XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 717.68 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.30 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 71000 |
Excitation (nm) | 490 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Direct upgrades
iFluor® 488 Tetrazine |
Related products
Overview | ![]() ![]() |
Upgrade: iFluor® 488 Tetrazine
Molecular weight 717.68 | Correction Factor (260 nm) 0.30 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 71000 | Excitation (nm) 490 | Emission (nm) 520 | Quantum yield 0.921 |
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). XFD488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line. It is used for stable signal generation in imaging and flow cytometry. XFD488 tetrazine demonstrates exceptionally fast reaction with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any biorthogonal reaction. It provides a convenient tool for making biological conjugates with TCO-modified substrates. The resulted conjugates provide the bright, green fluorescence of XFD488.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 139.338 µL | 696.689 µL | 1.393 mL | 6.967 mL | 13.934 mL |
5 mM | 27.868 µL | 139.338 µL | 278.676 µL | 1.393 mL | 2.787 mL |
10 mM | 13.934 µL | 69.669 µL | 139.338 µL | 696.689 µL | 1.393 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.30 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 71000 |
Excitation (nm) | 490 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488* | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 TCO | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
Cy3 tetrazine | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
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Images
References
View all 37 references: Citation Explorer
Nanobody click chemistry for convenient site-specific fluorescent labelling, single step immunocytochemistry and delivery into living cells by photoporation and live cell imaging.
Authors: Hebbrecht, Tim and Liu, Jing and Zwaenepoel, Olivier and Boddin, Gaëlle and Van Leene, Chloé and Decoene, Klaas and Madder, Annemieke and Braeckmans, Kevin and Gettemans, Jan
Journal: New biotechnology (2020): 33-43
Authors: Hebbrecht, Tim and Liu, Jing and Zwaenepoel, Olivier and Boddin, Gaëlle and Van Leene, Chloé and Decoene, Klaas and Madder, Annemieke and Braeckmans, Kevin and Gettemans, Jan
Journal: New biotechnology (2020): 33-43
A novel tracer for in vivo optical imaging of fatty acid metabolism in the heart and brown adipose tissue.
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Journal: Scientific reports (2020): 11209
Authors: Panagia, Marcello and Yang, Jing and Gale, Eric and Wang, Huan and Luptak, Ivan and Chen, Howard H and Patel, Dakshesh and Croteau, Dominique and Pimentel, David Richard and Bachschmid, Markus Michael and Colucci, Wilson S and Ran, Chongzhao and Sosnovik, David E
Journal: Scientific reports (2020): 11209
Tylophorine reduces protein biosynthesis and rapidly decreases cyclin D1, inhibiting vascular smooth muscle cell proliferation in vitro and in organ culture.
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Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2019): 152938
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Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2019): 152938
PET imaging of EGFR expression using an 18F-labeled RNA aptamer.
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Journal: European journal of nuclear medicine and molecular imaging (2019): 948-956
Authors: Cheng, Siyuan and Jacobson, Orit and Zhu, Guizhi and Chen, Zhen and Liang, Steve H and Tian, Rui and Yang, Zhen and Niu, Gang and Zhu, Xiaohua and Chen, Xiaoyuan
Journal: European journal of nuclear medicine and molecular imaging (2019): 948-956
Long-term live cells observation of internalized fluorescent Fe@C nanoparticles in constant magnetic field.
Authors: Garanina, Anastasiia and Kireev, Igor and Zhironkina, Oxana and Strelkova, Olga and Shakhov, Anton and Alieva, Irina and Davydov, Valery and Murugesan, Sankaran and Khabashesku, Valery and Majouga, Alexander and Agafonov, Viatcheslav and Uzbekov, Rustem
Journal: Journal of nanobiotechnology (2019): 27
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Journal: Journal of nanobiotechnology (2019): 27
Cell-type-specific quantification of protein synthesis in vivo.
Authors: Hidalgo San Jose, Lorena and Signer, Robert A J
Journal: Nature protocols (2019): 441-460
Authors: Hidalgo San Jose, Lorena and Signer, Robert A J
Journal: Nature protocols (2019): 441-460
Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants.
Authors: Paper, Janet M and Mukherjee, Thiya and Schrick, Kathrin
Journal: Plant methods (2018): 31
Authors: Paper, Janet M and Mukherjee, Thiya and Schrick, Kathrin
Journal: Plant methods (2018): 31
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Authors: Martel, Makisha and Balleydier, Aurélien and Brochu, Julien and Drolet, Marc
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 131-138
Authors: Martel, Makisha and Balleydier, Aurélien and Brochu, Julien and Drolet, Marc
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 131-138
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Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2018): 829-836
Authors: Radicchio, Giovanna and Colicchia, Valeria and Marrapodi, Ramona and Carbonari, Maurizio
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2018): 829-836
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Journal: Nano research (2018): 464-476