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AAT Bioquest

XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*

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Physical properties
Molecular weight717.68
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.30
Correction Factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)71000
Excitation (nm)499
Emission (nm)520
Quantum yield0.921
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Direct upgrades
iFluor® 488 Tetrazine
Related products
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*
XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide*
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester*
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester*
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*
XFD350 C5 Maleimide *Same Structure to Alexa Fluor™ 350 C5 Maleimide*
XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide*
XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide*
XFD350 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350*
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*
XFD488 tyramide reagent *Same Structure to Alexa Fluor™ 488 tyramide*
XFD546 tyramide reagent *Same Structure to Alexa Fluor™ 546 tyramide*
XFD594 tyramide reagent *Same Structure to Alexa Fluor™ 594 tyramide*
XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488*
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester*
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester*
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*
XFD647 C2 Maleimide *Same Structure to Alexa Fluor™ 647 C2 Maleimide*
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*
XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*
XFD350 tyramide reagent *Same Structure to Alexa Fluor™ 350 tyramide*
XFD568 tyramide reagent *Same Structure to Alexa Fluor™ 568 tyramide*
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*
XFD™488-dUTP *1 mM in Tris Buffer (pH 7.5)*
XFD514 acid
XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester*
XFD514 tyramide
XFD532 tyramide
XFD647 Azide
XFD647 Alkyne
XFD532 amine
XFD555 amine
XFD568 amine
XFD647 amine
XFD750 amine
XFD555 acid
XFD647 acid
XFD750 acid
XFD700 acid
XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin*
XFD405 NHS Ester [equivalent to Alexa Fluor™ 405 NHS Ester]
Show More (46)

OverviewpdfSDSpdfProtocol


Molecular weight
717.68
Correction Factor (260 nm)
0.30
Correction Factor (280 nm)
0.11
Extinction coefficient (cm -1 M -1)
71000
Excitation (nm)
499
Emission (nm)
520
Quantum yield
0.921
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). XFD488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line. It is used for stable signal generation in imaging and flow cytometry. XFD488 tetrazine demonstrates exceptionally fast reaction with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any biorthogonal reaction. It provides a convenient tool for making biological conjugates with TCO-modified substrates. The resulted conjugates provide the bright, green fluorescence of XFD488.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM139.338 µL696.689 µL1.393 mL6.967 mL13.934 mL
5 mM27.868 µL139.338 µL278.676 µL1.393 mL2.787 mL
10 mM13.934 µL69.669 µL139.338 µL696.689 µL1.393 mL

Molarity calculator

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Spectrum


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Spectral properties

Correction Factor (260 nm)0.30
Correction Factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)71000
Excitation (nm)499
Emission (nm)520
Quantum yield0.921

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide*499520710000.9210.300.11
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne*499520710000.9210.300.11
XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine*499520710000.9210.300.11
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*499520710000.9210.300.11
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*499520730000.9210.30.11
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*499520710000.9210.300.11
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*499520710000.9210.300.11
XFD488 TCO499520730000.9210.30.11
Cy3 tetrazine55556915000010.1510.070.073
Cy5 tetrazine65167025000010.271, 0.420.020.03
Cy3B Tetrazine56057112000010.5810.0480.069
Cy7 tetrazine7567792500000.30.050.036
Show More (3)

Images


References


View all 37 references: Citation Explorer
Nanobody click chemistry for convenient site-specific fluorescent labelling, single step immunocytochemistry and delivery into living cells by photoporation and live cell imaging.
Authors: Hebbrecht, Tim and Liu, Jing and Zwaenepoel, Olivier and Boddin, Gaëlle and Van Leene, Chloé and Decoene, Klaas and Madder, Annemieke and Braeckmans, Kevin and Gettemans, Jan
Journal: New biotechnology (2020): 33-43
A novel tracer for in vivo optical imaging of fatty acid metabolism in the heart and brown adipose tissue.
Authors: Panagia, Marcello and Yang, Jing and Gale, Eric and Wang, Huan and Luptak, Ivan and Chen, Howard H and Patel, Dakshesh and Croteau, Dominique and Pimentel, David Richard and Bachschmid, Markus Michael and Colucci, Wilson S and Ran, Chongzhao and Sosnovik, David E
Journal: Scientific reports (2020): 11209
Tylophorine reduces protein biosynthesis and rapidly decreases cyclin D1, inhibiting vascular smooth muscle cell proliferation in vitro and in organ culture.
Authors: Joa, Helge and Blažević, Tina and Grojer, Christoph and Zeller, Iris and Heiss, Elke H and Atanasov, Atanas G and Feldler, Ines and Gruzdaitis, Päivi and Czaloun, Christa and Proksch, Peter and Messner, Barbara and Bernhard, David and Dirsch, Verena M
Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2019): 152938
PET imaging of EGFR expression using an 18F-labeled RNA aptamer.
Authors: Cheng, Siyuan and Jacobson, Orit and Zhu, Guizhi and Chen, Zhen and Liang, Steve H and Tian, Rui and Yang, Zhen and Niu, Gang and Zhu, Xiaohua and Chen, Xiaoyuan
Journal: European journal of nuclear medicine and molecular imaging (2019): 948-956
Long-term live cells observation of internalized fluorescent Fe@C nanoparticles in constant magnetic field.
Authors: Garanina, Anastasiia and Kireev, Igor and Zhironkina, Oxana and Strelkova, Olga and Shakhov, Anton and Alieva, Irina and Davydov, Valery and Murugesan, Sankaran and Khabashesku, Valery and Majouga, Alexander and Agafonov, Viatcheslav and Uzbekov, Rustem
Journal: Journal of nanobiotechnology (2019): 27
Cell-type-specific quantification of protein synthesis in vivo.
Authors: Hidalgo San Jose, Lorena and Signer, Robert A J
Journal: Nature protocols (2019): 441-460
Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants.
Authors: Paper, Janet M and Mukherjee, Thiya and Schrick, Kathrin
Journal: Plant methods (2018): 31
Detection of oriC-Independent Replication in Escherichia coli Cells.
Authors: Martel, Makisha and Balleydier, Aurélien and Brochu, Julien and Drolet, Marc
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 131-138
Flow cytometry in formamide treated cells.
Authors: Radicchio, Giovanna and Colicchia, Valeria and Marrapodi, Ramona and Carbonari, Maurizio
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2018): 829-836
Intracellular in situ labeling of TiO2 nanoparticles for fluorescence microscopy detection.
Authors: Brown, Koshonna and Thurn, Ted and Xin, Lun and Liu, William and Bazak, Remon and Chen, Si and Lai, Barry and Vogt, Stefan and Jacobsen, Chris and Paunesku, Tatjana and Woloschak, Gayle E
Journal: Nano research (2018): 464-476