ReadiUse™ Disposable PD-10 Desalting Column

ReadiUse™ Disposable PD-10 Desalting Column is a prepacked gel filtration column for rapid desalting, buffer exchange, and removal of small-molecule contaminants from biological samples.

Sephadex G-25 Resin: Enables size-exclusion separation of macromolecules from small contaminants.
High Salt Removal: Removes over 98% of salts by gravity flow or over 90% by centrifugation.
High Sample Recovery: Typically achieves 70% to over 95% recovery.
Flexible Workflow: Supports gravity-flow and centrifugation protocols.
Applications: Suitable for buffer exchange, desalting, and removal of unreacted dyes.

ReadiUse™ Disposable PD-10 Desalting Column packed with Sephadex G-25 resin.

Protocol

COA

Catalog	Size	Price	Quantity
60504	5 Columns	Price
60505	10 Columns	Price

Overview

ReadiUse™ Disposable PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (e.g., salts, dyes, radioactive labels) from samples using gravity flow or centrifugation. It is an inexpensive and convenient alternative to lengthy and tedious dialysis procedures. The desalting and buffer exchange is run by gravity flow (1.0 to 2.5 mL samples) or centrifugation (1.75 to 2.5 mL samples). The salt removal rate is typically >98% salt with gravity and >90% with centrifugation. The sample recovery is typically in the range of 70% to >95%.

References

View all 5 references: Citation Explorer

Fully Automated 89Zr Labeling and Purification of Antibodies.

Authors:

Poot, Alex J and Adamzek, Kevin W A and Windhorst, Albert D and Vosjan, Maria J W D and Kropf, Saskia and Wester, Hans-Jurgen and van Dongen, Guus A M S and Vugts, Danielle J

Journal:

Journal of nuclear medicine : official publication, Society of Nuclear Medicine (2019): 691-695

Recombinant production of A1S_0222 from Acinetobacter baumannii ATCC 17978 and confirmation of its DNA-(adenine N6)-methyltransferase activity.

Authors:

Blaschke, Ulrike and Suwono, Beneditta and Zafari, Sachli and Ebersberger, Ingo and Skiebe, Evelyn and Jeffries, Cy M and Svergun, Dmitri I and Wilharm, Gottfried

Journal:

Protein expression and purification (2018): 78-85

Synthesis and characterization of (18)F-labeled active site inhibited factor VII (ASIS).

Authors:

Erlandsson, Maria and Nielsen, Carsten H and Jeppesen, Troels E and Kristensen, Jesper B and Petersen, Lars C and Madsen, Jacob and Kjaer, Andreas

Journal:

Journal of labelled compounds & radiopharmaceuticals (2015): 196-201

In vitro and in vivo evaluation of direct rhenium-188-labeled anti-CD52 monoclonal antibody alemtuzumab for radioimmunotherapy of B-cell chronic lymphocytic leukemia.

Authors:

De Decker, Mario and Bacher, Klaus and Thierens, Hubert and Slegers, Guido and Dierckx, Rudi A and De Vos, Filip

Journal:

Nuclear medicine and biology (2008): 599-604

Different techniques for urinary protein analysis of normal and lung cancer patients.

Authors:

Tantipaiboonwong, Payungsak and Sinchaikul, Supachok and Sriyam, Supawadee and Phutrakul, Suree and Chen, Shui-Tein

Journal:

Proteomics (2005): 1140-9

Physical properties

Molecular weight	N/A
Solvent	Water

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C)

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Page updated on October 18, 2026