XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*
| Overview |  SDSProtocol |
Upgrade: iFluor® 488 amine
Molecular weight 805.61 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 499 | Emission (nm) 520 | Quantum yield 0.921 |
XFD488 amine is the same molecule to Alexa Fluor® 488 C5 amine. The fluorescent dye amines are reactive molecules that can be used to add a fluorescent label to biomolecules containing a carbonyl group. XFD488 amine reacts with carboxylic acids, aldehydes and ketones. It is a very useful building block for developing XFD488 probes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 124.13 µL | 620.648 µL | 1.241 mL | 6.206 mL | 12.413 mL |
| 5 mM | 24.826 µL | 124.13 µL | 248.259 µL | 1.241 mL | 2.483 mL |
| 10 mM | 12.413 µL | 62.065 µL | 124.13 µL | 620.648 µL | 1.241 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
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Spectral properties
| Extinction coefficient (cm -1 M -1) | 73000 |
| Excitation (nm) | 499 |
| Emission (nm) | 520 |
| Quantum yield | 0.921 |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine* | 499 | 520 | 73000 | 0.921 |
| XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 590 | 618 | 92000 | 0.661 |
| XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488* | 499 | 520 | 73000 | 0.921 |
| XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* | 499 | 520 | 73000 | 0.921 |
| XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* | 499 | 520 | 73000 | 0.921 |
| XFD488 TCO | 499 | 520 | 73000 | 0.921 |
| ICG amine | 789 | 814 | - | - |
| DNP amine | - | - | - | - |
| Phalloidin Amine | - | - | - | - |
References
View all 39 references: Citation Explorer
Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
A mouse monoclonal antibody against Alexa Fluor 647
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20
Volume labeling with Alexa Fluor dyes and surface functionalization of highly sensitive fluorescent silica (SiO2) nanoparticles
Authors: Wang, W.; Nallathamby, P. D.; Foster, C. M.; Morrell-Falvey, J. L.; Mortensen, N. P.; Doktycz, M. J.; Gu, B.; Retterer, S. T.
Journal: Nanoscale (2013): 10369-75
Authors: Wang, W.; Nallathamby, P. D.; Foster, C. M.; Morrell-Falvey, J. L.; Mortensen, N. P.; Doktycz, M. J.; Gu, B.; Retterer, S. T.
Journal: Nanoscale (2013): 10369-75
Green tea catechins quench the fluorescence of bacteria-conjugated Alexa fluor dyes
Authors: Zhao, L.; Li, W.; Zhu, S.; Tsai, S.; Li, J.; Tracey, K. J.; Wang, P.; Fan, S.; Sama, A. E.; Wang, H.
Journal: Inflamm Allergy Drug Targets (2013): 308-14
Authors: Zhao, L.; Li, W.; Zhu, S.; Tsai, S.; Li, J.; Tracey, K. J.; Wang, P.; Fan, S.; Sama, A. E.; Wang, H.
Journal: Inflamm Allergy Drug Targets (2013): 308-14
Fluorescence of Alexa fluor dye tracks protein folding
Authors: Lindhoud, S.; Westphal, A. H.; Visser, A. J.; Borst, J. W.; van Mierlo, C. P.
Journal: PLoS One (2012): e46838
Authors: Lindhoud, S.; Westphal, A. H.; Visser, A. J.; Borst, J. W.; van Mierlo, C. P.
Journal: PLoS One (2012): e46838
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)