XFD568 amine

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Physical properties

Molecular weight	850.83
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.45
Correction Factor (280 nm)	0.46
Extinction coefficient (cm ^-1 M ^-1)	88000
Excitation (nm)	579
Emission (nm)	603
Quantum yield	0.69¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD568 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	117.532 µL	587.661 µL	1.175 mL	5.877 mL	11.753 mL
5 mM	23.506 µL	117.532 µL	235.065 µL	1.175 mL	2.351 mL
10 mM	11.753 µL	58.766 µL	117.532 µL	587.661 µL	1.175 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.45
Correction Factor (280 nm)	0.46
Extinction coefficient (cm ^-1 M ^-1)	88000
Excitation (nm)	579
Emission (nm)	603
Quantum yield	0.69¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
XFD488 amine Same Structure to Alexa Fluor™ 488 amine	499	520	71000	0.92¹	0.11
XFD594 amine Same Structure to Alexa Fluor™ 594 amine	590	618	90000	0.66¹	0.56
XFD568 acid Same Structure to Alexa Fluor™ 568 acid	579	603	91300	0.69¹	0.46
XFD532 amine	534	553	81000	0.61¹	0.09
XFD555 amine	553	568	155000	0.1¹	0.08
XFD647 amine	650	671	270000	0.33¹	0.03
XFD750 amine	752	776	290000	0.12¹	0.04
ICG amine	789	813	230000	-	0.076

Images

Figure 1. XFD568 amine is the same molecule to the Alexa Fluor® 568 amine. It is a rhodamine dye with bright red-fluorescence optimal for labeling antibodies.

References

View all 50 references: Citation Explorer

Enhancing interaction of actin and actin-binding domain 1 of dystrophin with modulators: Toward improved gene therapy for Duchenne muscular dystrophy.
Authors: Guhathakurta, Piyali and Carter, Anna L and Thompson, Andrew R and Kurila, Dillon and LaFrence, Jeffrey and Zhang, Li and Trask, Jake R and Vanderheyden, Bri and Muretta, Joseph M and Ervasti, James M and Thomas, David D
Journal: The Journal of biological chemistry (2022): 102675

Detection and quantification of the vacuolar H+ATPase using the Legionella effector protein SidK.
Authors: Maxson, Michelle E and Abbas, Yazan M and Wu, Jing Ze and Plumb, Jonathan D and Grinstein, Sergio and Rubinstein, John L
Journal: The Journal of cell biology (2022)

A naked antisense oligonucleotide with phosphorothioate linkages is taken up intracellularly more efficiently but functions less effectively.
Authors: Takahashi, Masayuki and Seki, Mineaki and Nashimoto, Masayuki
Journal: Biochemical and biophysical research communications (2021): 140-144

Dual-Labeled Graphene Quantum Dot-Based Förster Resonance Energy Transfer Nanoprobes for Single-Molecule Localization Microscopy.
Authors: Lu, Ju and Zong, Shenfei and Wang, Zhuyuan and Chen, Chen and Zhang, Yizhi and Wang, Hong and Cui, Yiping
Journal: ACS omega (2021): 8808-8815

Cardiac myosin-binding protein C interaction with actin is inhibited by compounds identified in a high-throughput fluorescence lifetime screen.
Authors: Bunch, Thomas A and Guhathakurta, Piyali and Lepak, Victoria C and Thompson, Andrew R and Kanassatega, Rhye-Samuel and Wilson, Anna and Thomas, David D and Colson, Brett A
Journal: The Journal of biological chemistry (2021): 100840

Switching behaviour of dSTORM dyes in glycerol-containing buffer.
Authors: Goossen-Schmidt, Nora C and Schnieder, Marco and Hüve, Jana and Klingauf, Jürgen
Journal: Scientific reports (2020): 13746

Effect of amisulpride, olanzapine, quetiapine, and aripiprazole single administration on c-Fos expression in vasopressinergic and oxytocinergic neurons of the rat hypothalamic supraoptic nucleus.
Authors: Kiss, Alexander and Osacka, Jana
Journal: Endocrine regulations (2020): 77-84

Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568.
Authors: Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós
Journal: Journal of fluorescence (2020): 437-443

Suppression of Connexin 43 Leads to Strial Vascular Hyper-Permeability, Decrease in Endocochlear Potential, and Mild Hearing Loss.
Authors: Zhang, Jinhui and Wang, Xiaohan and Hou, Zhiqiang and Neng, Lingling and Cai, Jing and Zhang, Yunpei and Shi, Xiaorui
Journal: Frontiers in physiology (2020): 974

Phosphatidylserine Asymmetry Promotes the Membrane Insertion of a Transmembrane Helix.
Authors: Scott, Haden L and Heberle, Frederick A and Katsaras, John and Barrera, Francisco N
Journal: Biophysical journal (2019): 1495-1506