AAT Bioquest

ReadiUse™ mammalian cell lysis buffer *5X*

ReadiUse™ Mammalian Cell Lysis Buffer can be used for solution-based biochemical assays.
ReadiUse™ Mammalian Cell Lysis Buffer can be used for solution-based biochemical assays.
ReadiUse™ Mammalian Cell Lysis Buffer can be used for solution-based biochemical assays.
Time course of TRTS-induced AChE activity in PC12 cells. To examine AChE activity (a biochemical marker for neuronal differentiation) in PC12 cells, the choline produced from acetylcholine by endogenous AChE in each sample was quantified using the Amplite Fluorimetric Acetylcholine Esterase Assay Kit (AAT Bioquest, Sunnyvale, CA, USA) with ReadiUse mammalian cell lysis buffer *5X* (AAT Bioquest) in accordance with the manufacturer’s instructions. Cell lysates from PC12 cells in the differentiation medium were prepared and analyzed for acetylcholine esterase (AChE) activity after treatment with TRTS at 39.5°C (18 h/day) or 40 ng/ml BMP4 for the indicated number of days. The data represent the mean ± SD of three replicates. **P < 0.01 vs. the day 0 control with no stimulation. n.s., not significant vs. the day 0 control with no treatment. BMP4, bone morphogenetic protein 4, TRTS, temperature-controlled repeated thermal stimulation. Source: <b>Induction of Neurite Outgrowth in PC12 Cells Treated with Temperature-Controlled Repeated Thermal Stimulation</b> by Kudo, Tada-aki et.al., <em>PloS one</em>, April 2015
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Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C)
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All our ReadiUse™ reagents require minimal hands-on time. This mammalian cell lysis buffer just require a simple 5-fold dilution.



View all 8 citations: Citation Explorer
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Journal: Molecular Medicine Reports (2016): 3595--3601
Induction of Neurite Outgrowth in PC12 Cells Treated with Temperature-Controlled Repeated Thermal Stimulation
Authors: Kudo, Tada-aki and Kanetaka, Hiroyasu and Mochizuki, Kentaro and Tominami, Kanako and Nunome, Shoko and Abe, Genji and Kosukegawa, Hiroyuki and Abe, Toshihiko and Mori, Hitoshi and Mori, Kazumi and others, undefined
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