ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilization in laboratory applications. Engineered to simplify the extraction of cell components, including proteins, lipids, nucleic acids, reporter molecules, and small biomolecules, this buffer employs a detergent-based lysis procedure for the gentle breakdown of yeast cell membranes. It releases functionally active and solubilized proteins without the need for abrasive methods like glass beads or mechanical treatments with strong reducing agents. With a mild detergent formulation, ReadiUse™ Yeast Cell Lysis Buffer minimizes the reliance on harsh chemical treatments, extreme pH adjustments, or temperature variations, contributing to the preservation of extracted protein integrity. Versatile in its efficacy across various organisms, including Bacillus subtilis, Gram-positive, and Gram-negative bacteria, ReadiUse™ Yeast Cell Lysis Buffer eliminates the need for external disruptive factors, simplifying the cell lysis process. Its applications span molecular biology research, protein purification, analysis, and biomolecular studies, offering a reliable solution for scientific experimentation.
Evaluation of Physicochemical and Microbial Properties of Extracts from Wine Lees Waste of Matelica's Verdicchio and Their Applications in Novel Cosmetic Products.
Authors:
Di Nicolantonio, Lucrezia and Ferrati, Marta and Cristino, Maria and Peregrina, Dolores Vargas and Zannotti, Marco and Vitali, Luca Agostino and Ciancia, Sonia Ilaria and Giovannetti, Rita and Ferraro, Stefano and Zara, Susi and Di Valerio, Valentina and Cataldi, Amelia and Gigliobianco, Maria Rosa and Censi, Roberta and Di Martino, Piera
Journal:
Antioxidants (Basel, Switzerland) (2023)
Cloning and Overexpressing Membrane Proteins Using Pichia pastoris (Komagataella phaffii).
Authors:
Schwob, Magali and Kugler, Valérie and Wagner, Renaud
Journal:
Current protocols (2023): e936
Reliable Approach for Pure Yeast Cell Wall Protein Isolation from Saccharomyces cerevisiae Yeast Cells.
Authors:
Yammine, Marie and Bray, Fabrice and Flament, Stéphanie and Picavet, Antoine and Lacroix, Jean-Marie and Poilpré, Emmanuel and Mouly, Isabelle and Rolando, Christian
Journal:
ACS omega (2022): 29702-29713
Optimised Production and Extraction of Astaxanthin from the Yeast Xanthophyllomyces dendrorhous.
Authors:
Harith, Zuharlida Tuan and de Andrade Lima, Micael and Charalampopoulos, Dimitris and Chatzifragkou, Afroditi
Journal:
Microorganisms (2020)
Effects of farnesol and lyticase on the formation of Candida albicans biofilm.
Authors:
Sachivkina, Nadezhda and Lenchenko, Ekaterina and Blumenkrants, Dmitri and Ibragimova, Alfia and Bazarkina, Olga