XFD488 tetrazine Same Structure to Alexa Fluor™ 488 tetrazine

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	717.68
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.30
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	490
Emission (nm)	520
Quantum yield	0.92¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Direct upgrades

iFluor® 488 Tetrazine

Overview SDS Protocol

Upgrade: iFluor® 488 Tetrazine

Molecular weight

717.68

Correction Factor (260 nm)

0.30

Correction Factor (280 nm)

0.11

Extinction coefficient (cm ^-1 M ^-1)

71000

Excitation (nm)

490

Emission (nm)

520

Quantum yield

0.92¹

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). XFD488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line. It is used for stable signal generation in imaging and flow cytometry. XFD488 tetrazine demonstrates exceptionally fast reaction with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any biorthogonal reaction. It provides a convenient tool for making biological conjugates with TCO-modified substrates. The resulted conjugates provide the bright, green fluorescence of XFD488.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	139.338 µL	696.689 µL	1.393 mL	6.967 mL	13.934 mL
5 mM	27.868 µL	139.338 µL	278.676 µL	1.393 mL	2.787 mL
10 mM	13.934 µL	69.669 µL	139.338 µL	696.689 µL	1.393 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.30
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	490
Emission (nm)	520
Quantum yield	0.92¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 azide Same Structure to Alexa Fluor™ 488 azide	490	520	71000	0.92¹	0.30	0.11
XFD488 alkyne Same Structure to Alexa Fluor™ 488 alkyne	490	520	71000	0.92¹	0.30	0.11
XFD488 Hydroxylamine Same Structure to Alexa Fluor™ 488 Hydroxylamine	490	520	71000	0.92¹	0.30	0.11
XFD488 amine Same Structure to Alexa Fluor™ 488 amine	490	520	71000	0.92¹	0.30	0.11
XFD488 Phalloidin XFD488 Same Structure to Alexa Fluor™ 488	499	520	73000	0.92¹	0.3	0.11
XFD488 acid Same Structure to Alexa Fluor™ 488 acid	490	520	71000	0.92¹	0.30	0.11
XFD488 aldehyde Same Structure to Alexa Fluor™ 488 aldehyde	490	520	71000	0.92¹	0.30	0.11
XFD488 TCO	499	520	73000	0.92¹	0.3	0.11
Cy3 tetrazine	555	569	150000¹	0.15¹	0.07	0.073
Cy5 tetrazine	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy3B Tetrazine	560	571	120000¹	0.58¹	0.048	0.069
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Images

Figure 1. Chemical structure for XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*.

References

View all 37 references: Citation Explorer

Nanobody click chemistry for convenient site-specific fluorescent labelling, single step immunocytochemistry and delivery into living cells by photoporation and live cell imaging.
Authors: Hebbrecht, Tim and Liu, Jing and Zwaenepoel, Olivier and Boddin, Gaëlle and Van Leene, Chloé and Decoene, Klaas and Madder, Annemieke and Braeckmans, Kevin and Gettemans, Jan
Journal: New biotechnology (2020): 33-43

A novel tracer for in vivo optical imaging of fatty acid metabolism in the heart and brown adipose tissue.
Authors: Panagia, Marcello and Yang, Jing and Gale, Eric and Wang, Huan and Luptak, Ivan and Chen, Howard H and Patel, Dakshesh and Croteau, Dominique and Pimentel, David Richard and Bachschmid, Markus Michael and Colucci, Wilson S and Ran, Chongzhao and Sosnovik, David E
Journal: Scientific reports (2020): 11209

Tylophorine reduces protein biosynthesis and rapidly decreases cyclin D1, inhibiting vascular smooth muscle cell proliferation in vitro and in organ culture.
Authors: Joa, Helge and Blažević, Tina and Grojer, Christoph and Zeller, Iris and Heiss, Elke H and Atanasov, Atanas G and Feldler, Ines and Gruzdaitis, Päivi and Czaloun, Christa and Proksch, Peter and Messner, Barbara and Bernhard, David and Dirsch, Verena M
Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2019): 152938

PET imaging of EGFR expression using an 18F-labeled RNA aptamer.
Authors: Cheng, Siyuan and Jacobson, Orit and Zhu, Guizhi and Chen, Zhen and Liang, Steve H and Tian, Rui and Yang, Zhen and Niu, Gang and Zhu, Xiaohua and Chen, Xiaoyuan
Journal: European journal of nuclear medicine and molecular imaging (2019): 948-956

Long-term live cells observation of internalized fluorescent Fe@C nanoparticles in constant magnetic field.
Authors: Garanina, Anastasiia and Kireev, Igor and Zhironkina, Oxana and Strelkova, Olga and Shakhov, Anton and Alieva, Irina and Davydov, Valery and Murugesan, Sankaran and Khabashesku, Valery and Majouga, Alexander and Agafonov, Viatcheslav and Uzbekov, Rustem
Journal: Journal of nanobiotechnology (2019): 27

Cell-type-specific quantification of protein synthesis in vivo.
Authors: Hidalgo San Jose, Lorena and Signer, Robert A J
Journal: Nature protocols (2019): 441-460

Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants.
Authors: Paper, Janet M and Mukherjee, Thiya and Schrick, Kathrin
Journal: Plant methods (2018): 31

Detection of oriC-Independent Replication in Escherichia coli Cells.
Authors: Martel, Makisha and Balleydier, Aurélien and Brochu, Julien and Drolet, Marc
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 131-138

Flow cytometry in formamide treated cells.
Authors: Radicchio, Giovanna and Colicchia, Valeria and Marrapodi, Ramona and Carbonari, Maurizio
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2018): 829-836

Intracellular in situ labeling of TiO2 nanoparticles for fluorescence microscopy detection.
Authors: Brown, Koshonna and Thurn, Ted and Xin, Lun and Liu, William and Bazak, Remon and Chen, Si and Lai, Barry and Vogt, Stefan and Jacobsen, Chris and Paunesku, Tatjana and Woloschak, Gayle E
Journal: Nano research (2018): 464-476