RatioWorks™ PH165
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 517.54 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 463 |
| Emission (nm) | 641 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
Molecular weight 517.54 | Excitation (nm) 463 | Emission (nm) 641 |
RatioWorks™ PH165 is a novel fluorescent probe designed for pH measurement in solution, featuring pH-dependent fluorescence spectra and unique dual excitation and dual emission properties. At low pH, RatioWorks™ PH165 is weakly fluorescent in the far-red spectrum region and highly fluorescent in the red spectrum region. As pH increases, it shows higher fluorescence in the far-red spectrum and lower fluorescence in the red spectrum. This probe quantifies pH in the 4 to 9 range, utilizing dual excitation/emission at 497/594 nm and 578/654 nm, respectively, and has a pKa of ~7.1. The red fluorescence spectrum of RatioWorks™ PH165 makes it suitable for multiplexing tests with green fluorophores like GFP. RatioWorks™ PH165 AM is cell-permeable and compatible with platforms such as fluorescence microscopy and microplate readers.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of RatioWorks™ PH165 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 193.222 µL | 966.109 µL | 1.932 mL | 9.661 mL | 19.322 mL |
| 5 mM | 38.644 µL | 193.222 µL | 386.444 µL | 1.932 mL | 3.864 mL |
| 10 mM | 19.322 µL | 96.611 µL | 193.222 µL | 966.109 µL | 1.932 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Images
Figure 1. The pH dependent absorbance spectra of RatioWorks™ PH165.
Figure 2. The pH dependent Emission spectra of RatioWorks™ PH165.
Figure 3. The pH dependent Excitation spectra of RatioWorks™ PH165.
Citations
View all 3 citations: Citation Explorer
Intracellular pH Measurements in Glioblastoma Cells Using the pH-Sensitive Dye BCECF
Authors: Galifianakis, N. V., Placantonakis, D. G., Chesler, M.
Journal: Methods Mol Biol (2018): 103-109
Authors: Galifianakis, N. V., Placantonakis, D. G., Chesler, M.
Journal: Methods Mol Biol (2018): 103-109
Evaluation of BCECF fluorescence ratio imaging to properly measure gastric intramucosal pH variations in vivo
Authors: Rochon, P., Jourdain, M., Mangalaboyi, J., Fourrier, F., Soulie-Begu, S., Buys, B., Dehlin, G., Lesage, J. C., Chambrin, M. C., Mordon, S.
Journal: J Biomed Opt (2007): 064014
Authors: Rochon, P., Jourdain, M., Mangalaboyi, J., Fourrier, F., Soulie-Begu, S., Buys, B., Dehlin, G., Lesage, J. C., Chambrin, M. C., Mordon, S.
Journal: J Biomed Opt (2007): 064014
Calibration methods and avoidance of errors in measurement of intracellular pH (pHcyt) using the indicator bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in human platelets
Authors: Valant, P. A., Haynes, D. H.
Journal: J Fluoresc (1992): 191-206
Authors: Valant, P. A., Haynes, D. H.
Journal: J Fluoresc (1992): 191-206
References
View all 1 references: Citation Explorer
Estimation of intramitochondrial pca and ph by fura-2 and 2,7 biscarboxyethyl-5(6)-carboxyfluorescein (bcecf) fluorescence
Authors: Davis MH, Altschuld RA, Jung DW, Brierley GP.
Journal: Biochem Biophys Res Commun (1987): 40
Authors: Davis MH, Altschuld RA, Jung DW, Brierley GP.
Journal: Biochem Biophys Res Commun (1987): 40