Tide Quencher™ 5.1WS alkyne [TQ5.1WS alkyne]
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 989.06 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 5.1WS amine [TQ5.1WS amine] |
Tide Quencher™ 5.1WS maleimide [TQ5.1WS maleimide] |
Tide Quencher™ 5.1WS acid [TQ5.1WS acid] |
Tide Quencher™ 5.1WS azide [TQ5.1WS azide] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 989.06 |
Tide Quencher™ 5.1WS (TQ5.1WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy5, Cy5.5, Alexa Fluor® 647, Alexa Fluor® 647, iFluor® 647 and iFluor® 680. It is an improved version of TQ5 and QSY 21 and BHQ3). TQ5.1WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency. Tide Quencher™ 5.1WS Alkyne is an excellent building block for preparing TQ5.1WS-labeled probes from azido-modified oligonucleotides (including picolyl azide-modified oligonucleotides via the well-known click chemistry. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ5.1WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ5.1WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 5.1WS alkyne [TQ5.1WS alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 101.106 µL | 505.531 µL | 1.011 mL | 5.055 mL | 10.111 mL |
5 mM | 20.221 µL | 101.106 µL | 202.212 µL | 1.011 mL | 2.022 mL |
10 mM | 10.111 µL | 50.553 µL | 101.106 µL | 505.531 µL | 1.011 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Figure 1. TQ5.1WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency. Tide Quencher™ 5.1WS Alkyne is an excellent building block for preparing TQ5.1WS-labeled probes from azido-modified oligonucleotides (including picolyl azide-modified oligonucleotides via the well-known click chemistry.
References
View all 40 references: Citation Explorer
Synthesis of Cy5-Labelled C5-Alkynyl-modified cytidine triphosphates via Sonogashira coupling for DNA labelling.
Authors: Kuznetsova, Viktoriya E and Shershov, Valeriy E and Guseinov, Teimur O and Miftakhov, Rinat A and Solyev, Pavel N and Novikov, Roman A and Levashova, Anna I and Zasedatelev, Alexander S and Lapa, Sergey A and Chudinov, Alexander V
Journal: Bioorganic chemistry (2023): 106315
Authors: Kuznetsova, Viktoriya E and Shershov, Valeriy E and Guseinov, Teimur O and Miftakhov, Rinat A and Solyev, Pavel N and Novikov, Roman A and Levashova, Anna I and Zasedatelev, Alexander S and Lapa, Sergey A and Chudinov, Alexander V
Journal: Bioorganic chemistry (2023): 106315
DNA-Programmed Lipid Nanoreactors for Synthesis of Carbohydrate Mimetics by Fusion of Aqueous Sub-attoliter Compartments.
Authors: Tian, Xinwei and Risgaard, Nikolaj Alexander and Löffler, Philipp M G and Vogel, Stefan
Journal: Journal of the American Chemical Society (2023)
Authors: Tian, Xinwei and Risgaard, Nikolaj Alexander and Löffler, Philipp M G and Vogel, Stefan
Journal: Journal of the American Chemical Society (2023)
Hybridization-Sensitive Fluorescent Probes for DNA and RNA by a Modular "Click" Approach.
Authors: Gebhard, Julian and Hirsch, Lara and Schwechheimer, Christian and Wagenknecht, Hans-Achim
Journal: Bioconjugate chemistry (2022): 1634-1642
Authors: Gebhard, Julian and Hirsch, Lara and Schwechheimer, Christian and Wagenknecht, Hans-Achim
Journal: Bioconjugate chemistry (2022): 1634-1642
Orthogonal Covalent Entrapment of Cargo into Biodegradable Polymeric Micelles via Native Chemical Ligation.
Authors: Hebels, Erik R and Bindt, Felix and Walther, Johanna and van Geijn, Michiel and Weterings, Jimmy and Hu, Qizhi and Colombo, Claudio and Liskamp, Rob and Rijcken, Cristianne and Hennink, Wim E and Vermonden, Tina
Journal: Biomacromolecules (2022)
Authors: Hebels, Erik R and Bindt, Felix and Walther, Johanna and van Geijn, Michiel and Weterings, Jimmy and Hu, Qizhi and Colombo, Claudio and Liskamp, Rob and Rijcken, Cristianne and Hennink, Wim E and Vermonden, Tina
Journal: Biomacromolecules (2022)
Chemical Synthesis of a Functional Fluorescent-Tagged α-Bungarotoxin.
Authors: Brun, Oliver and Zoukimian, Claude and Oliveira-Mendes, Barbara and Montnach, Jérôme and Lauzier, Benjamin and Ronjat, Michel and Béroud, Rémy and Lesage, Frédéric and Boturyn, Didier and De Waard, Michel
Journal: Toxins (2022)
Authors: Brun, Oliver and Zoukimian, Claude and Oliveira-Mendes, Barbara and Montnach, Jérôme and Lauzier, Benjamin and Ronjat, Michel and Béroud, Rémy and Lesage, Frédéric and Boturyn, Didier and De Waard, Michel
Journal: Toxins (2022)
Efficient Labeling of Nanocellulose for High-Resolution Fluorescence Microscopy Applications.
Authors: Babi, Mouhanad and Fatona, Ayodele and Li, Xiang and Cerson, Christine and Jarvis, Victoria M and Abitbol, Tiffany and Moran-Mirabal, Jose M
Journal: Biomacromolecules (2022): 1981-1994
Authors: Babi, Mouhanad and Fatona, Ayodele and Li, Xiang and Cerson, Christine and Jarvis, Victoria M and Abitbol, Tiffany and Moran-Mirabal, Jose M
Journal: Biomacromolecules (2022): 1981-1994
Multiscale imaging of therapeutic anti-PD-L1 antibody localization using molecularly defined imaging agents.
Authors: Hagemans, Iris M and Wierstra, Peter J and Steuten, Kas and Molkenboer-Kuenen, Janneke D M and van Dalen, Duco and Ter Beest, Martin and van der Schoot, Johan M S and Ilina, Olga and Gotthardt, Martin and Figdor, Carl G and Scheeren, Ferenc A and Heskamp, Sandra and Verdoes, Martijn
Journal: Journal of nanobiotechnology (2022): 64
Authors: Hagemans, Iris M and Wierstra, Peter J and Steuten, Kas and Molkenboer-Kuenen, Janneke D M and van Dalen, Duco and Ter Beest, Martin and van der Schoot, Johan M S and Ilina, Olga and Gotthardt, Martin and Figdor, Carl G and Scheeren, Ferenc A and Heskamp, Sandra and Verdoes, Martijn
Journal: Journal of nanobiotechnology (2022): 64
Click Chemistry-Enabled Conjugation Strategy for Producing Dibenzodiazepinone-Type Fluorescent Probes To Target M2 Acetylcholine Receptors.
Authors: Yang, Hongrong and Micovic, Nicholas and Monaghan, James R and Clark, Heather A
Journal: Bioconjugate chemistry (2022): 2223-2233
Authors: Yang, Hongrong and Micovic, Nicholas and Monaghan, James R and Clark, Heather A
Journal: Bioconjugate chemistry (2022): 2223-2233
Bacteria-Mediated Intracellular Click Reaction for Drug Enrichment and Selective Apoptosis of Drug-Resistant Tumor Cells.
Authors: Gao, Zhiqiang and Zhang, Endong and Zhao, Hao and Xia, Shengpeng and Bai, Haotian and Huang, Yiming and Lv, Fengting and Liu, Libing and Wang, Shu
Journal: ACS applied materials & interfaces (2022): 12106-12115
Authors: Gao, Zhiqiang and Zhang, Endong and Zhao, Hao and Xia, Shengpeng and Bai, Haotian and Huang, Yiming and Lv, Fengting and Liu, Libing and Wang, Shu
Journal: ACS applied materials & interfaces (2022): 12106-12115
Bioorthogonal chemistry in metal clusters: a general strategy for the construction of multifunctional probes for bioimaging in living cells and in vivo.
Authors: Chen, Xueqian and Zhang, Yong and Yuan, Qing and Li, Mingrui and Bian, Yongning and Su, Dongdong and Gao, Xueyun
Journal: Journal of materials chemistry. B (2021): 6614-6622
Authors: Chen, Xueqian and Zhang, Yong and Yuan, Qing and Li, Mingrui and Bian, Yongning and Su, Dongdong and Gao, Xueyun
Journal: Journal of materials chemistry. B (2021): 6614-6622