XFD488 aldehyde Same Structure to Alexa Fluor™ 488 aldehyde

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	750.75
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.30
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	490
Emission (nm)	520
Quantum yield	0.92¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

750.75

Correction Factor (260 nm)

0.30

Correction Factor (280 nm)

0.11

Extinction coefficient (cm ^-1 M ^-1)

71000

Excitation (nm)

490

Emission (nm)

520

Quantum yield

0.92¹

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). XFD488 aldehyde contains the fluorophore of XFD488. It is a reactive green fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated XFD488 dye can be easily detected by a common fluorescence instrument under FITC channel.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	133.2 µL	666.001 µL	1.332 mL	6.66 mL	13.32 mL
5 mM	26.64 µL	133.2 µL	266.4 µL	1.332 mL	2.664 mL
10 mM	13.32 µL	66.6 µL	133.2 µL	666.001 µL	1.332 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Correction Factor (260 nm)	0.30
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	490
Emission (nm)	520
Quantum yield	0.92¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 azide Same Structure to Alexa Fluor™ 488 azide	490	520	71000	0.92¹	0.30	0.11
XFD488 alkyne Same Structure to Alexa Fluor™ 488 alkyne	490	520	71000	0.92¹	0.30	0.11
XFD488 Hydroxylamine Same Structure to Alexa Fluor™ 488 Hydroxylamine	490	520	71000	0.92¹	0.30	0.11
XFD488 amine Same Structure to Alexa Fluor™ 488 amine	490	520	71000	0.92¹	0.30	0.11
XFD488 Phalloidin XFD488 Same Structure to Alexa Fluor™ 488	499	520	73000	0.92¹	0.3	0.11
XFD488 acid Same Structure to Alexa Fluor™ 488 acid	490	520	71000	0.92¹	0.30	0.11
XFD488 tetrazine Same Structure to Alexa Fluor™ 488 tetrazine	490	520	71000	0.92¹	0.30	0.11
XFD488 TCO	499	520	73000	0.92¹	0.3	0.11
Fluorescein aldehyde [5-FAM aldehyde]	493	517	83000	-	0.32	0.178
Rhodamine aldehyde [5-TAMRA aldehyde]	552	578	90000	-	0.32	0.178
Cy3 aldehyde	555	569	150000¹	0.15¹	0.07	0.073
Cy5 aldehyde	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
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Images

Figure 1. Chemical structure for XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*.

References

View all 10 references: Citation Explorer

Optimization of 3-aminopropyltriethoxysilane functionalization on silicon nitride surface for biomolecule immobilization.
Authors: Saengdee, Pawasuth and Promptmas, Chamras and Thanapitak, Surachoke and Srisuwan, Awirut and Pankiew, Apirak and Thornyanadacha, Nutthaphat and Chaisriratanakul, Woraphan and Chaowicharat, Ekalak and Jeamsaksiri, Wutthinan
Journal: Talanta (2020): 120305

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments.
Authors: Grate, Jay W and Mo, Kai-For and Shin, Yongsoon and Vasdekis, Andreas and Warner, Marvin G and Kelly, Ryan T and Orr, Galya and Hu, Dehong and Dehoff, Karl J and Brockman, Fred J and Wilkins, Michael J
Journal: Bioconjugate chemistry (2015): 593-601

Simultaneous visualization and cell-specific confirmation of RNA and protein in the mouse retina.
Authors: Stempel, Andrew J and Morgans, Catherine W and Stout, J Timothy and Appukuttan, Binoy
Journal: Molecular vision (2014): 1366-73

Amine-reactive pyridylhydrazone-based PEG reagents for pH-reversible PEI polyplex shielding.
Authors: Fella, Carolin and Walker, Greg F and Ogris, Manfred and Wagner, Ernst
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2008): 309-20

Raman nanoparticle probes for antibody-based protein detection in tissues.
Authors: Lutz, Barry and Dentinger, Claire and Sun, Lei and Nguyen, Lienchi and Zhang, Jingwu and Chmura, Aj and Allen, April and Chan, Selena and Knudsen, Beatrice
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2008): 371-9

Rapid oxime and hydrazone ligations with aromatic aldehydes for biomolecular labeling.
Authors: Dirksen, Anouk and Dawson, Philip E
Journal: Bioconjugate chemistry (2008): 2543-8

Use of anti-fluorophore antibody to achieve high-sensitivity immunolocalizations of transporters and ion channels.
Authors: Coleman, Richard A and Liu, Jie and Wade, James B
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2006): 817-27

Oligonucleotide-arrayed TFT photosensor applicable for DNA chip technology.
Authors: Tanaka, Tsuyoshi and Hatakeyama, Keiichi and Sawaguchi, Masahiro and Iwadate, Akihito and Mizutani, Yasushi and Sasaki, Kazuhiro and Tateishi, Naofumi and Takeyama, Haruko and Matsunaga, Tadashi
Journal: Biotechnology and bioengineering (2006): 22-8

Acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils.
Authors: Amraei, Mohammad and Jia, Zongjian and Reboul, Pascal and Nabi, Ivan R
Journal: The Journal of biological chemistry (2003): 38935-41

Lipopolysaccharide (LPS) labeled with Alexa 488 hydrazide as a novel probe for LPS binding studies.
Authors: Triantafilou, K and Triantafilou, M and Fernandez, N
Journal: Cytometry (2000): 316-20