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ReadiUse™ Preactivated PerCP-Cy5.5 Tandem
PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). Its tandem conjugates (such as PerCP-Cy5.5) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers. AAT Bioquest offers this preactivated PerCP-Cy5.5 Tandem to facilitate the PerCP-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PerCP-Cy5.5 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PerCP-Cy5.5 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
Conjugation scheme for ReadiUse™ Preactivated PerCP-Cy5.5 Tandem. The activated PerCP-Cy5.5 tandem is premodified with our Buccutite™ FOL and can be readily used for conjugation. To conjugate your desired antibody or protein, first modify it with our Buccutite™ MTA (provided) and then mix both components. The Buccutite™ MTA-modified protein will readily react with the Buccutite™ FOL-activated PerCP-Cy5.5 tandem to give the desired PerCP-Cy5.5-antibody conjugate in much higher yield than SMCC chemistry. In addition our preactivated PerCP-Cy5.5 tandem reacts with MTA-modified biopolymers at a much lower concentration than SMCC chemistry.
Conjugation scheme for ReadiUse™ Preactivated PerCP-Cy5.5 Tandem. The activated PerCP-Cy5.5 tandem is premodified with our Buccutite™ FOL and can be readily used for conjugation. To conjugate your desired antibody or protein, first modify it with our Buccutite™ MTA (provided) and then mix both components. The Buccutite™ MTA-modified protein will readily react with the Buccutite™ FOL-activated PerCP-Cy5.5 tandem to give the desired PerCP-Cy5.5-antibody conjugate in much higher yield than SMCC chemistry. In addition our preactivated PerCP-Cy5.5 tandem reacts with MTA-modified biopolymers at a much lower concentration than SMCC chemistry.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
25951 mg
Price
 
Physical properties
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)
350000
Excitation (nm)482
Emission (nm)695
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025