Name: RatioWorks™ PH165
Brand: AAT Bioquest
SKU: 21213
Price: 165 USD
Availability: InStock

RatioWorks™ PH165

RatioWorks™ PH165 is a novel fluorescent probe designed for pH measurement in solution, featuring pH-dependent fluorescence spectra and unique dual excitation and dual emission properties. At low pH, RatioWorks™ PH165 is weakly fluorescent in the far-red spectrum region and highly fluorescent in the red spectrum region. As pH increases, it shows higher fluorescence in the far-red spectrum and lower fluorescence in the red spectrum. This probe quantifies pH in the 4 to 9 range, utilizing dual excitation/emission at 497/594 nm and 578/654 nm, respectively, and has a pKa of ~7.1. The red fluorescence spectrum of RatioWorks™ PH165 makes it suitable for multiplexing tests with green fluorophores like GFP. RatioWorks™ PH165 AM is cell-permeable and compatible with platforms such as fluorescence microscopy and microplate readers.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21213	10x50 ug	Price

Citations

View all 3 citations: Citation Explorer

Intracellular pH Measurements in Glioblastoma Cells Using the pH-Sensitive Dye BCECF

Authors:

Galifianakis, N. V., Placantonakis, D. G., Chesler, M.

Journal:

Methods Mol Biol (2018): 103-109

Evaluation of BCECF fluorescence ratio imaging to properly measure gastric intramucosal pH variations in vivo

Authors:

Rochon, P., Jourdain, M., Mangalaboyi, J., Fourrier, F., Soulie-Begu, S., Buys, B., Dehlin, G., Lesage, J. C., Chambrin, M. C., Mordon, S.

Journal:

J Biomed Opt (2007): 064014

Calibration methods and avoidance of errors in measurement of intracellular pH (pHcyt) using the indicator bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in human platelets

Authors:

Valant, P. A., Haynes, D. H.

Journal:

J Fluoresc (1992): 191-206

Physical properties

Molecular weight	517.54
Solvent	DMSO

Spectral properties

Excitation (nm)	463
Emission (nm)	641

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 6, 2025