RatioWorks™ PDMPO
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 366.41 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 333 |
Emission (nm) | 531 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Overview | ![]() ![]() |
See also: Intracellular pH, Physiological Probes
Molecular weight 366.41 | Excitation (nm) 333 | Emission (nm) 531 |
The existing pH probes are ill-adapted to study acidic organelles such as lysosomes, endosomes, spermatozoa and acrosomes because their fluorescence is significantly reduced at lower pH. In addition, most of the existing pH probes (such as BCECF and SNARF) are not selectively localized in acidic organelles. The growing potential of ratio imaging is significantly limited by the lack of appropriate fluorescent probes for acidic organelles although ratio imaging has received intensive attention in the past few decades. PDMPO [2-(4-pyridyl)-5-((4-(2-dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole] is the same molecule to LysoSensor Yellow/Blue, and characterized as acidotropic dual-excitation and dual-emission pH probe. It emits intense yellow fluorescence at lower pH and gives intense blue fluorescence at higher pH. This unique pH-dependent fluorescence makes PDMPO an ideal pH probe for acidic organelles with pKa = 4.47. PDMPO selectively labels acidic organelles (such as lysosomes) of live cells and the two distinct emission peaks can be used to monitor the pH fluctuations of live cells in ratio measurements. Additionally, the very large Stokes shift and excellent photostability of PDMPO make PDMPO an excellent fluorescent acidotropic reagent for fluorescence imaging. The unique fluorescence properties of PDMPO might give researchers a new tool with which to study acidic organelles of live cells. PDMPO can be well excited by the violet laser at 405 nm for flow cytometric applications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of RatioWorks™ PDMPO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 272.918 µL | 1.365 mL | 2.729 mL | 13.646 mL | 27.292 mL |
5 mM | 54.584 µL | 272.918 µL | 545.837 µL | 2.729 mL | 5.458 mL |
10 mM | 27.292 µL | 136.459 µL | 272.918 µL | 1.365 mL | 2.729 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Citations
View all 6 citations: Citation Explorer
Mannose ameliorates experimental colitis by protecting intestinal barrier integrity
Authors: Dong, Lijun and Xie, Jingwen and Wang, Youyi and Jiang, Honglian and Chen, Kai and Li, Dantong and Wang, Jing and Liu, Yunzhi and He, Jia and Zhou, Jia and others,
Journal: Nature communications (2022): 1--17
Authors: Dong, Lijun and Xie, Jingwen and Wang, Youyi and Jiang, Honglian and Chen, Kai and Li, Dantong and Wang, Jing and Liu, Yunzhi and He, Jia and Zhou, Jia and others,
Journal: Nature communications (2022): 1--17
Mycobacterium tuberculosis Utilizes Host Histamine Receptor H1 to Modulate Reactive Oxygen Species Production and Phagosome Maturation via the p38MAPK-NOX2 Axis
Authors: Mo, Siwei and Guo, Jiubiao and Ye, Taosheng and Zhang, Ximeng and Zeng, Jiang and Xu, Yuzhong and Peng, Bin and Dai, Youchao and Xiao, Wei and Zhang, Peize and others,
Journal: Mbio (2022): e02004--22
Authors: Mo, Siwei and Guo, Jiubiao and Ye, Taosheng and Zhang, Ximeng and Zeng, Jiang and Xu, Yuzhong and Peng, Bin and Dai, Youchao and Xiao, Wei and Zhang, Peize and others,
Journal: Mbio (2022): e02004--22
The molecular basis for pore pattern morphogenesis in diatom silica
Authors: Heintze, Christoph and Babenko, Iaroslav and Suchanova, Jirina Zackova and Skeffington, Alastair and Friedrich, Benjamin and Kroeger, Nils
Journal: (2022)
Authors: Heintze, Christoph and Babenko, Iaroslav and Suchanova, Jirina Zackova and Skeffington, Alastair and Friedrich, Benjamin and Kroeger, Nils
Journal: (2022)
An intimate view into the silica deposition vesicles of diatoms
Authors: Heintze, Christoph and Formanek, Petr and Pohl, Darius and Hauptstein, Jannes and Rellinghaus, Bernd and Kr{\"o}ger, Nils
Journal: BMC Materials (2020): 1--15
Authors: Heintze, Christoph and Formanek, Petr and Pohl, Darius and Hauptstein, Jannes and Rellinghaus, Bernd and Kr{\"o}ger, Nils
Journal: BMC Materials (2020): 1--15
A New View into the Silica Deposition Vesicles of Diatoms
Authors: Kr{\"o}ger, N and Heintze, C and Formanek, P and Pohl, D and Hauptstein, J and Rellinghaus, B
Journal: (2020)
Authors: Kr{\"o}ger, N and Heintze, C and Formanek, P and Pohl, D and Hauptstein, J and Rellinghaus, B
Journal: (2020)
iTRAQ-based proteomic analysis of the metabolism mechanism associated with silicon response in the marine diatom Thalassiosira pseudonana
Authors: Du, Chao and Liang, Jun-Rong and Chen, Dan-Dan and Xu, Bin and Zhuo, Wen-Hao and Gao, Ya-Hui and Chen, Chang-Ping and Bowler, Chris and Zhang, Wen
Journal: Journal of proteome research (2014): 720--734
Authors: Du, Chao and Liang, Jun-Rong and Chen, Dan-Dan and Xu, Bin and Zhuo, Wen-Hao and Gao, Ya-Hui and Chen, Chang-Ping and Bowler, Chris and Zhang, Wen
Journal: Journal of proteome research (2014): 720--734
References
View all 8 references: Citation Explorer
Using LysoSensor Yellow/Blue DND-160 to sense acidic pH under high hydrostatic pressures
Authors: DePedro HM, Urayama P.
Journal: Anal Biochem (2009): 359
Authors: DePedro HM, Urayama P.
Journal: Anal Biochem (2009): 359
Acidic microclimate pH distribution in PLGA microspheres monitored by confocal laser scanning microscopy
Authors: Ding AG, Schwendeman SP.
Journal: Pharm Res (2008): 2041
Authors: Ding AG, Schwendeman SP.
Journal: Pharm Res (2008): 2041
Heterogeneity of acid secretion induced by carbachol and histamine along the gastric gland axis and its relationship to [Ca2+]i
Authors: Perez-Zoghbi JF, Mayora A, Ruiz MC, Michelangeli F.
Journal: Am J Physiol Gastrointest Liver Physiol (2008): G671
Authors: Perez-Zoghbi JF, Mayora A, Ruiz MC, Michelangeli F.
Journal: Am J Physiol Gastrointest Liver Physiol (2008): G671
Platelet-activating factor stimulates cytoplasmic alkalinization and granule acidification in human eosinophils
Authors: Bankers-Fulbright JL, Kephart GM, Bartemes KR, Kita H, O'Grady SM.
Journal: J Cell Sci (2004): 5749
Authors: Bankers-Fulbright JL, Kephart GM, Bartemes KR, Kita H, O'Grady SM.
Journal: J Cell Sci (2004): 5749
Divalent cations regulate acidity within the lumen and tubulovesicle compartment of gastric parietal cells
Authors: Gerbino A, Hofer AM, McKay B, Lau BW, Soybel DI.
Journal: Gastroenterology (2004): 182
Authors: Gerbino A, Hofer AM, McKay B, Lau BW, Soybel DI.
Journal: Gastroenterology (2004): 182
Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter
Authors: Schroder HC, Perovic-Ottstadt S, Rothenberger M, Wiens M, Schwertner H, Batel R, Korzhev M, Muller IM, Muller WE.
Journal: Biochem J (2004): 665
Authors: Schroder HC, Perovic-Ottstadt S, Rothenberger M, Wiens M, Schwertner H, Batel R, Korzhev M, Muller IM, Muller WE.
Journal: Biochem J (2004): 665
Elevated lysosomal pH in neuronal ceroid lipofuscinoses (NCLs)
Authors: Holopainen JM, Saarikoski J, Kinnunen PK, Jarvela I.
Journal: Eur J Biochem (2001): 5851
Authors: Holopainen JM, Saarikoski J, Kinnunen PK, Jarvela I.
Journal: Eur J Biochem (2001): 5851
A novel fluorescent silica tracer for biological silicification studies
Authors: Shimizu K, Del Amo Y, Brzezinski MA, Stucky GD, Morse DE.
Journal: Chem Biol (2001): 1051
Authors: Shimizu K, Del Amo Y, Brzezinski MA, Stucky GD, Morse DE.
Journal: Chem Biol (2001): 1051
Application notes
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
FAQ
How to measure intracellular pH using flow cytometry?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
Are NADH and ROS related?
Are there any alternatives for ethidium bromide in agarose gels?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
Are NADH and ROS related?
Are there any alternatives for ethidium bromide in agarose gels?