Tide Quencher™ 7.2WS acid [TQ7.2WS acid]
Additional ordering information
|Shipping||Standard overnight for United States, inquire for international|
Storage, safety and handling
|H-phrase||H303, H313, H333|
|Intended use||Research Use Only (RUO)|
|R-phrase||R20, R21, R22|
|Storage||Freeze (< -15 °C); Minimize light exposure|
|Tide Quencher™ 7.2WS amine [TQ7.2WS amine]|
|Tide Quencher™ 7.2WS maleimide [TQ7.2WS maleimide]|
|Tide Quencher™ 7.2WS azide [TQ7.2WS azide]|
|Tide Quencher™ 7.2WS alkyne [TQ7.2WS alkyne]|
See also: Tide Quencher Dyes
Tide Quencher™ 7.2WS (TQ7.2WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.2WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.2WS acid is primarily used for labeling amino-modified oligonucleotides and peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.2WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ7.2WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 7.2WS acid [TQ7.2WS acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
|0.1 mg||0.5 mg||1 mg||5 mg||10 mg|
|1 mM||102.245 µL||511.227 µL||1.022 mL||5.112 mL||10.225 mL|
|5 mM||20.449 µL||102.245 µL||204.491 µL||1.022 mL||2.045 mL|
|10 mM||10.225 µL||51.123 µL||102.245 µL||511.227 µL||1.022 mL|
Enter any two values (mass, volume, concentration) to calculate the third.
|Mass (Calculate)||Molecular weight||Volume (Calculate)||Concentration (Calculate)||Moles|