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ReadiUse™ Preactivated Streptavidin Alkyne

ReadiUse™ Preactivated Streptavidin Alkyne is an excellent azido-reactive building block that can be used for developing bioconjugates via the well-known click chemistry. It reacts with conventional azide compounds. It more readily reacts with the FastClick™ azido compounds than the conventional azide compounds. Streptavidin binds 4 moles of biotin per mole of protein with a high affinity virtually unmatched in nature. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality where most useful biological interactions occur (pI of 5-6 vs 10 for avidin). As a result, streptavidin frequently exhibits lower levels of non-specific binding than does avidin when the proteins are used in applications relying upon the formation of avidin/biotin complexes. Streptavidin finds utility in a variety of biological systems because antibody molecules are easily modified by the covalent attachment of derivatives of biotin with little or no loss in the ability of the antibody molecules to bind their antigens. These biotinylated antibodies may be detected by their interaction with conjugates of streptavidin and the reporter enzymes. The same preparation of conjugated streptavidin reporter enzyme may be used with any number of different biotinylated antibodies making this system a highly flexible one. The reporter molecule may be bound to streptavidin covalently, or biotinylated and attached to streptavidin via the streptavidin-biotin interaction.

References

View all 24 references: Citation Explorer
Enhanced Stable Cavitation and Nonlinear Acoustic Properties of Poly(butyl cyanoacrylate) Polymeric Microbubbles after Bioconjugation.
Authors: Barmin, Roman A and Dasgupta, Anshuman and Rix, Anne and Weiler, Marek and Appold, Lia and Rütten, Stephan and Padilla, Frederic and Kuehne, Alexander J C and Pich, Andrij and De Laporte, Laura and Kiessling, Fabian and Pallares, Roger M and Lammers, Twan
Journal: ACS biomaterials science & engineering (2024): 75-81
Gold-Nanoparticle-Coated Magnetic Beads for ALP-Enzyme-Based Electrochemical Immunosensing in Human Plasma.
Authors: Lee, Seo-Eun and Jeong, Se-Eun and Hong, Jae-Sang and Im, Hyungsoon and Hwang, Sei-Young and Oh, Jun Kyun and Kim, Seong-Eun
Journal: Materials (Basel, Switzerland) (2022)
Multiple amplification-based fluorometric aptasensor for highly sensitive detection of Staphylococcus aureus.
Authors: Chen, Wei and Zhang, Yanke and Lai, Qingteng and Li, Youzhen and Liu, Zhengchun
Journal: Applied microbiology and biotechnology (2022): 6733-6743
Antibody- and aptamer-based competitive fluorescence polarization/anisotropy assays for ochratoxin A with tetramethylrhodamine-labeled ochratoxin A.
Authors: Li, Yapiao and Zhao, Qiang
Journal: Analytical methods : advancing methods and applications (2021): 1612-1617
Immobilization of recombinant lysostaphin on nanoparticle through biotin-streptavidin conjugation technology as a geometrical progressed confrontation against Staphylococcus aureus infection.
Authors: Ehsani, Gelareh and Farahnak, Maryam and Norouzian, Dariush and Ehsani, Parastoo
Journal: Biotechnology and applied biochemistry (2021): 1058-1066
Page updated on April 25, 2025

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Catalog Number16884
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Physical properties

Solvent

Water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure