RatioWorks™ BCFL Acid *Superior replacement for BCECF*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~500 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 504 |
Emission (nm) | 527 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Overview | ![]() ![]() |
See also: Intracellular pH, Physiological Probes
Molecular weight ~500 | Excitation (nm) 504 | Emission (nm) 527 |
Intracellular pH plays an important modulating role in many cellular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, cell adhesion and other cellular processes. pH-sensitive fluorescent dyes have been widely applied to monitor changes in intracellular pH in recent years. Imaging techniques that use fluorescent pH indicators also allow researchers to investigate these processes with much greater spatial resolution and sampling density that can be achieved using other technologies such as microelectrode. Among them, 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) is the most popular pH probe since it can be used to monitor cellular pH ratiometrically. However, all the commercial BCECF AM is a complex mixture of at least three isomers with different ratios from batch to batch, complicating the BCECF applications. BCFL is developed to overcome this isomer difficulty associated with BCECF AM. As BCECF, BCFL exhibits a pH-dependent dual excitation, essentially identical to BCECF. It has pKa of ~7.0, identical to BCECF too. As with BCECF, the dual excitation spectrum of BCFL with an isosbestic point at 454 nm should make BCFL a good excitation-ratiometrie pH indicator. BCFL ratiometric imaging makes intracellular pH determination essentially independent of several variable factors, including dye concentration, path length, cellular leakage and photobleaching rate. BCFL, AM is a single isomer, making the pH measurement much more reproducible than the BCECF, AM, which is consisted of quite a few different isomers. This BCFL is the esterase-hydrolyzed product of BCFL AM that can be used a reference standard with BCFL AM.
Product Family
Name | Excitation (nm) | Emission (nm) |
RatioWorks™ BCFL, AM *Superior replacement for BCECF* | 504 | 527 |
RatioWorks™ BCFL, SE | 504 | 527 |
Images
Citations
View all 2 citations: Citation Explorer
The F0F1 ATP synthase regulates human neutrophil migration through cytoplasmic proton extrusion coupled with ATP generation
Authors: Gao, Jun and Zhang, Tian and Kang, Zhanfang and Ting, Weijen and Xu, Lingqing and Yin, Dazhong
Journal: Molecular Immunology (2017): 219--226
Authors: Gao, Jun and Zhang, Tian and Kang, Zhanfang and Ting, Weijen and Xu, Lingqing and Yin, Dazhong
Journal: Molecular Immunology (2017): 219--226
Oxidative Stress-Activated NHE1 Is Involved in High Glucose-Induced Apoptosis in Renal Tubular Epithelial Cells
Authors: Wu, Yiqing and Zhang, Min and Liu, Rui and Zhao, Chunjie
Journal: Yonsei Medical Journal (2016): 1252--1259
Authors: Wu, Yiqing and Zhang, Min and Liu, Rui and Zhao, Chunjie
Journal: Yonsei Medical Journal (2016): 1252--1259
References
View all 34 references: Citation Explorer
Simultaneous measurement of water volume and pH in single cells using BCECF and fluorescence imaging microscopy
Authors: Alvarez-Leefmans FJ, Herrera-Perez JJ, Marquez MS, Blanco VM.
Journal: Biophys J (2006): 608
Authors: Alvarez-Leefmans FJ, Herrera-Perez JJ, Marquez MS, Blanco VM.
Journal: Biophys J (2006): 608
Photophysics of the fluorescent pH indicator BCECF
Authors: Boens N, Qin W, Basaric N, Orte A, Talavera EM, Alvarez-Pez JM.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 9334
Authors: Boens N, Qin W, Basaric N, Orte A, Talavera EM, Alvarez-Pez JM.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 9334
Drug efflux transport properties of 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (bcecf-am) and its fluorescent free acid, bcecf
Authors: Bachmeier CJ, Trickler WJ, Miller DW.
Journal: J Pharm Sci (2004): 932
Authors: Bachmeier CJ, Trickler WJ, Miller DW.
Journal: J Pharm Sci (2004): 932
A rapid method for measuring intracellular pH using BCECF-AM
Authors: Ozkan P, Mutharasan R.
Journal: Biochim Biophys Acta (2002): 143
Authors: Ozkan P, Mutharasan R.
Journal: Biochim Biophys Acta (2002): 143
Detection of MRP functional activity: calcein AM but not BCECF AM as a Multidrug Resistance-related Protein (MRP1) substrate
Authors: Olson DP, Taylor BJ, Ivy SP.
Journal: Cytometry (2001): 105
Authors: Olson DP, Taylor BJ, Ivy SP.
Journal: Cytometry (2001): 105
In vivo application of intestinal ph measurement using 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (bcecf) fluorescence imaging
Authors: Marechal X, Mordon S, Devoisselle JM, Begu S, Guery B, Neviere R, Buys B, Dhelin G, Lesage JC, Mathieu D, Chopin C.
Journal: Photochem Photobiol (1999): 813
Authors: Marechal X, Mordon S, Devoisselle JM, Begu S, Guery B, Neviere R, Buys B, Dhelin G, Lesage JC, Mathieu D, Chopin C.
Journal: Photochem Photobiol (1999): 813
Fluorescence probe (BCECF) loading efficiency in human platelets depends on cell concentration: application to pHi measurements
Authors: Ruiz-Palomo F, Garcia C, Gomez M, Revenga M.
Journal: Clin Biochem (1999): 391
Authors: Ruiz-Palomo F, Garcia C, Gomez M, Revenga M.
Journal: Clin Biochem (1999): 391
Analysis of the uptake of the fluorescent marker 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (bcecf) by hydrogenosomes in trichomonas vaginalis
Authors: Scott DA, Docampo R, Benchimol M.
Journal: Eur J Cell Biol (1998): 139
Authors: Scott DA, Docampo R, Benchimol M.
Journal: Eur J Cell Biol (1998): 139
Simultaneous detection of cell volume and intracellular pH in isolated rat duodenal cells by confocal microscopy and BCECF
Authors: Weinlich M, Heydasch U, Mooren F, Starlinger M.
Journal: Res Exp Med (Berl) (1998): 73
Authors: Weinlich M, Heydasch U, Mooren F, Starlinger M.
Journal: Res Exp Med (Berl) (1998): 73
BCECF in single cultured cells: inhomogeneous distribution but homogeneous response
Authors: Weinlich M, Theiss C, Lin CT, Kinne RK.
Journal: J Exp Biol (1998): 57
Authors: Weinlich M, Theiss C, Lin CT, Kinne RK.
Journal: J Exp Biol (1998): 57
Application notes
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
FAQ
How to measure intracellular pH using flow cytometry?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What are the advantages of a Nanodrop light spectrophotometer?
What are the disadvantages of Nanodrop light spectrophotometer?
What is an AM ester?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What are the advantages of a Nanodrop light spectrophotometer?
What are the disadvantages of Nanodrop light spectrophotometer?
What is an AM ester?