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ROS Brite™ 670 *Optimized for Detecting Reactive Oxygen Species (ROS)*

Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders. ROS Brite™ 670 reagent is a new fluorogenic probe to measure oxidative stress in cells using conventional fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry. The cell-permeant ROS Brite™ 670 reagent is nonfluorescent and produces bright near-infrared fluorescence upon ROS oxidation. The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. It is an excellent alternative to CellROX™ Deep Red Reagent (C10422) for oxidative stress detection (CellROX™ is a trademark of ThermoFisher).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ROS Brite™ 670 *Optimized for Detecting Reactive Oxygen Species (ROS)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM131.778 µL658.892 µL1.318 mL6.589 mL13.178 mL
5 mM26.356 µL131.778 µL263.557 µL1.318 mL2.636 mL
10 mM13.178 µL65.889 µL131.778 µL658.892 µL1.318 mL

Molarity calculator

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Spectrum

Citations

View all 8 citations: Citation Explorer
Complex intestinal and hepatic in vitro barrier models reveal information on uptake and impact of micro-, submicro-and nanoplastics
Authors: Paul, Maxi B and B{\"o}hmert, Linda and Hsiao, I-Lun and Braeuning, Albert and Sieg, Holger
Journal: Environment International (2023): 108172
Environmental light-activated nanozymes for efficient inactivation of harmful algae and associated hemolytic toxin
Authors: Wang, Huibo and Liu, Sidi and Xu, Zhibin and Weng, Xiaoyu and Liao, Changrui and He, Jun and Liu, Liwei and Wang, Yiping and Qu, Junle and Li, Hao and others,
Journal: Chemical Engineering Journal (2023): 145029
CDK4/6 blockade provides an alternative approach for treatment of mismatch-repair deficient tumors
Authors: Salewski, Inken and Henne, Julia and Engster, Leonie and Krone, Paula and Schneider, Bjoern and Redwanz, Caterina and Lemcke, Heiko and Henze, Larissa and Junghanss, Christian and Maletzki, Claudia
Journal: Oncoimmunology (2022): 2094583
Toxin-Enabled “On-Demand” Liposomes for Enhanced Phototherapy to Treat and Protect against Methicillin-Resistant Staphylococcus aureus Infection
Authors: Zhuge, Deli and Chen, Mengchun and Yang, Xuewei and Zhang, Xufei and Yao, Lulu and Li, Li and Wang, Haonan and Chen, Hao and Yin, Qingqing and Tian, Dongyan and others,
Journal: Small (2022): 2203292
Thiol-Mediated Synthesis of Hyaluronic Acid-Epigallocatechin-3-O-Gallate Conjugates for the Formation of Injectable Hydrogels with Free Radical Scavenging Property and Degradation Resistance
Authors: Liu, Chixuan and Bae, Ki Hyun and Yamashita, Atsushi and Chung, Joo Eun and Kurisawa, Motoichi
Journal: Biomacromolecules (2017)

References

View all 91 references: Citation Explorer
Effect of glucocorticoid on production of reactive oxygen species in bone microvascular endothelial cells
Authors: Yang Y, Lou J, Li Z, Sun W, Wang B, Jia Y.
Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2011): 533
Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
Authors: Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.
Journal: Acta Pharmacol Sin (2011): 1266
Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice
Authors: Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.
Journal: Pharm Res (2011): 702
The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: a comparison with the effect of doxorubicin
Authors: Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.
Journal: Toxicol In Vitro (2011): 1712
Coenzyme Q functionalized CdTe/ZnS quantum dots for reactive oxygen species (ROS) imaging
Authors: Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.
Journal: Chemistry (2011): 5262
Page updated on November 5, 2024

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Catalog Number16002
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Physical properties

Molecular weight

758.85

Solvent

DMSO

Spectral properties

Excitation (nm)

651

Emission (nm)

670

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Fluorescence response of ROS Brite&trade; 670&nbsp;to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 640/680 nm.
Fluorescence response of ROS Brite&trade; 670&nbsp;to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 640/680 nm.
Fluorescence response of ROS Brite&trade; 670&nbsp;to different reactive oxygen species in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 640/680 nm.