XFD568 amine
Ordering information
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 850.83 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.45 |
| Correction Factor (280 nm) | 0.46 |
| Extinction coefficient (cm -1 M -1) | 88000 |
| Excitation (nm) | 579 |
| Emission (nm) | 603 |
| Quantum yield | 0.691 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
| Overview |  SDSProtocol |
Molecular weight 850.83 | Correction Factor (260 nm) 0.45 | Correction Factor (280 nm) 0.46 | Extinction coefficient (cm -1 M -1) 88000 | Excitation (nm) 579 | Emission (nm) 603 | Quantum yield 0.691 |
XFD568 amine is the same molecule to the Alexa Fluor® 568 amine (Alexa Fluor® is the trademark of ThermoFisher). It is a rhodamine dye with bright red-fluorescence optimal for labeling antibodies. XFD568 dye is water soluble and pH-insensitive from pH 4 to 10. The amine of XFD568 is a versatile carbonyl-reactive form for conjugating this fluorophore to peptides and other biological molecules.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD568 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 117.532 µL | 587.661 µL | 1.175 mL | 5.877 mL | 11.753 mL |
| 5 mM | 23.506 µL | 117.532 µL | 235.065 µL | 1.175 mL | 2.351 mL |
| 10 mM | 11.753 µL | 58.766 µL | 117.532 µL | 587.661 µL | 1.175 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.45 |
| Correction Factor (280 nm) | 0.46 |
| Extinction coefficient (cm -1 M -1) | 88000 |
| Excitation (nm) | 579 |
| Emission (nm) | 603 |
| Quantum yield | 0.691 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 590 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
| XFD568 acid *Same Structure to Alexa Fluor™ 568 acid* | 579 | 603 | 91300 | 0.691 | 0.45 | 0.46 |
| XFD532 amine | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD555 amine | 553 | 568 | 155000 | 0.11 | 0.08 | 0.08 |
| XFD647 amine | 650 | 671 | 270000 | 0.331 | - | 0.03 |
| XFD750 amine | 752 | 776 | 290000 | 0.121 | - | 0.04 |
| ICG amine | 789 | 814 | - | - | - | 0.076 |
Images
Figure 1. XFD568 amine is the same molecule to the Alexa Fluor® 568 amine. It is a rhodamine dye with bright red-fluorescence optimal for labeling antibodies.
References
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Enhancing interaction of actin and actin-binding domain 1 of dystrophin with modulators: Toward improved gene therapy for Duchenne muscular dystrophy.
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Journal: The Journal of biological chemistry (2022): 102675
Authors: Guhathakurta, Piyali and Carter, Anna L and Thompson, Andrew R and Kurila, Dillon and LaFrence, Jeffrey and Zhang, Li and Trask, Jake R and Vanderheyden, Bri and Muretta, Joseph M and Ervasti, James M and Thomas, David D
Journal: The Journal of biological chemistry (2022): 102675
Detection and quantification of the vacuolar H+ATPase using the Legionella effector protein SidK.
Authors: Maxson, Michelle E and Abbas, Yazan M and Wu, Jing Ze and Plumb, Jonathan D and Grinstein, Sergio and Rubinstein, John L
Journal: The Journal of cell biology (2022)
Authors: Maxson, Michelle E and Abbas, Yazan M and Wu, Jing Ze and Plumb, Jonathan D and Grinstein, Sergio and Rubinstein, John L
Journal: The Journal of cell biology (2022)
A naked antisense oligonucleotide with phosphorothioate linkages is taken up intracellularly more efficiently but functions less effectively.
Authors: Takahashi, Masayuki and Seki, Mineaki and Nashimoto, Masayuki
Journal: Biochemical and biophysical research communications (2021): 140-144
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Journal: Biochemical and biophysical research communications (2021): 140-144
Dual-Labeled Graphene Quantum Dot-Based Förster Resonance Energy Transfer Nanoprobes for Single-Molecule Localization Microscopy.
Authors: Lu, Ju and Zong, Shenfei and Wang, Zhuyuan and Chen, Chen and Zhang, Yizhi and Wang, Hong and Cui, Yiping
Journal: ACS omega (2021): 8808-8815
Authors: Lu, Ju and Zong, Shenfei and Wang, Zhuyuan and Chen, Chen and Zhang, Yizhi and Wang, Hong and Cui, Yiping
Journal: ACS omega (2021): 8808-8815
Cardiac myosin-binding protein C interaction with actin is inhibited by compounds identified in a high-throughput fluorescence lifetime screen.
Authors: Bunch, Thomas A and Guhathakurta, Piyali and Lepak, Victoria C and Thompson, Andrew R and Kanassatega, Rhye-Samuel and Wilson, Anna and Thomas, David D and Colson, Brett A
Journal: The Journal of biological chemistry (2021): 100840
Authors: Bunch, Thomas A and Guhathakurta, Piyali and Lepak, Victoria C and Thompson, Andrew R and Kanassatega, Rhye-Samuel and Wilson, Anna and Thomas, David D and Colson, Brett A
Journal: The Journal of biological chemistry (2021): 100840
Switching behaviour of dSTORM dyes in glycerol-containing buffer.
Authors: Goossen-Schmidt, Nora C and Schnieder, Marco and Hüve, Jana and Klingauf, Jürgen
Journal: Scientific reports (2020): 13746
Authors: Goossen-Schmidt, Nora C and Schnieder, Marco and Hüve, Jana and Klingauf, Jürgen
Journal: Scientific reports (2020): 13746
Effect of amisulpride, olanzapine, quetiapine, and aripiprazole single administration on c-Fos expression in vasopressinergic and oxytocinergic neurons of the rat hypothalamic supraoptic nucleus.
Authors: Kiss, Alexander and Osacka, Jana
Journal: Endocrine regulations (2020): 77-84
Authors: Kiss, Alexander and Osacka, Jana
Journal: Endocrine regulations (2020): 77-84
Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568.
Authors: Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós
Journal: Journal of fluorescence (2020): 437-443
Authors: Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós
Journal: Journal of fluorescence (2020): 437-443
Suppression of Connexin 43 Leads to Strial Vascular Hyper-Permeability, Decrease in Endocochlear Potential, and Mild Hearing Loss.
Authors: Zhang, Jinhui and Wang, Xiaohan and Hou, Zhiqiang and Neng, Lingling and Cai, Jing and Zhang, Yunpei and Shi, Xiaorui
Journal: Frontiers in physiology (2020): 974
Authors: Zhang, Jinhui and Wang, Xiaohan and Hou, Zhiqiang and Neng, Lingling and Cai, Jing and Zhang, Yunpei and Shi, Xiaorui
Journal: Frontiers in physiology (2020): 974
Phosphatidylserine Asymmetry Promotes the Membrane Insertion of a Transmembrane Helix.
Authors: Scott, Haden L and Heberle, Frederick A and Katsaras, John and Barrera, Francisco N
Journal: Biophysical journal (2019): 1495-1506
Authors: Scott, Haden L and Heberle, Frederick A and Katsaras, John and Barrera, Francisco N
Journal: Biophysical journal (2019): 1495-1506