PUR-1
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 437.30 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H340 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Overview | SDSProtocol |
See also: Nucleus
Molecular weight 437.30 |
PUR-1 is essentially non-fluorescent in the absence of nucleic acids. Its fluorescence is significantly enhanced in the presence of DNA and/or RNA. In the presence of RNA, PUR-1 has fluorescence at ~478 nm with fluorescence quantum yield of ~40%. PUR-1 dye is excitable at 488 nm (with maximal excitation at 460 nm) and emits fluorescence between 470 to 550 nm with a maximum emission at 478 nm in the presence of nucleic acids. The dye can be used for analyzing blood-borne parasite infections in combination with a flow cytometer.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of PUR-1 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 228.676 µL | 1.143 mL | 2.287 mL | 11.434 mL | 22.868 mL |
5 mM | 45.735 µL | 228.676 µL | 457.352 µL | 2.287 mL | 4.574 mL |
10 mM | 22.868 µL | 114.338 µL | 228.676 µL | 1.143 mL | 2.287 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
Oxazine 1 *CAS 24796-94-9* | 648 | 668 | 123000 | 0.11001 |
DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 | 0.341 |
DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* | 491 | 508 | 989001 | 0.521 |
JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 | - |
BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] | 349 | 421 | - | - |
DiYO™-1 [equivalent to YOYO®-1] | 491 | 508 | 989001 | 0.521 |
DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* | 433 | 457 | 920001 | 0.61 |
EthD-1 [Ethidium Homodimer-1] | 528 | 617 | - | - |
References
View all 52 references: Citation Explorer
Activation of Mitochondrial Uncoupling Protein 4 and ATP-Sensitive Potassium Channel Cumulatively Decreases Superoxide Production in Insect Mitochondria
Authors: Slocinska M, Rosinski G, Jarmuszkiewicz W.
Journal: Protein Pept Lett (2016): 63
Authors: Slocinska M, Rosinski G, Jarmuszkiewicz W.
Journal: Protein Pept Lett (2016): 63
Rasagiline prevents cyclosporine A-sensitive superoxide flashes induced by PK11195, the initial signal of mitochondrial membrane permeabilization and apoptosis
Authors: Wu Y, Shamoto-Nagai M, Maruyama W, Osawa T, Naoi M.
Journal: J Neural Transm (Vienna) (2016): 491
Authors: Wu Y, Shamoto-Nagai M, Maruyama W, Osawa T, Naoi M.
Journal: J Neural Transm (Vienna) (2016): 491
Mitochondrial disease-related mutations at the cytochrome b-iron-sulfur protein (ISP) interface: Molecular effects on the large-scale motion of ISP and superoxide generation studied in Rhodobacter capsulatus cytochrome bc1
Authors: Ekiert R, Borek A, Kuleta P, Czernek J, Osyczka A.
Journal: Biochim Biophys Acta (2016): 1102
Authors: Ekiert R, Borek A, Kuleta P, Czernek J, Osyczka A.
Journal: Biochim Biophys Acta (2016): 1102
Oleuropein Prevents Neuronal Death, Mitigates Mitochondrial Superoxide Production and Modulates Autophagy in a Dopaminergic Cellular Model
Authors: Achour I, Arel-Dubeau AM, Renaud J, Legr and M, Attard E, Germain M, Martinoli MG.
Journal: Int J Mol Sci (2016): 1293
Authors: Achour I, Arel-Dubeau AM, Renaud J, Legr and M, Attard E, Germain M, Martinoli MG.
Journal: Int J Mol Sci (2016): 1293
Manganese-superoxide dismutase (Mn-SOD) overexpression is a common event in colorectal cancers with mitochondrial microsatellite instability
Authors: Govatati S, Malempati S, Saradamma B, Divyamaanasa D, Naidu BP, Bramhachari PV, Narayana N, Shivaji S, Bhanoori M, Tamanam RR, Rao PS, Nallanchakravarthula V.
Journal: Tumour Biol. (2016)
Authors: Govatati S, Malempati S, Saradamma B, Divyamaanasa D, Naidu BP, Bramhachari PV, Narayana N, Shivaji S, Bhanoori M, Tamanam RR, Rao PS, Nallanchakravarthula V.
Journal: Tumour Biol. (2016)
Production of superoxide/hydrogen peroxide by the mitochondrial 2-oxoadipate dehydrogenase complex
Authors: Goncalves RL, Bunik VI, Br and MD., undefined
Journal: Free Radic Biol Med (2016): 247
Authors: Goncalves RL, Bunik VI, Br and MD., undefined
Journal: Free Radic Biol Med (2016): 247
A Unifying Mechanism for Mitochondrial Superoxide Production during Ischemia-Reperfusion Injury
Authors: Chouchani ET, Pell VR, James AM, Work LM, Saeb-Parsy K, Frezza C, Krieg T, Murphy MP.
Journal: Cell Metab (2016): 254
Authors: Chouchani ET, Pell VR, James AM, Work LM, Saeb-Parsy K, Frezza C, Krieg T, Murphy MP.
Journal: Cell Metab (2016): 254
Rotenone-stimulated superoxide release from mitochondrial complex I acutely augments L-type Ca2+ current in A7r5 aortic smooth muscle cells
Authors: Ochi R, Dhagia V, Lakhkar A, Patel D, Wolin MS, Gupte SA.
Journal: Am J Physiol Heart Circ Physiol (2016): H1118
Authors: Ochi R, Dhagia V, Lakhkar A, Patel D, Wolin MS, Gupte SA.
Journal: Am J Physiol Heart Circ Physiol (2016): H1118
Recombinant Mitochondrial Manganese Containing Superoxide Dismutase Protects Against Ochratoxin A-Induced Nephrotoxicity
Authors: Ciarcia R, Damiano S, Squillacioti C, Mirabella N, Pagnini U, Florio A, Severino L, Capasso G, Borrelli A, Mancini A, Boffo S, Romano G, Giordano A, Florio S.
Journal: J Cell Biochem (2016): 1352
Authors: Ciarcia R, Damiano S, Squillacioti C, Mirabella N, Pagnini U, Florio A, Severino L, Capasso G, Borrelli A, Mancini A, Boffo S, Romano G, Giordano A, Florio S.
Journal: J Cell Biochem (2016): 1352
Mitochondrial Calpain-1 Disrupts ATP Synthase and Induces Superoxide Generation in Type 1 Diabetic Hearts: A Novel Mechanism Contributing to Diabetic Cardiomyopathy
Authors: Ni R, Zheng D, Xiong S, Hill DJ, Sun T, Gardiner RB, Fan GC, Lu Y, Abel ED, Greer PA, Peng T.
Journal: Diabetes (2016): 255
Authors: Ni R, Zheng D, Xiong S, Hill DJ, Sun T, Gardiner RB, Fan GC, Lu Y, Abel ED, Greer PA, Peng T.
Journal: Diabetes (2016): 255
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?