PUR-1
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 437.30 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H340 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R68 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 41116134 |
| Overview |  SDSProtocol |
See also: Nucleus
Molecular weight 437.30 |
PUR-1 is essentially non-fluorescent in the absence of nucleic acids. Its fluorescence is significantly enhanced in the presence of DNA and/or RNA. In the presence of RNA, PUR-1 has fluorescence at ~478 nm with fluorescence quantum yield of ~40%. PUR-1 dye is excitable at 488 nm (with maximal excitation at 460 nm) and emits fluorescence between 470 to 550 nm with a maximum emission at 478 nm in the presence of nucleic acids. The dye can be used for analyzing blood-borne parasite infections in combination with a flow cytometer.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of PUR-1 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 228.676 µL | 1.143 mL | 2.287 mL | 11.434 mL | 22.868 mL |
| 5 mM | 45.735 µL | 228.676 µL | 457.352 µL | 2.287 mL | 4.574 mL |
| 10 mM | 22.868 µL | 114.338 µL | 228.676 µL | 1.143 mL | 2.287 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Oxazine 1 *CAS 24796-94-9* | 648 | 668 | 123000 | 0.11001 |
| DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 | 0.341 |
| DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* | 491 | 508 | 989001 | 0.521 |
| JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 | - |
| BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] | 349 | 421 | - | - |
| DiYO™-1 [equivalent to YOYO®-1] | 491 | 508 | 989001 | 0.521 |
| DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* | 433 | 457 | 920001 | 0.61 |
Images
Figure 1. Chemical structure for PUR-1
References
View all 52 references: Citation Explorer
Activation of Mitochondrial Uncoupling Protein 4 and ATP-Sensitive Potassium Channel Cumulatively Decreases Superoxide Production in Insect Mitochondria
Authors: Slocinska M, Rosinski G, Jarmuszkiewicz W.
Journal: Protein Pept Lett (2016): 63
Authors: Slocinska M, Rosinski G, Jarmuszkiewicz W.
Journal: Protein Pept Lett (2016): 63
Rasagiline prevents cyclosporine A-sensitive superoxide flashes induced by PK11195, the initial signal of mitochondrial membrane permeabilization and apoptosis
Authors: Wu Y, Shamoto-Nagai M, Maruyama W, Osawa T, Naoi M.
Journal: J Neural Transm (Vienna) (2016): 491
Authors: Wu Y, Shamoto-Nagai M, Maruyama W, Osawa T, Naoi M.
Journal: J Neural Transm (Vienna) (2016): 491
Mitochondrial disease-related mutations at the cytochrome b-iron-sulfur protein (ISP) interface: Molecular effects on the large-scale motion of ISP and superoxide generation studied in Rhodobacter capsulatus cytochrome bc1
Authors: Ekiert R, Borek A, Kuleta P, Czernek J, Osyczka A.
Journal: Biochim Biophys Acta (2016): 1102
Authors: Ekiert R, Borek A, Kuleta P, Czernek J, Osyczka A.
Journal: Biochim Biophys Acta (2016): 1102
Oleuropein Prevents Neuronal Death, Mitigates Mitochondrial Superoxide Production and Modulates Autophagy in a Dopaminergic Cellular Model
Authors: Achour I, Arel-Dubeau AM, Renaud J, Legr and M, Attard E, Germain M, Martinoli MG.
Journal: Int J Mol Sci (2016): 1293
Authors: Achour I, Arel-Dubeau AM, Renaud J, Legr and M, Attard E, Germain M, Martinoli MG.
Journal: Int J Mol Sci (2016): 1293
Manganese-superoxide dismutase (Mn-SOD) overexpression is a common event in colorectal cancers with mitochondrial microsatellite instability
Authors: Govatati S, Malempati S, Saradamma B, Divyamaanasa D, Naidu BP, Bramhachari PV, Narayana N, Shivaji S, Bhanoori M, Tamanam RR, Rao PS, Nallanchakravarthula V.
Journal: Tumour Biol. (2016)
Authors: Govatati S, Malempati S, Saradamma B, Divyamaanasa D, Naidu BP, Bramhachari PV, Narayana N, Shivaji S, Bhanoori M, Tamanam RR, Rao PS, Nallanchakravarthula V.
Journal: Tumour Biol. (2016)
Production of superoxide/hydrogen peroxide by the mitochondrial 2-oxoadipate dehydrogenase complex
Authors: Goncalves RL, Bunik VI, Br and MD., undefined
Journal: Free Radic Biol Med (2016): 247
Authors: Goncalves RL, Bunik VI, Br and MD., undefined
Journal: Free Radic Biol Med (2016): 247
A Unifying Mechanism for Mitochondrial Superoxide Production during Ischemia-Reperfusion Injury
Authors: Chouchani ET, Pell VR, James AM, Work LM, Saeb-Parsy K, Frezza C, Krieg T, Murphy MP.
Journal: Cell Metab (2016): 254
Authors: Chouchani ET, Pell VR, James AM, Work LM, Saeb-Parsy K, Frezza C, Krieg T, Murphy MP.
Journal: Cell Metab (2016): 254
Rotenone-stimulated superoxide release from mitochondrial complex I acutely augments L-type Ca2+ current in A7r5 aortic smooth muscle cells
Authors: Ochi R, Dhagia V, Lakhkar A, Patel D, Wolin MS, Gupte SA.
Journal: Am J Physiol Heart Circ Physiol (2016): H1118
Authors: Ochi R, Dhagia V, Lakhkar A, Patel D, Wolin MS, Gupte SA.
Journal: Am J Physiol Heart Circ Physiol (2016): H1118
Recombinant Mitochondrial Manganese Containing Superoxide Dismutase Protects Against Ochratoxin A-Induced Nephrotoxicity
Authors: Ciarcia R, Damiano S, Squillacioti C, Mirabella N, Pagnini U, Florio A, Severino L, Capasso G, Borrelli A, Mancini A, Boffo S, Romano G, Giordano A, Florio S.
Journal: J Cell Biochem (2016): 1352
Authors: Ciarcia R, Damiano S, Squillacioti C, Mirabella N, Pagnini U, Florio A, Severino L, Capasso G, Borrelli A, Mancini A, Boffo S, Romano G, Giordano A, Florio S.
Journal: J Cell Biochem (2016): 1352
Mitochondrial Calpain-1 Disrupts ATP Synthase and Induces Superoxide Generation in Type 1 Diabetic Hearts: A Novel Mechanism Contributing to Diabetic Cardiomyopathy
Authors: Ni R, Zheng D, Xiong S, Hill DJ, Sun T, Gardiner RB, Fan GC, Lu Y, Abel ED, Greer PA, Peng T.
Journal: Diabetes (2016): 255
Authors: Ni R, Zheng D, Xiong S, Hill DJ, Sun T, Gardiner RB, Fan GC, Lu Y, Abel ED, Greer PA, Peng T.
Journal: Diabetes (2016): 255