Name: TF3-DEVD-FMK
Brand: AAT Bioquest
SKU: 13474
Price: 115 USD
Availability: InStock

TF3-DEVD-FMK

TF3-DEVD-FMK is a superior replacement for 5-TAMRA-DEVD-FMK, i.e., 5-carboxytetramethylrhdamine-Asp-Glu-Val-Asp-fluoromethylketone since it can be better excited at 555 nm. TF3 dyes has identical spectral properties to Cy3 with significantly enhanced photostability. TF3-DEVD-FMK binds irreversibly to active caspase 3/7 in stimulated cells. The fluorescent intensity of the TF3-DEVD-FMK signal is proportional to the amount of active caspase 3/7 and can be easily detected by fluorescence microscopy, flow cytometer, or fluorescent microplate reader.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13474	25 Tests	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of TF3-DEVD-FMK to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	102.56 µL	512.799 µL	1.026 mL	5.128 mL	10.256 mL
5 mM	20.512 µL	102.56 µL	205.12 µL	1.026 mL	2.051 mL
10 mM	10.256 µL	51.28 µL	102.56 µL	512.799 µL	1.026 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	975.04 g/mol	=	mL	x	mM	=	mmol

Citations

View all 30 citations: Citation Explorer

IL-21R-STAT3 signalling initiates a differentiation program in uterine tissue-resident NK cells to support pregnancy

Authors:

Han, Mengwei and Hu, Luni and Wu, Di and Zhang, Yime and Li, Peng and Zhao, Xingyu and Zeng, Yanyu and Ren, Guanqun and Hou, Zhiyuan and Pang, Yanli and others,

Journal:

Nature Communications (2023): 7109

The Dietary Flavonoid Fisetin Causes Cell Cycle Arrest, Caspase-Dependent Apoptosis, and Enhanced Cytotoxicity of Chemotherapeutic Drugs in Triple-Negative Breast Cancer Cells

Authors:

Smith, M. L., Murphy, K., Doucette, C. D., Greenshields, A. L., Hoskin, D. W.

Journal:

J Cell Biochem (2016): 1913-25

Contribution of membrane progesterone receptor alpha to the induction of progesterone-mediated apoptosis associated with mitochondrial membrane disruption and caspase cascade activation in Jurkat cell lines

Authors:

Kon, A., Yuan, B., Hanazawa, T., Kikuchi, H., Sato, M., Furutani, R., Takagi, N., Toyoda, H.

Journal:

Oncol Rep (2013): 1965-70

Pan-caspase inhibition suppresses polyethylene particle-induced osteolysis

Authors:

L, undefined and graeber, S., Jaeckel, S., Loer, F., Wedemeyer, C., Hilken, G., Canbay, A., Totsch, M., von Knoch, M.

Journal:

Apoptosis (2009): 173-81

Inhibition of Caspase-Like Activities Prevents the Appearance of Reactive Oxygen Species and Dark-Induced Apoptosis in the Unicellular Chlorophyte Dunaliella Tertiolecta(1)

Authors:

Segovia, M., Berges, J. A.

Journal:

J Phycol (2009): 1116-26

References

View all 26 references: Citation Explorer

Suppression of human T cell proliferation by the caspase inhibitors, z-VAD-FMK and z-IETD-FMK is independent of their caspase inhibition properties

Authors:

Lawrence CP, Chow SC.

Journal:

Toxicol Appl Pharmacol. (2012)

Inhibition of elicitation of allergic contact dermatitis by topical use of Z-VAD-FMK, a broad caspase inhibitor: experiment in mice

Authors:

Li YY, Yan CL.

Journal:

Zhonghua Yi Xue Za Zhi (2012): 1992

Structure of human caspase-6 in complex with Z-VAD-FMK: New peptide binding mode observed for the non-canonical caspase conformation

Authors:

Muller I, Lamers MB, Ritchie AJ, Dominguez C, Munoz-Sanjuan I, Kiselyov A.

Journal:

Bioorg Med Chem Lett (2011): 5244

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Authors:

Abaamrane L, Raffin F, Schmerber S, Sendowski I.

Journal:

Eur Arch Otorhinolaryngol (2011): 987

Plasmodium falciparum metacaspase PfMCA-1 triggers a z-VAD-fmk inhibitable protease to promote cell death

Authors:

Meslin B, Beavogui AH, Fasel N, Picot S.

Journal:

PLoS One (2011): e23867

Physical properties

Molecular weight	975.04
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.179
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	553
Emission (nm)	578
Quantum yield	0.1 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 12, 2025