ReadiUse™ Preactivated PerCP-Cy5.5 Maleimide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	N/A
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	350000
Excitation (nm)	482
Emission (nm)	695

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

N/A

Extinction coefficient (cm ^-1 M ^-1)

350000

Excitation (nm)

482

Emission (nm)

695

PerCP-Cy5.5 is a popular tandem color used in flow cytometry. Its primary absorption peak is at ~480 nm with emission peak at ~700 nm. AAT Bioquest offers this preactivated PerCP-Cy5.5 to facilitate the PerCP-Cy5.5 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated PerCP-Cy5.5 maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. Its tandem conjugates (such as PerCP-Cy5.5) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	350000
Excitation (nm)	482
Emission (nm)	695

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
ReadiUse™ Preactivated PerCP-Cy5.5 Tandem	482	695	350000
ReadiUse™ Preactivated PE-Cy5.5 Maleimide	565	671	1960000
ReadiUse™ Preactivated APC-Cy5.5 Maleimide	651	700	700000

References

View all 10 references: Citation Explorer

Spurious CD34 expression in B-cell lymphoma due to nonspecific binding to PerCP-Cy5.5 fluorochrome conjugates: A rare phenomenon and a diagnostic pitfall.
Authors: Shi, Min and Timm, Michael M and Howard, Matthew T and Jevremovic, Dragan and Yuan, Ji and Greipp, Patricia T and Peterson, Jess F and Roh, Dana J and Horna, Pedro and Olteanu, Horatiu
Journal: Cytometry. Part B, Clinical cytometry (2022)

[Clinical features of imbalance between Th1 and Th22 cells and its association with disease progression in patients with liver cirrhosis].
Authors: Wu, H Q and Zhao, J J and Li, H W and Zhang, Z
Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (2017): 738-744

Flow cytometric analysis of T lymphocyte proliferation in vivo by EdU incorporation.
Authors: Sun, Xiaojing and Zhang, Chunpan and Jin, Hua and Sun, Guangyong and Tian, Yue and Shi, Wen and Zhang, Dong
Journal: International immunopharmacology (2016): 56-65

Inhibition of histone deacetylase 6 restores innate immune cells in the bone marrow in a lethal septic model.
Authors: Zhao, Ting and Li, Yongqing and Liu, Baoling and Pan, Baihong and Cheng, Xin and Georgoff, Patrick and Alam, Hasan B
Journal: The journal of trauma and acute care surgery (2016): 34-40; discussion 40-1

PE-Cy5.5 conjugates bind to the cells expressing mouse DEC205/CD205.
Authors: Park, Chae Gyu and Rodriguez, Anthony and Steinman, Ralph M
Journal: Journal of immunological methods (2012): 184-90

Combined normal donor and CLL: Single tube ZAP-70 analysis.
Authors: Degheidy, Heba A and Venzon, David J and Farooqui, Mohammed Z H and Abbasi, Fatima and Arthur, Diane C and Wilson, Wyndham H and Wiestner, Adrian and Stetler-Stevenson, M A and Marti, Gerald E
Journal: Cytometry. Part B, Clinical cytometry (2012): 67-77

[The relationship between dysfunction of cell-mediated immunity and prognosis in severely burned patients].
Authors: Dong, Ning and Jin, Bo-quan and Yao, Yong-ming and Yu, Yan and Sheng, Zhi-yong
Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (2008): 516-9

[Clinical significance of changes in quantitative expression of human leukocyte antigen DR in severely burned patients].
Authors: Dong, Ning and Yao, Yong-ming and Cao, Yu-jue and He, Li-xin and Chai, Jia-ke and Xu, Shan and Sheng, Zhi-yong
Journal: Zhonghua wai ke za zhi [Chinese journal of surgery] (2007): 766-9

Rare event counting of CD59- red cells in human blood: A 47-month experience using PNH consensus guidelines for WBC and RBC testing in a reference lab.
Authors: Liew, Michael and Farley, Marjorie and Andreasen, John and Parker, Charles J and Wittwer, Carl T
Journal: Cytometry. Part B, Clinical cytometry : 261-9

Analysis of bone marrow plasma cells in patients with solitary bone plasmacytoma.
Authors: Bhaskar, Archana and Gupta, Ritu and Sharma, Atul and Kumar, Lalit and Jain, Paresh
Journal: Cancer therapy : 49-52