XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 805.61 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.30 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 71000 |
Excitation (nm) | 490 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Direct upgrades
iFluor® 488 amine |
Alternative formats
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid* |
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide* |
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* |
Related products
Overview | ![]() ![]() |
Upgrade: iFluor® 488 amine
Molecular weight 805.61 | Correction Factor (260 nm) 0.30 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 71000 | Excitation (nm) 490 | Emission (nm) 520 | Quantum yield 0.921 |
XFD488 amine is the same molecule to Alexa Fluor® 488 C5 amine. The fluorescent dye amines are reactive molecules that can be used to add a fluorescent label to biomolecules containing a carbonyl group. XFD488 amine reacts with carboxylic acids, aldehydes and ketones. It is a very useful building block for developing XFD488 probes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 124.13 µL | 620.648 µL | 1.241 mL | 6.206 mL | 12.413 mL |
5 mM | 24.826 µL | 124.13 µL | 248.259 µL | 1.241 mL | 2.483 mL |
10 mM | 12.413 µL | 62.065 µL | 124.13 µL | 620.648 µL | 1.241 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.30 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 71000 |
Excitation (nm) | 490 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 375 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488* | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD488 TCO | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
XFD532 amine | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
XFD555 amine | 553 | 568 | 155000 | 0.11 | 0.08 | 0.08 |
XFD568 amine | 579 | 603 | 88000 | 0.691 | 0.45 | 0.46 |
Show More (3) |
References
View all 39 references: Citation Explorer
Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
A mouse monoclonal antibody against Alexa Fluor 647
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20
Volume labeling with Alexa Fluor dyes and surface functionalization of highly sensitive fluorescent silica (SiO2) nanoparticles
Authors: Wang, W.; Nallathamby, P. D.; Foster, C. M.; Morrell-Falvey, J. L.; Mortensen, N. P.; Doktycz, M. J.; Gu, B.; Retterer, S. T.
Journal: Nanoscale (2013): 10369-75
Authors: Wang, W.; Nallathamby, P. D.; Foster, C. M.; Morrell-Falvey, J. L.; Mortensen, N. P.; Doktycz, M. J.; Gu, B.; Retterer, S. T.
Journal: Nanoscale (2013): 10369-75
Green tea catechins quench the fluorescence of bacteria-conjugated Alexa fluor dyes
Authors: Zhao, L.; Li, W.; Zhu, S.; Tsai, S.; Li, J.; Tracey, K. J.; Wang, P.; Fan, S.; Sama, A. E.; Wang, H.
Journal: Inflamm Allergy Drug Targets (2013): 308-14
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Journal: Inflamm Allergy Drug Targets (2013): 308-14
Fluorescence of Alexa fluor dye tracks protein folding
Authors: Lindhoud, S.; Westphal, A. H.; Visser, A. J.; Borst, J. W.; van Mierlo, C. P.
Journal: PLoS One (2012): e46838
Authors: Lindhoud, S.; Westphal, A. H.; Visser, A. J.; Borst, J. W.; van Mierlo, C. P.
Journal: PLoS One (2012): e46838
Visualizing dengue virus through Alexa Fluor labeling
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Journal: J Vis Exp. (2011)
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Journal: J Vis Exp. (2011)