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Protonex™ Red 780 AM *Cell-Permeable*

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Physical properties
Molecular weight798.89
Spectral properties
Excitation (nm)748
Emission (nm)769
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Protonex™ Red 780 AM is the cell-permeable version of Protonex™ Red 780. Protonex™ Red 780 dye works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 780 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 780 is bound to an acidic intracellular target, it becomes highly fluorescent and emits near-infrared light when excited by a red laser. By measuring the fluorescence intensity of Protonex™ Red 780, one can label or monitor the acidic intracellular targets in live cells.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Red 780 AM *Cell-Permeable* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM125.174 µL625.868 µL1.252 mL6.259 mL12.517 mL
5 mM25.035 µL125.174 µL250.347 µL1.252 mL2.503 mL
10 mM12.517 µL62.587 µL125.174 µL625.868 µL1.252 mL

Molarity calculator

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Spectral properties

Excitation (nm)748
Emission (nm)769

Product Family

NameExcitation (nm)Emission (nm)
Protonex™ Red 670 AM *Cell-Permeable*643660
Protonex™ Red 780 acid748769


View all 50 references: Citation Explorer
mOrange2, a Genetically Encoded, pH Sensitive Fluorescent Protein, is an Alternative to BCECF-AM to Measure Intracellular pH to Determine NHE3 and DRA Activity.
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Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2022): 39-49
Effects of Diluent pH on Enrichment and Performance of Dairy Goat X/Y Sperm.
Authors: He, Qifu and Wu, Shenghui and Huang, Ming and Wang, Ying and Zhang, Kang and Kang, Jian and Zhang, Yong and Quan, Fusheng
Journal: Frontiers in cell and developmental biology (2021): 747722
Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas.
Authors: Ramesh, Kirti and Hu, Marian Y and Melzner, Frank and Bleich, Markus and Himmerkus, Nina
Journal: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology (2020): 691-700
Altered Degranulation and pH of Neutrophil Phagosomes Impacts Antimicrobial Efficiency in Cystic Fibrosis.
Authors: Hayes, Elaine and Murphy, Mark P and Pohl, Kerstin and Browne, Niall and McQuillan, Karen and Saw, Le Er and Foley, Clare and Gargoum, Fatma and McElvaney, Oliver J and Hawkins, Padraig and Gunaratnam, Cedric and McElvaney, Noel G and Reeves, Emer P
Journal: Frontiers in immunology (2020): 600033
Importance of measuring endolysosome, cytosolic, and extracellular pH in understanding the pathogenesis of and possible treatments for glioblastoma multiforme.
Authors: Halcrow, Peter and Khan, Nabab and Datta, Gaurav and Ohm, Joyce E and Chen, Xuesong and Geiger, Jonathan D
Journal: Cancer reports (2019)
Levetiracetam mediates subtle pH-shifts in adult human neocortical pyramidal cells via an inhibition of the bicarbonate-driven neuronal pH-regulation - Implications for excitability and plasticity modulation.
Authors: Bonnet, Udo and Bingmann, Dieter and Speckmann, Erwin-Josef and Wiemann, Martin
Journal: Brain research (2019): 146-156
Inhibitory Mechanisms of DHA/CQ on pH and Iron Homeostasis of Erythrocytic Stage Growth of Plasmodium Falciparum.
Authors: Tang, Tian and Xu, Wenhui and Ma, Ji and Wang, Huajing and Cui, Zhao and Jiang, Tingliang and Li, Canghai
Journal: Molecules (Basel, Switzerland) (2019)
A Reliable and Non-destructive Method for Monitoring the Stromal pH in Isolated Chloroplasts Using a Fluorescent pH Probe.
Authors: Su, Pai-Hsiang and Lai, Yen-Hsun
Journal: Frontiers in plant science (2017): 2079
pH buffering of single rat skeletal muscle fibers in the in vivo environment.
Authors: Tanaka, Yoshinori and Inagaki, Tadakatsu and Poole, David C and Kano, Yutaka
Journal: American journal of physiology. Regulatory, integrative and comparative physiology (2016): R926-33
Different rate-limiting activities of intracellular pH regulators for HCO3- secretion stimulated by forskolin and carbachol in rat parotid intralobular ducts.
Authors: Ueno, Kaori and Hirono, Chikara and Kitagawa, Michinori and Shiba, Yoshiki and Sugita, Makoto
Journal: The journal of physiological sciences : JPS (2016): 477-490