AAT Bioquest


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ShippingStandard overnight for United States, inquire for international
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Physical properties
Molecular weight437.30
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


See also: Nucleus
Molecular weight
PUR-1 is essentially non-fluorescent in the absence of nucleic acids. Its fluorescence is significantly enhanced in the presence of DNA and/or RNA. In the presence of RNA, PUR-1 has fluorescence at ~478 nm with fluorescence quantum yield of ~40%. PUR-1 dye is excitable at 488 nm (with maximal excitation at 460 nm) and emits fluorescence between 470 to 550 nm with a maximum emission at 478 nm in the presence of nucleic acids. The dye can be used for analyzing blood-borne parasite infections in combination with a flow cytometer.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of PUR-1 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM228.676 µL1.143 mL2.287 mL11.434 mL22.868 mL
5 mM45.735 µL228.676 µL457.352 µL2.287 mL4.574 mL
10 mM22.868 µL114.338 µL228.676 µL1.143 mL2.287 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



View all 52 references: Citation Explorer
Inhibition of Mitochondrial Fission Protein Reduced Mechanical Allodynia and Suppressed Spinal Mitochondrial Superoxide Induced by Perineural Human Immunodeficiency Virus gp120 in Rats
Authors: K, undefined and a H, Liu S, Iida T, Yi H, Huang W, Levitt RC, Lubarsky DA, C and iotti KA, Hao S.
Journal: Anesth Analg (2016): 264
Up-Regulation of Mitochondrial Antioxidant Superoxide Dismutase Underpins Persistent Cardiac Nutritional-Preconditioning by Long Chain n-3 Polyunsaturated Fatty Acids in the Rat
Authors: Abdukeyum GG, Owen AJ, Larkin TA, McLennan PL.
Journal: J Clin Med (2016): 32
Mitochondrial flashes: dump superoxide and dance with protons now
Authors: Demaurex N, Schwarzl and er M., undefined
Journal: Antioxid Redox Signal. (2016)
Expression and polymorphism (rs4880) of mitochondrial superoxide dismutase (SOD2) and asparaginase induced hepatotoxicity in adult patients with acute lymphoblastic leukemia
Authors: Alachkar H, Fulton N, Sanford B, Malnassy G, Mutonga M, Larson RA, Bloomfield CD, Marcucci G, Nakamura Y, Stock W.
Journal: Pharmacogenomics J. (2016)
Adipocyte-Specific Deletion of Manganese Superoxide Dismutase Protects From Diet-Induced Obesity Through Increased Mitochondrial Uncoupling and Biogenesis
Authors: Han YH, Buffolo M, Pires KM, Pei S, Scherer PE, Boudina S.
Journal: Diabetes (2016): 2639
Mitochondrial generation of superoxide and hydrogen peroxide as the source of mitochondrial redox signaling
Authors: Br, undefined and MD., undefined
Journal: Free Radic Biol Med. (2016)
Near-Infrared Fluorescence Probe for in Situ Detection of Superoxide Anion and Hydrogen Polysulfides in Mitochondrial Oxidative Stress
Authors: Huang Y, Yu F, Wang J, Chen L.
Journal: Anal Chem (2016): 4122
Potential role of mitochondrial superoxide decreasing ferrochelatase and heme in coronary artery soluble guanylate cyclase depletion by angiotensin II
Authors: Patel D, Alhawaj R, Kelly MR, Accarino JJ, Lakhkar A, Gupte SA, Sun D, Wolin MS.
Journal: Am J Physiol Heart Circ Physiol (2016): H1439
The study of the mechanism of arsenite toxicity in respiration-deficient cells reveals that NADPH oxidase-derived superoxide promotes the same downstream events mediated by mitochondrial superoxide in respiration-proficient cells
Authors: Guidarelli A, Fiorani M, Carloni S, Cerioni L, Balduini W, Cantoni O.
Journal: Toxicol Appl Pharmacol (2016): 35
20-HETE-induced mitochondrial superoxide production and inflammatory phenotype in vascular smooth muscle is prevented by glucose-6-phosphate dehydrogenase inhibition
Authors: Lakhkar A, Dhagia V, Joshi SR, Gotlinger K, Patel D, Sun D, Wolin MS, Schwartzman ML, Gupte SA.
Journal: Am J Physiol Heart Circ Physiol (2016): H1107