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XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*

Chemical structure for XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*.
Chemical structure for XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*.
Ordering information
Price ()
Catalog Number9015
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight750.75
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)499
Emission (nm)520
Quantum yield0.921
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Related products
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*
XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide*
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester*
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester*
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*
XFD350 C5 Maleimide *Same Structure to Alexa Fluor™ 350 C5 Maleimide*
XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide*
XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide*
XFD350 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350*
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*
XFD488 tyramide reagent *Same Structure to Alexa Fluor™ 488 tyramide*
XFD546 tyramide reagent *Same Structure to Alexa Fluor™ 546 tyramide*
XFD594 tyramide reagent *Same Structure to Alexa Fluor™ 594 tyramide*
XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488*
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester*
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester*
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*
XFD647 maleimide *C2, XFD647 Same Structure to Alexa Fluor™ 647*
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*
XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*
XFD350 tyramide reagent *Same Structure to Alexa Fluor™ 350 tyramide*
XFD568 tyramide reagent *Same Structure to Alexa Fluor™ 568 tyramide*
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*
XFD™488-dUTP *1 mM in Tris Buffer (pH 7.5)*
XFD514 acid
XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester*
XFD514 tyramide
XFD532 tyramide
XFD647 Azide
XFD647 Alkyne
Show More (45)

OverviewpdfSDSpdfProtocol


Molecular weight
750.75
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)
499
Emission (nm)
520
Quantum yield
0.921
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). XFD488 aldehyde contains the fluorophore of XFD488. It is a reactive green fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated XFD488 dye can be easily detected by a common fluorescence instrument under FITC channel.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM133.2 µL666.001 µL1.332 mL6.66 mL13.32 mL
5 mM26.64 µL133.2 µL266.4 µL1.332 mL2.664 mL
10 mM13.32 µL66.6 µL133.2 µL666.001 µL1.332 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)73000
Excitation (nm)499
Emission (nm)520
Quantum yield0.921

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide*499520730000.921--
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne*499520730000.921--
XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine*499520730000.921--
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*499520730000.921--
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*499520730000.9210.30.11
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*499520730000.921--
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*499520730000.921--
XFD488 TCO499520730000.9210.30.11
Fluorescein aldehyde [5-FAM aldehyde]49351783000-0.320.178
Rhodamine aldehyde [5-TAMRA aldehyde]55257890000-0.320.178
Cy3 aldehyde55556915000010.1510.070.073
Cy5 aldehyde65167025000010.271, 0.420.020.03
Show More (13)

References


View all 10 references: Citation Explorer
Optimization of 3-aminopropyltriethoxysilane functionalization on silicon nitride surface for biomolecule immobilization.
Authors: Saengdee, Pawasuth and Promptmas, Chamras and Thanapitak, Surachoke and Srisuwan, Awirut and Pankiew, Apirak and Thornyanadacha, Nutthaphat and Chaisriratanakul, Woraphan and Chaowicharat, Ekalak and Jeamsaksiri, Wutthinan
Journal: Talanta (2020): 120305
Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments.
Authors: Grate, Jay W and Mo, Kai-For and Shin, Yongsoon and Vasdekis, Andreas and Warner, Marvin G and Kelly, Ryan T and Orr, Galya and Hu, Dehong and Dehoff, Karl J and Brockman, Fred J and Wilkins, Michael J
Journal: Bioconjugate chemistry (2015): 593-601
Simultaneous visualization and cell-specific confirmation of RNA and protein in the mouse retina.
Authors: Stempel, Andrew J and Morgans, Catherine W and Stout, J Timothy and Appukuttan, Binoy
Journal: Molecular vision (2014): 1366-73
Amine-reactive pyridylhydrazone-based PEG reagents for pH-reversible PEI polyplex shielding.
Authors: Fella, Carolin and Walker, Greg F and Ogris, Manfred and Wagner, Ernst
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2008): 309-20
Raman nanoparticle probes for antibody-based protein detection in tissues.
Authors: Lutz, Barry and Dentinger, Claire and Sun, Lei and Nguyen, Lienchi and Zhang, Jingwu and Chmura, Aj and Allen, April and Chan, Selena and Knudsen, Beatrice
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2008): 371-9
Rapid oxime and hydrazone ligations with aromatic aldehydes for biomolecular labeling.
Authors: Dirksen, Anouk and Dawson, Philip E
Journal: Bioconjugate chemistry (2008): 2543-8
Use of anti-fluorophore antibody to achieve high-sensitivity immunolocalizations of transporters and ion channels.
Authors: Coleman, Richard A and Liu, Jie and Wade, James B
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2006): 817-27
Oligonucleotide-arrayed TFT photosensor applicable for DNA chip technology.
Authors: Tanaka, Tsuyoshi and Hatakeyama, Keiichi and Sawaguchi, Masahiro and Iwadate, Akihito and Mizutani, Yasushi and Sasaki, Kazuhiro and Tateishi, Naofumi and Takeyama, Haruko and Matsunaga, Tadashi
Journal: Biotechnology and bioengineering (2006): 22-8
Acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils.
Authors: Amraei, Mohammad and Jia, Zongjian and Reboul, Pascal and Nabi, Ivan R
Journal: The Journal of biological chemistry (2003): 38935-41
Lipopolysaccharide (LPS) labeled with Alexa 488 hydrazide as a novel probe for LPS binding studies.
Authors: Triantafilou, K and Triantafilou, M and Fernandez, N
Journal: Cytometry (2000): 316-20