Authors: Tang, Tian and Xu, Wenhui and Ma, Ji and Wang, Huajing and Cui, Zhao and Jiang, Tingliang and Li, Canghai
Journal: Molecules (Basel, Switzerland) (2019)
A Reliable and Non-destructive Method for Monitoring the Stromal pH in Isolated Chloroplasts Using a Fluorescent pH Probe.
Authors: Su, Pai-Hsiang and Lai, Yen-Hsun
Journal: Frontiers in plant science (2017): 2079
Authors: Su, Pai-Hsiang and Lai, Yen-Hsun
Journal: Frontiers in plant science (2017): 2079
pH buffering of single rat skeletal muscle fibers in the in vivo environment.
Authors: Tanaka, Yoshinori and Inagaki, Tadakatsu and Poole, David C and Kano, Yutaka
Journal: American journal of physiology. Regulatory, integrative and comparative physiology (2016): R926-33
Authors: Tanaka, Yoshinori and Inagaki, Tadakatsu and Poole, David C and Kano, Yutaka
Journal: American journal of physiology. Regulatory, integrative and comparative physiology (2016): R926-33
Alkaline Cytosolic pH and High Sodium Hydrogen Exchanger 1 (NHE1) Activity in Th9 Cells.
Authors: Singh, Yogesh and Zhou, Yuetao and Shi, Xiaolong and Zhang, Shaqiu and Umbach, Anja T and Salker, Madhuri S and Lang, Karl S and Lang, Florian
Journal: The Journal of biological chemistry (2016): 23662-23671
Authors: Singh, Yogesh and Zhou, Yuetao and Shi, Xiaolong and Zhang, Shaqiu and Umbach, Anja T and Salker, Madhuri S and Lang, Karl S and Lang, Florian
Journal: The Journal of biological chemistry (2016): 23662-23671
Application notes
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Cell Preparation Considerations and Troubleshooting
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Cell Preparation Considerations and Troubleshooting
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
How to measure intracellular pH using flow cytometry?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
What are the disadvantages of Nanodrop light spectrophotometer?
What are the advantages of a Nanodrop light spectrophotometer?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
What are the disadvantages of Nanodrop light spectrophotometer?
What are the advantages of a Nanodrop light spectrophotometer?
AssayWise
Intracellular pH Measurement with Dual Excitation Fluorescence Sensor BCFL
RATIOWORKS pH 165, AM: A Dual Excitation/Emission Fluorescence Probe For Imaging Live Cells
Investigating Intercellular Channels: Focus on Gap Junctions
Nucleic Acid Detection, Quantification and Imaging
Cal-520® AM, a Development Overview
RATIOWORKS pH 165, AM: A Dual Excitation/Emission Fluorescence Probe For Imaging Live Cells
Investigating Intercellular Channels: Focus on Gap Junctions
Nucleic Acid Detection, Quantification and Imaging
Cal-520® AM, a Development Overview