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AAT Bioquest

XFD660 amine

Product key features

  • Ex/Em: 663/691 nm
  • Extinction coefficient: 132,000 cm-1M-1
  • Superior Fluorescence Performance: High quantum yield, exceptional photostability, and pH-independent fluorescence across pH 4–11.
  • Reactive Group: Amine
  • Easy Conjugation: Efficiently tags carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
  • High Water-Solubility: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Product description

XFD660, manufactured by AAT Bioquest, is a far-red fluorescent dye structurally equivalent to Alexa Fluor™ 660 (ThermoFisher). The dye demonstrates high fluorescence quantum yield, photostability, and aqueous solubility, with pH-independent fluorescence across a broad range (pH 4–11), providing consistent performance across diverse experimental conditions.

XFD660 is optimized for red laser excitation and is compatible with flow cytometers equipped with spectral detection systems. It provides robust and uniform labeling with high signal intensity and reproducibility, making it ideal for fluorescence imaging, flow cytometry, and other analytical techniques. XFD660 demonstrates versatility in labeling a wide range of targets, including cell surface, intracellular, and intranuclear antigens. Its spectral properties position it between XFD647 and XFD700, making it a valuable intermediate fluorophore for constructing complex multicolor panels and enabling precise experimental designs in advanced research workflows.

XFD660 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD350 amine34344119000-0.250.19
XFD405 amine40142135,000-0.230.70
XFD430 amine43254015,000--0.28
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*499520710000.9210.300.11
XFD514 amine51854380000-0.310.18
XFD532 amine534553810000.6110.240.09
XFD546 amine5615721120000.7910.210.12
XFD555 amine5535681550000.110.080.08
XFD568 amine579603913000.6910.450.46
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.6610.430.56
XFD610 amine611629144,000-0.430.44
XFD633 amine632650159,000--0.55
XFD635 amine633647140,000---
XFD647 amine6506712700000.331-0.03
XFD660 TCO663691132000-0.000.10
XFD660 Tetrazine663691132000-0.000.10
XFD680 amine6817041840000.3610.000.05
XFD700 amine6967191920000.2510.000.07
XFD750 amine7527762900000.121-0.04
XFD790 amine782805260,000-0.090.08
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Page updated on May 19, 2025

Ordering information

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Unit size
Catalog Number70093
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
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Physical properties

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.00

Correction Factor (280 nm)

0.10

Extinction coefficient (cm -1 M -1)

132000

Excitation (nm)

663

Emission (nm)

691

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Schematic illustrating the carboxyl–amine coupling between an amine‐functionalized dye and a carboxyl‐modified biomolecule. In practice, carbodiimide reagents (e.g., EDC) are often used to activate carboxyl groups, facilitating subsequent amide bond formation with primary amines. This versatile approach enables conjugation of peptides, proteins, and nucleic acids that possess free carboxyl or amine functionalities.
Schematic illustrating the carboxyl–amine coupling between an amine‐functionalized dye and a carboxyl‐modified biomolecule. In practice, carbodiimide reagents (e.g., EDC) are often used to activate carboxyl groups, facilitating subsequent amide bond formation with primary amines. This versatile approach enables conjugation of peptides, proteins, and nucleic acids that possess free carboxyl or amine functionalities.
Schematic illustrating the carboxyl–amine coupling between an amine‐functionalized dye and a carboxyl‐modified biomolecule. In practice, carbodiimide reagents (e.g., EDC) are often used to activate carboxyl groups, facilitating subsequent amide bond formation with primary amines. This versatile approach enables conjugation of peptides, proteins, and nucleic acids that possess free carboxyl or amine functionalities.