ROS Brite™ 670 *Optimized for Detecting Reactive Oxygen Species (ROS)*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 758.85 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 651 |
Emission (nm) | 670 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Reactive Oxygen Species (ROS)
Molecular weight 758.85 | Excitation (nm) 651 | Emission (nm) 670 |
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons. ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress (e.g., UV or heat exposure), ROS levels can increase dramatically. This may result in significant damage to cell structures. Cumulatively, this is known as oxidative stress. ROS are also generated by exogenous sources such as ionizing radiation. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in subsequent alteration of membrane lipids, proteins, and nucleic acids. Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events, including atherosclerosis, carcinogenesis, ischemic reperfusion injury, and neurodegenerative disorders. ROS Brite™ 670 reagent is a new fluorogenic probe to measure oxidative stress in cells using conventional fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry. The cell-permeant ROS Brite™ 670 reagent is nonfluorescent and produces bright near-infrared fluorescence upon ROS oxidation. The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. It is an excellent alternative to CellROX™ Deep Red Reagent (C10422) for oxidative stress detection (CellROX™ is a trademark of ThermoFisher).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ROS Brite™ 670 *Optimized for Detecting Reactive Oxygen Species (ROS)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 131.778 µL | 658.892 µL | 1.318 mL | 6.589 mL | 13.178 mL |
5 mM | 26.356 µL | 131.778 µL | 263.557 µL | 1.318 mL | 2.636 mL |
10 mM | 13.178 µL | 65.889 µL | 131.778 µL | 658.892 µL | 1.318 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Images
Citations
View all 8 citations: Citation Explorer
Complex intestinal and hepatic in vitro barrier models reveal information on uptake and impact of micro-, submicro-and nanoplastics
Authors: Paul, Maxi B and B{\"o}hmert, Linda and Hsiao, I-Lun and Braeuning, Albert and Sieg, Holger
Journal: Environment International (2023): 108172
Authors: Paul, Maxi B and B{\"o}hmert, Linda and Hsiao, I-Lun and Braeuning, Albert and Sieg, Holger
Journal: Environment International (2023): 108172
Environmental light-activated nanozymes for efficient inactivation of harmful algae and associated hemolytic toxin
Authors: Wang, Huibo and Liu, Sidi and Xu, Zhibin and Weng, Xiaoyu and Liao, Changrui and He, Jun and Liu, Liwei and Wang, Yiping and Qu, Junle and Li, Hao and others,
Journal: Chemical Engineering Journal (2023): 145029
Authors: Wang, Huibo and Liu, Sidi and Xu, Zhibin and Weng, Xiaoyu and Liao, Changrui and He, Jun and Liu, Liwei and Wang, Yiping and Qu, Junle and Li, Hao and others,
Journal: Chemical Engineering Journal (2023): 145029
Toxin-Enabled “On-Demand” Liposomes for Enhanced Phototherapy to Treat and Protect against Methicillin-Resistant Staphylococcus aureus Infection
Authors: Zhuge, Deli and Chen, Mengchun and Yang, Xuewei and Zhang, Xufei and Yao, Lulu and Li, Li and Wang, Haonan and Chen, Hao and Yin, Qingqing and Tian, Dongyan and others,
Journal: Small (2022): 2203292
Authors: Zhuge, Deli and Chen, Mengchun and Yang, Xuewei and Zhang, Xufei and Yao, Lulu and Li, Li and Wang, Haonan and Chen, Hao and Yin, Qingqing and Tian, Dongyan and others,
Journal: Small (2022): 2203292
CDK4/6 blockade provides an alternative approach for treatment of mismatch-repair deficient tumors
Authors: Salewski, Inken and Henne, Julia and Engster, Leonie and Krone, Paula and Schneider, Bjoern and Redwanz, Caterina and Lemcke, Heiko and Henze, Larissa and Junghanss, Christian and Maletzki, Claudia
Journal: Oncoimmunology (2022): 2094583
Authors: Salewski, Inken and Henne, Julia and Engster, Leonie and Krone, Paula and Schneider, Bjoern and Redwanz, Caterina and Lemcke, Heiko and Henze, Larissa and Junghanss, Christian and Maletzki, Claudia
Journal: Oncoimmunology (2022): 2094583
Thiol-Mediated Synthesis of Hyaluronic Acid-Epigallocatechin-3-O-Gallate Conjugates for the Formation of Injectable Hydrogels with Free Radical Scavenging Property and Degradation Resistance
Authors: Liu, Chixuan and Bae, Ki Hyun and Yamashita, Atsushi and Chung, Joo Eun and Kurisawa, Motoichi
Journal: Biomacromolecules (2017)
Authors: Liu, Chixuan and Bae, Ki Hyun and Yamashita, Atsushi and Chung, Joo Eun and Kurisawa, Motoichi
Journal: Biomacromolecules (2017)
Transient receptor potential melastatin 8 ion channel in macrophages modulates colitis through a balance-shift in TNF-alpha and interleukin-10 production
Authors: Khalil, M and Babes, A and Lakra, R and Försch, S and Reeh, PW and Wirtz, S and Becker, C and Neurath, MF and Engel, MA
Journal: Mucosal immunology (2016)
Authors: Khalil, M and Babes, A and Lakra, R and Försch, S and Reeh, PW and Wirtz, S and Becker, C and Neurath, MF and Engel, MA
Journal: Mucosal immunology (2016)
VEGFR2 signaling prevents colorectal cancer cell senescence to promote tumorigenesis in mice with colitis
Authors: Foersch, Sebastian and Sperka, Tobias and Lindner, Christina and Taut, Astrid and Rudolph, Karl L and Breier, Georg and Boxberger, Frank and Rau, Tilman T and Hartmann, Arndt and Stürzl, Michael and others, undefined
Journal: Gastroenterology (2015): 177--189
Authors: Foersch, Sebastian and Sperka, Tobias and Lindner, Christina and Taut, Astrid and Rudolph, Karl L and Breier, Georg and Boxberger, Frank and Rau, Tilman T and Hartmann, Arndt and Stürzl, Michael and others, undefined
Journal: Gastroenterology (2015): 177--189
Oxidative Stress--An Update and Insight in the Romanian Family Physician’s Adoption of the Concept
Authors: Berghea, Florian and Berghea, Camelia Elena and Abobului, Mihai
Journal: Internal Medicine : 11--15
Authors: Berghea, Florian and Berghea, Camelia Elena and Abobului, Mihai
Journal: Internal Medicine : 11--15
References
View all 91 references: Citation Explorer
Lipoxin A inhibits porphyromonas gingivalis-induced aggregation and reactive oxygen species production by modulating neutrophil-platelet interaction and CD11b expression
Authors: Borgeson E, Lonn J, Bergstrom I, Brodin VP, Ramstrom S, Nayeri F, Sarndahl E, Bengtsson T.
Journal: Infect Immun (2011): 1489
Authors: Borgeson E, Lonn J, Bergstrom I, Brodin VP, Ramstrom S, Nayeri F, Sarndahl E, Bengtsson T.
Journal: Infect Immun (2011): 1489
Role of hyaluronan and CD44 in reactive oxygen species-induced mucus hypersecretion
Authors: Yu H, Li Q, Zhou X, Kolosov VP, Perelman JM.
Journal: Mol Cell Biochem (2011): 65
Authors: Yu H, Li Q, Zhou X, Kolosov VP, Perelman JM.
Journal: Mol Cell Biochem (2011): 65
Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells
Authors: Hossain MZ, Kleve MG.
Journal: Int J Nanomedicine (2011): 1475
Authors: Hossain MZ, Kleve MG.
Journal: Int J Nanomedicine (2011): 1475
Effect of glucocorticoid on production of reactive oxygen species in bone microvascular endothelial cells
Authors: Yang Y, Lou J, Li Z, Sun W, Wang B, Jia Y.
Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2011): 533
Authors: Yang Y, Lou J, Li Z, Sun W, Wang B, Jia Y.
Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2011): 533
Regulation of cyclooxygenase-2 and cytosolic phospholipase A2 gene expression by lipopolysaccharide through the RNA-binding protein HuR: involvement of NADPH oxidase, reactive oxygen species and mitogen-activated protein kinases
Authors: Lin WN, Lin CC, Cheng HY, Yang CM.
Journal: Br J Pharmacol (2011): 1691
Authors: Lin WN, Lin CC, Cheng HY, Yang CM.
Journal: Br J Pharmacol (2011): 1691
Effects of hypothermic storage on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and plasma membrane integrity in striped bass (Morone saxatilis) sperm
Authors: Guthrie HD, Welch GR, Theisen DD, Woods LC, 3rd.
Journal: Theriogenology (2011): 951
Authors: Guthrie HD, Welch GR, Theisen DD, Woods LC, 3rd.
Journal: Theriogenology (2011): 951
Coenzyme Q functionalized CdTe/ZnS quantum dots for reactive oxygen species (ROS) imaging
Authors: Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.
Journal: Chemistry (2011): 5262
Authors: Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.
Journal: Chemistry (2011): 5262
The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: a comparison with the effect of doxorubicin
Authors: Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.
Journal: Toxicol In Vitro (2011): 1712
Authors: Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.
Journal: Toxicol In Vitro (2011): 1712
Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice
Authors: Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.
Journal: Pharm Res (2011): 702
Authors: Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.
Journal: Pharm Res (2011): 702
Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
Authors: Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.
Journal: Acta Pharmacol Sin (2011): 1266
Authors: Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.
Journal: Acta Pharmacol Sin (2011): 1266
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells