RatioWorks™ PDMPO

The existing pH probes are ill-adapted to study acidic organelles such as lysosomes, endosomes, spermatozoa and acrosomes because their fluorescence is significantly reduced at lower pH. In addition, most of the existing pH probes (such as BCECF and SNARF) are not selectively localized in acidic organelles. The growing potential of ratio imaging is significantly limited by the lack of appropriate fluorescent probes for acidic organelles although ratio imaging has received intensive attention in the past few decades. PDMPO [2-(4-pyridyl)-5-((4-(2-dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole] is the same molecule to LysoSensor Yellow/Blue, and characterized as acidotropic dual-excitation and dual-emission pH probe. It emits intense yellow fluorescence at lower pH and gives intense blue fluorescence at higher pH. This unique pH-dependent fluorescence makes PDMPO an ideal pH probe for acidic organelles with pKa = 4.47. PDMPO selectively labels acidic organelles (such as lysosomes) of live cells and the two distinct emission peaks can be used to monitor the pH fluctuations of live cells in ratio measurements. Additionally, the very large Stokes shift and excellent photostability of PDMPO make PDMPO an excellent fluorescent acidotropic reagent for fluorescence imaging. The unique fluorescence properties of PDMPO might give researchers a new tool with which to study acidic organelles of live cells. PDMPO can be well excited by the violet laser at 405 nm for flow cytometric applications.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21204	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of RatioWorks™ PDMPO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	272.918 µL	1.365 mL	2.729 mL	13.646 mL	27.292 mL
5 mM	54.584 µL	272.918 µL	545.837 µL	2.729 mL	5.458 mL
10 mM	27.292 µL	136.459 µL	272.918 µL	1.365 mL	2.729 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	366.41 g/mol	=	mL	x	mM	=	mmol

Citations

View all 7 citations: Citation Explorer

Sequestosome-1 (SQSTM1/p62) as a target in dopamine catabolite-mediated cellular dyshomeostasis

Authors:

Masato, Anna and Andolfo, Annapaola and Favetta, Giulia and Bellini, Edoardo Niccol{\`o} and Cogo, Susanna and Dalla Valle, Luisa and Boassa, Daniela and Greggio, Elisa and Plotegher, Nicoletta and Bubacco, Luigi

Journal:

Cell Death \& Disease (2024): 424

Mycobacterium tuberculosis Utilizes Host Histamine Receptor H1 to Modulate Reactive Oxygen Species Production and Phagosome Maturation via the p38MAPK-NOX2 Axis

Authors:

Mo, Siwei and Guo, Jiubiao and Ye, Taosheng and Zhang, Ximeng and Zeng, Jiang and Xu, Yuzhong and Peng, Bin and Dai, Youchao and Xiao, Wei and Zhang, Peize and others,

Journal:

Mbio (2022): e02004--22

Mannose ameliorates experimental colitis by protecting intestinal barrier integrity

Authors:

Dong, Lijun and Xie, Jingwen and Wang, Youyi and Jiang, Honglian and Chen, Kai and Li, Dantong and Wang, Jing and Liu, Yunzhi and He, Jia and Zhou, Jia and others,

Journal:

Nature communications (2022): 1--17

The molecular basis for pore pattern morphogenesis in diatom silica

Authors:

Heintze, Christoph and Babenko, Iaroslav and Suchanova, Jirina Zackova and Skeffington, Alastair and Friedrich, Benjamin and Kroeger, Nils

An intimate view into the silica deposition vesicles of diatoms

Authors:

Heintze, Christoph and Formanek, Petr and Pohl, Darius and Hauptstein, Jannes and Rellinghaus, Bernd and Kr{\"o}ger, Nils

Journal:

BMC Materials (2020): 1--15

References

View all 8 references: Citation Explorer

Using LysoSensor Yellow/Blue DND-160 to sense acidic pH under high hydrostatic pressures

Authors:

DePedro HM, Urayama P.

Journal:

Anal Biochem (2009): 359

Heterogeneity of acid secretion induced by carbachol and histamine along the gastric gland axis and its relationship to [Ca2+]i

Authors:

Perez-Zoghbi JF, Mayora A, Ruiz MC, Michelangeli F.

Journal:

Am J Physiol Gastrointest Liver Physiol (2008): G671

Acidic microclimate pH distribution in PLGA microspheres monitored by confocal laser scanning microscopy

Authors:

Ding AG, Schwendeman SP.

Journal:

Pharm Res (2008): 2041

Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter

Authors:

Schroder HC, Perovic-Ottstadt S, Rothenberger M, Wiens M, Schwertner H, Batel R, Korzhev M, Muller IM, Muller WE.

Journal:

Biochem J (2004): 665

Divalent cations regulate acidity within the lumen and tubulovesicle compartment of gastric parietal cells

Authors:

Gerbino A, Hofer AM, McKay B, Lau BW, Soybel DI.

Journal:

Gastroenterology (2004): 182

Physical properties

Molecular weight	366.41
Solvent	DMSO

Spectral properties

Excitation (nm)	333
Emission (nm)	531

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 11, 2025