ReadiUse™ Preactivated PerCP-Cy5.5 Maleimide

ReadiUse™ Preactivated PerCP-Cy5.5 Tandem is a preactivated tandem fluorophore reagent for conjugating PerCP-Cy5.5 to antibodies and other proteins for multicolor flow cytometry.

Fluorescence Detection: Excitation at 489 nm and emission at 679 nm.
Maleimide Chemistry: SMCC-activated for thiol-reactive conjugation.
Ready-to-Use: Preactivated dye simplifies conjugation workflows.
Instrument Compatibility: Excitable with a standard 488 nm laser.
Applications: Supports multicolor flow cytometry alongside FITC, PE, and other fluorochromes.

Datasheet

SDS

Catalog	Size	Price	Quantity
2724	1 mg	Price

Overview

PerCP-Cy5.5 is a popular tandem color used in flow cytometry. Its primary absorption peak is at ~480 nm with emission peak at ~700 nm. AAT Bioquest offers this preactivated PerCP-Cy5.5 to facilitate the PerCP-Cy5.5 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated PerCP-Cy5.5 maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. Its tandem conjugates (such as PerCP-Cy5.5) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers.

References

View all 10 references: Citation Explorer

Spurious CD34 expression in B-cell lymphoma due to nonspecific binding to PerCP-Cy5.5 fluorochrome conjugates: A rare phenomenon and a diagnostic pitfall.

Authors:

Shi, Min and Timm, Michael M and Howard, Matthew T and Jevremovic, Dragan and Yuan, Ji and Greipp, Patricia T and Peterson, Jess F and Roh, Dana J and Horna, Pedro and Olteanu, Horatiu

Journal:

Cytometry. Part B, Clinical cytometry (2022)

[Clinical features of imbalance between Th1 and Th22 cells and its association with disease progression in patients with liver cirrhosis].

Authors:

Wu, H Q and Zhao, J J and Li, H W and Zhang, Z

Journal:

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (2017): 738-744

Inhibition of histone deacetylase 6 restores innate immune cells in the bone marrow in a lethal septic model.

Authors:

Zhao, Ting and Li, Yongqing and Liu, Baoling and Pan, Baihong and Cheng, Xin and Georgoff, Patrick and Alam, Hasan B

Journal:

The journal of trauma and acute care surgery (2016): 34-40; discussion 40-1

Flow cytometric analysis of T lymphocyte proliferation in vivo by EdU incorporation.

Authors:

Sun, Xiaojing and Zhang, Chunpan and Jin, Hua and Sun, Guangyong and Tian, Yue and Shi, Wen and Zhang, Dong

Journal:

International immunopharmacology (2016): 56-65

Combined normal donor and CLL: Single tube ZAP-70 analysis.

Authors:

Degheidy, Heba A and Venzon, David J and Farooqui, Mohammed Z H and Abbasi, Fatima and Arthur, Diane C and Wilson, Wyndham H and Wiestner, Adrian and Stetler-Stevenson, M A and Marti, Gerald E

Journal:

Cytometry. Part B, Clinical cytometry (2012): 67-77

Physical properties

Molecular weight	N/A
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	350000
Excitation (nm)	482
Emission (nm)	695

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Page updated on October 15, 2026