ReadiUse™ mammalian cell lysis buffer 5X

ReadiUse™ Mammalian Cell Lysis Buffer can be used for solution-based biochemical assays.

Time course of TRTS-induced AChE activity in PC12 cells. To examine AChE activity (a biochemical marker for neuronal differentiation) in PC12 cells, the choline produced from acetylcholine by endogenous AChE in each sample was quantified using the Amplite Fluorimetric Acetylcholine Esterase Assay Kit (AAT Bioquest, Sunnyvale, CA, USA) with ReadiUse mammalian cell lysis buffer *5X* (AAT Bioquest) in accordance with the manufacturer’s instructions. Cell lysates from PC12 cells in the differentiation medium were prepared and analyzed for acetylcholine esterase (AChE) activity after treatment with TRTS at 39.5°C (18 h/day) or 40 ng/ml BMP4 for the indicated number of days. The data represent the mean ± SD of three replicates. **P < 0.01 vs. the day 0 control with no stimulation. n.s., not significant vs. the day 0 control with no treatment. BMP4, bone morphogenetic protein 4, TRTS, temperature-controlled repeated thermal stimulation. Source: <b>Induction of Neurite Outgrowth in PC12 Cells Treated with Temperature-Controlled Repeated Thermal Stimulation</b> by Kudo, Tada-aki et.al., <em>PloS one</em>, April 2015

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C)
UNSPSC	12352200

Overview SDS Protocol

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Figure 1. ReadiUse™ Mammalian Cell Lysis Buffer can be used for solution-based biochemical assays.

Figure 2. Time course of TRTS-induced AChE activity in PC12 cells. To examine AChE activity (a biochemical marker for neuronal differentiation) in PC12 cells, the choline produced from acetylcholine by endogenous AChE in each sample was quantified using the Amplite Fluorimetric Acetylcholine Esterase Assay Kit (AAT Bioquest, Sunnyvale, CA, USA) with ReadiUse mammalian cell lysis buffer *5X* (AAT Bioquest) in accordance with the manufacturer’s instructions. Cell lysates from PC12 cells in the differentiation medium were prepared and analyzed for acetylcholine esterase (AChE) activity after treatment with TRTS at 39.5°C (18 h/day) or 40 ng/ml BMP4 for the indicated number of days. The data represent the mean ± SD of three replicates. **P < 0.01 vs. the day 0 control with no stimulation. n.s., not significant vs. the day 0 control with no treatment. BMP4, bone morphogenetic protein 4, TRTS, temperature-controlled repeated thermal stimulation. Source: Induction of Neurite Outgrowth in PC12 Cells Treated with Temperature-Controlled Repeated Thermal Stimulation by Kudo, Tada-aki et.al., PloS one, April 2015

Citations

View all 7 citations: Citation Explorer

Gene discovery in inherited retinal diseases using whole genome sequencing and autozygome based analysis
Authors: Bedoni, Nicola
Journal: (2019)

Rhinacanthin C Alleviates Amyloid-β Fibrils' Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-β, and Interferon-γ in Glial Cells
Authors: Chuang, Kai-An and Li, Ming-Han and Lin, Ni-Hsuan and Chang, Chih-Hsuan and Lu, I and Pan, I and Takahashi, Tomoya and Perng, Ming-Der and Wen, Shu-Fang and others, undefined
Journal: Oxidative Medicine and Cellular Longevity (2017)

Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage
Authors: Zhang, Xiaoying and Zhu, Cuicui and Luo, Qiong and Dong, Jv and Liu, Lv and Li, Min and Zhu, Hongtao and Ma, Xiangping and Wang, Jun
Journal: Molecular Medicine Reports (2016): 3595--3601

Induction of Neurite Outgrowth in PC12 Cells Treated with Temperature-Controlled Repeated Thermal Stimulation
Authors: Kudo, Tada-aki and Kanetaka, Hiroyasu and Mochizuki, Kentaro and Tominami, Kanako and Nunome, Shoko and Abe, Genji and Kosukegawa, Hiroyuki and Abe, Toshihiko and Mori, Hitoshi and Mori, Kazumi and others, undefined
Journal: PloS one (2015): e0124024

Analysis of potassium iodate reduction in tissue homogenates using high performance liquid chromatography--inductively coupled plasma-mass spectrometry
Authors: Cao, Xiaoxiao and Ma, Wei and Liu, Liejun and Xu, Jing and Wang, Haiyan and Li, Xiuwei and Wang, Jiangqing and Zhang, Jianhua and Wang, Zexi and Gu, Yunyou
Journal: Journal of Trace Elements in Medicine and Biology (2015): 1--6

Application of interleukin-22 mediates protection in experimental acetaminophen-induced acute liver injury
Authors: Scheiermann, Patrick and Bachmann, Malte and Goren, Itamar and Zwissler, Bernhard and Pfeilschifter, Josef and Mühl, Heiko
Journal: The American journal of pathology (2013): 1107--1113

Synergistic activity of αvβ3 integrins and the elastin binding protein enhance cell-matrix interactions on bioactive hydrogel surfaces
Authors: Patel, Dhaval and V, undefined and romme, Susan E and Reid, Michael E and Taite, Lakeshia J
Journal: Biomacromolecules (2012): 1420--1428

References

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