Name: ReadiUse™ Preactivated Streptavidin Alkyne
Brand: AAT Bioquest
SKU: 16884
Price: 412 USD
Availability: InStock

ReadiUse™ Preactivated Streptavidin Alkyne

ReadiUse™ Preactivated Streptavidin Alkyne is an azide-reactive streptavidin reagent for developing bioconjugates through click chemistry.

Click Chemistry: Alkyne groups react with azide-containing compounds.
Enhanced Reactivity: Reacts more readily with FastClick™ azides.
High Biotin Affinity: Binds up to 4 moles of biotin per streptavidin.
Low Background: Reduced nonspecific binding compared to avidin.
Applications: Suitable for bioconjugation and biotin-based detection assays.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
16884	1 mg	Price

Overview

ReadiUse™ Preactivated Streptavidin Alkyne is an excellent azido-reactive building block that can be used for developing bioconjugates via the well-known click chemistry. It reacts with conventional azide compounds. It more readily reacts with the FastClick™ azido compounds than the conventional azide compounds. Streptavidin binds 4 moles of biotin per mole of protein with a high affinity virtually unmatched in nature. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality where most useful biological interactions occur (pI of 5-6 vs 10 for avidin). As a result, streptavidin frequently exhibits lower levels of non-specific binding than does avidin when the proteins are used in applications relying upon the formation of avidin/biotin complexes. Streptavidin finds utility in a variety of biological systems because antibody molecules are easily modified by the covalent attachment of derivatives of biotin with little or no loss in the ability of the antibody molecules to bind their antigens. These biotinylated antibodies may be detected by their interaction with conjugates of streptavidin and the reporter enzymes. The same preparation of conjugated streptavidin reporter enzyme may be used with any number of different biotinylated antibodies making this system a highly flexible one. The reporter molecule may be bound to streptavidin covalently, or biotinylated and attached to streptavidin via the streptavidin-biotin interaction.

References

View all 24 references: Citation Explorer

Enhanced Stable Cavitation and Nonlinear Acoustic Properties of Poly(butyl cyanoacrylate) Polymeric Microbubbles after Bioconjugation.

Authors:

Barmin, Roman A and Dasgupta, Anshuman and Rix, Anne and Weiler, Marek and Appold, Lia and Rütten, Stephan and Padilla, Frederic and Kuehne, Alexander J C and Pich, Andrij and De Laporte, Laura and Kiessling, Fabian and Pallares, Roger M and Lammers, Twan

Journal:

ACS biomaterials science & engineering (2024): 75-81

Gold-Nanoparticle-Coated Magnetic Beads for ALP-Enzyme-Based Electrochemical Immunosensing in Human Plasma.

Authors:

Lee, Seo-Eun and Jeong, Se-Eun and Hong, Jae-Sang and Im, Hyungsoon and Hwang, Sei-Young and Oh, Jun Kyun and Kim, Seong-Eun

Journal:

Materials (Basel, Switzerland) (2022)

Multiple amplification-based fluorometric aptasensor for highly sensitive detection of Staphylococcus aureus.

Authors:

Chen, Wei and Zhang, Yanke and Lai, Qingteng and Li, Youzhen and Liu, Zhengchun

Journal:

Applied microbiology and biotechnology (2022): 6733-6743

Antibody- and aptamer-based competitive fluorescence polarization/anisotropy assays for ochratoxin A with tetramethylrhodamine-labeled ochratoxin A.

Authors:

Li, Yapiao and Zhao, Qiang

Journal:

Analytical methods : advancing methods and applications (2021): 1612-1617

Immobilization of recombinant lysostaphin on nanoparticle through biotin-streptavidin conjugation technology as a geometrical progressed confrontation against Staphylococcus aureus infection.

Authors:

Ehsani, Gelareh and Farahnak, Maryam and Norouzian, Dariush and Ehsani, Parastoo

Journal:

Biotechnology and applied biochemistry (2021): 1058-1066

Physical properties

Solvent

Water

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 25, 2026