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TF3-DEVD-FMK

TF3-DEVD-FMK is a superior replacement for 5-TAMRA-DEVD-FMK, i.e., 5-carboxytetramethylrhdamine-Asp-Glu-Val-Asp-fluoromethylketone since it can be better excited at 555 nm. TF3 dyes has identical spectral properties to Cy3 with significantly enhanced photostability. TF3-DEVD-FMK binds irreversibly to active caspase 3/7 in stimulated cells. The fluorescent intensity of the TF3-DEVD-FMK signal is proportional to the amount of active caspase 3/7 and can be easily detected by fluorescence microscopy, flow cytometer, or fluorescent microplate reader.

Example protocol

AT A GLANCE

Important notes

It is important to store at <-15 °C and should be stored in cool, dark place.

It can be used within 12 months from the date of receipt. 

SAMPLE EXPERIMENTAL PROTOCOL

Following protocol only provides a guideline, and should be modified according to your specific needs.

General Solution Caspase Assays Using AMC, AFC, pNA, R110 and ProRed Substrates

  1. Prepare a 10 mM stock solution in DMSO.

  2. Prepare a 2X caspase substrate (50 µM) assay solution as the following: 50 µL substrate stock solution, 100 µL DTT (1M), 400 µL EDTA (100 mM), 10 mL Tris Buffer (20 mM), pH =7.4.

  3. Mix equal volume of the caspase standards or samples with 2X caspase substrate assay solution, and incubate the solutions at room temperature for at least 1 hour.

  4. Monitor the fluorescence using a fluorescence microplate reader, or absorbance using an absorbance microplate reader.

Cell Caspase Assays Using Cell-Permeable FMK Caspase Probes

  1. Prepare a 2-5 mM stock solution in DMSO.

  2. Treat cells as desired.

  3. Prepare a 2X permeable caspase substrate (20 µM) assay solution by diluting the DMSO stock solution (from Step 2.1) in Hanks with 20 mM Hepes buffer (HHBS).

  4. Mix equal volume of the treated cells with 2X caspase substrate assay solution (from Step 2.3), and incubate the cells in a 37°C, 5% CO2 incubator for at least1 hour.

  5. Wash the cells with HHBS for at least once.

  6. Monitor the fluorescence intensity by a flow cytometer, a fluorescence microscope or a fluorescence microplate reader.

Cell Caspase Assays Using Cell-Permeable FMK Caspase Probes (For #13470-13476 only)

  1. Prepare a 250X stock solution by adding 50 µL DMSO into the vial.

  2. Treat cells as desired.

  3. Add 250 X DMSO stock solution into the cell solution at a 1:250 ratio (such as 2 µL to 500 µL cells), and incubate the cells in a 37°C, 5% CO2 incubator for 1 hour.

  4. Wash the cells with HHBS for at least once.

  5. Monitor the fluorescence intensity by flow cytometer, fluorescence microscopy or fluorescent microplate reader.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of TF3-DEVD-FMK to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM102.56 µL512.799 µL1.026 mL5.128 mL10.256 mL
5 mM20.512 µL102.56 µL205.12 µL1.026 mL2.051 mL
10 mM10.256 µL51.28 µL102.56 µL512.799 µL1.026 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum

Citations

View all 30 citations: Citation Explorer
IL-21R-STAT3 signalling initiates a differentiation program in uterine tissue-resident NK cells to support pregnancy
Authors: Han, Mengwei and Hu, Luni and Wu, Di and Zhang, Yime and Li, Peng and Zhao, Xingyu and Zeng, Yanyu and Ren, Guanqun and Hou, Zhiyuan and Pang, Yanli and others,
Journal: Nature Communications (2023): 7109
The Dietary Flavonoid Fisetin Causes Cell Cycle Arrest, Caspase-Dependent Apoptosis, and Enhanced Cytotoxicity of Chemotherapeutic Drugs in Triple-Negative Breast Cancer Cells
Authors: Smith, M. L., Murphy, K., Doucette, C. D., Greenshields, A. L., Hoskin, D. W.
Journal: J Cell Biochem (2016): 1913-25
Contribution of membrane progesterone receptor alpha to the induction of progesterone-mediated apoptosis associated with mitochondrial membrane disruption and caspase cascade activation in Jurkat cell lines
Authors: Kon, A., Yuan, B., Hanazawa, T., Kikuchi, H., Sato, M., Furutani, R., Takagi, N., Toyoda, H.
Journal: Oncol Rep (2013): 1965-70
Pan-caspase inhibition suppresses polyethylene particle-induced osteolysis
Authors: L, undefined and graeber, S., Jaeckel, S., Loer, F., Wedemeyer, C., Hilken, G., Canbay, A., Totsch, M., von Knoch, M.
Journal: Apoptosis (2009): 173-81
Inhibition of Caspase-Like Activities Prevents the Appearance of Reactive Oxygen Species and Dark-Induced Apoptosis in the Unicellular Chlorophyte Dunaliella Tertiolecta(1)
Authors: Segovia, M., Berges, J. A.
Journal: J Phycol (2009): 1116-26

References

View all 26 references: Citation Explorer
Suppression of human T cell proliferation by the caspase inhibitors, z-VAD-FMK and z-IETD-FMK is independent of their caspase inhibition properties
Authors: Lawrence CP, Chow SC.
Journal: Toxicol Appl Pharmacol. (2012)
Inhibition of elicitation of allergic contact dermatitis by topical use of Z-VAD-FMK, a broad caspase inhibitor: experiment in mice
Authors: Li YY, Yan CL.
Journal: Zhonghua Yi Xue Za Zhi (2012): 1992
Structure of human caspase-6 in complex with Z-VAD-FMK: New peptide binding mode observed for the non-canonical caspase conformation
Authors: Muller I, Lamers MB, Ritchie AJ, Dominguez C, Munoz-Sanjuan I, Kiselyov A.
Journal: Bioorg Med Chem Lett (2011): 5244
Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss
Authors: Abaamrane L, Raffin F, Schmerber S, Sendowski I.
Journal: Eur Arch Otorhinolaryngol (2011): 987
Plasmodium falciparum metacaspase PfMCA-1 triggers a z-VAD-fmk inhibitable protease to promote cell death
Authors: Meslin B, Beavogui AH, Fasel N, Picot S.
Journal: PLoS One (2011): e23867
Page updated on November 1, 2024

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Catalog Number13474
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Physical properties

Molecular weight

975.04

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.179

Extinction coefficient (cm -1 M -1)

750001

Excitation (nm)

553

Emission (nm)

578

Quantum yield

0.11

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200