ReadiUse™ Yeast Cell Lysis Buffer *5X*

ReadiUse™ Yeast Cell Lysis Buffer

ReadiUse™ Yeast Cell Lysis Buffer *5X* is a detergent-based reagent for lysing yeast cells and solubilizing cellular components in laboratory applications.

Lysis Method: Uses a mild detergent-based procedure to disrupt yeast cell membranes.
Protein Preservation: Releases solubilized, functionally active proteins without harsh mechanical disruption.
Workflow: Requires only a simple 5-fold dilution before use.
Versatility: Also supports lysis of Bacillus subtilis and Gram-positive and Gram-negative bacteria.
Applications: Suitable for protein extraction, purification, molecular biology, and biomolecular analysis.

Yeast cell lysis refers to the breaking down of yeast cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules, and other small biomolecules. Depending upon the detergents used, either all or some membranes are lysed. ReadiUse™ reagents require minimal hands-on time. This yeast cell lysis buffer just requires a simple 5-fold dilution.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
24102	10 mL	Price

Overview

ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilization in laboratory applications. Engineered to simplify the extraction of cell components, including proteins, lipids, nucleic acids, reporter molecules, and small biomolecules, this buffer employs a detergent-based lysis procedure for the gentle breakdown of yeast cell membranes. It releases functionally active and solubilized proteins without the need for abrasive methods like glass beads or mechanical treatments with strong reducing agents. With a mild detergent formulation, ReadiUse™ Yeast Cell Lysis Buffer minimizes the reliance on harsh chemical treatments, extreme pH adjustments, or temperature variations, contributing to the preservation of extracted protein integrity. Versatile in its efficacy across various organisms, including Bacillus subtilis, Gram-positive, and Gram-negative bacteria, ReadiUse™ Yeast Cell Lysis Buffer eliminates the need for external disruptive factors, simplifying the cell lysis process. Its applications span molecular biology research, protein purification, analysis, and biomolecular studies, offering a reliable solution for scientific experimentation.<

References

View all 19 references: Citation Explorer

Evaluation of Physicochemical and Microbial Properties of Extracts from Wine Lees Waste of Matelica's Verdicchio and Their Applications in Novel Cosmetic Products.

Authors:

Di Nicolantonio, Lucrezia and Ferrati, Marta and Cristino, Maria and Peregrina, Dolores Vargas and Zannotti, Marco and Vitali, Luca Agostino and Ciancia, Sonia Ilaria and Giovannetti, Rita and Ferraro, Stefano and Zara, Susi and Di Valerio, Valentina and Cataldi, Amelia and Gigliobianco, Maria Rosa and Censi, Roberta and Di Martino, Piera

Journal:

Antioxidants (Basel, Switzerland) (2023)

Cloning and Overexpressing Membrane Proteins Using Pichia pastoris (Komagataella phaffii).

Authors:

Schwob, Magali and Kugler, Valérie and Wagner, Renaud

Journal:

Current protocols (2023): e936

Reliable Approach for Pure Yeast Cell Wall Protein Isolation from Saccharomyces cerevisiae Yeast Cells.

Authors:

Yammine, Marie and Bray, Fabrice and Flament, Stéphanie and Picavet, Antoine and Lacroix, Jean-Marie and Poilpré, Emmanuel and Mouly, Isabelle and Rolando, Christian

Journal:

ACS omega (2022): 29702-29713

Optimised Production and Extraction of Astaxanthin from the Yeast Xanthophyllomyces dendrorhous.

Authors:

Harith, Zuharlida Tuan and de Andrade Lima, Micael and Charalampopoulos, Dimitris and Chatzifragkou, Afroditi

Journal:

Microorganisms (2020)

Effects of farnesol and lyticase on the formation of Candida albicans biofilm.

Authors:

Sachivkina, Nadezhda and Lenchenko, Ekaterina and Blumenkrants, Dmitri and Ibragimova, Alfia and Bazarkina, Olga

Journal:

Veterinary world (2020): 1030-1036

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C)

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Page updated on October 25, 2026