Name: ReadiUse™ Preactivated PerCP-Cy5.5 Tandem
Brand: AAT Bioquest
SKU: 2595
Price: 415 USD
Availability: InStock

ReadiUse™ Preactivated PerCP-Cy5.5 Tandem

ReadiUse™ Preactivated PerCP-Cy5.5 Tandem is a preactivated tandem fluorophore reagent for conjugating PerCP-Cy5.5 to antibodies and other proteins for multicolor flow cytometry.

Fluorescence Detection: Excitation at 482 nm and emission at 695 nm.
Conjugation Chemistry: Buccutite™ FOL/MTA chemistry enables amine-based protein conjugation.
High Conjugation Yield: Higher yields than conventional SMCC-based chemistry.
Instrument Compatibility: Compatible with standard 488 nm laser excitation.
Applications: Supports multicolor flow cytometry alongside FITC, PE, and other fluorochromes.

Conjugation scheme for ReadiUse™ Preactivated PerCP-Cy5.5 Tandem. The activated PerCP-Cy5.5 tandem is premodified with our Buccutite™ FOL and can be readily used for conjugation. To conjugate your desired antibody or protein, first modify it with our Buccutite™ MTA (provided) and then mix both components. The Buccutite™ MTA-modified protein will readily react with the Buccutite™ FOL-activated PerCP-Cy5.5 tandem to give the desired PerCP-Cy5.5-antibody conjugate in much higher yield than SMCC chemistry. In addition our preactivated PerCP-Cy5.5 tandem reacts with MTA-modified biopolymers at a much lower concentration than SMCC chemistry.

Protocol

SDS

Catalog	Size	Price	Quantity
2595	1 mg	Price

Overview

PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). Its tandem conjugates (such as PerCP-Cy5.5) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers. AAT Bioquest offers this preactivated PerCP-Cy5.5 Tandem to facilitate the PerCP-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PerCP-Cy5.5 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PerCP-Cy5.5 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	350000
Excitation (nm)	482
Emission (nm)	695

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 15, 2026

Component A: ReadiUse™ Preactivated PerCP-Cy5.5 Tandem	1 mg
Component B: Buccutite™ MTA	1 vial (100 ug)
Component C: Spin Desalting Column	Not Included