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AAT Bioquest

XFD532 Tetrazine

Product key features

  • Ex/Em: 534/553 nm
  • Extinction coefficient: 81,000 cm-1M-1
  • Reactive Group: Tetrazine
  • Click Chemistry Conjugation: Provides rapid, copper-free labeling of TCO-modified biomolecules
  • Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
  • Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell studies

Product description

XFD532, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 532 (ThermoFisher). It is a bright yellow-fluorescent dye with an excitation optimized for use with the 532 nm line of the frequency-doubled Nd:YAG laser. XFD532 demonstrates good aqueous solubility and pH-insensitivity over a broad pH range (pH 4–10), ensuring stable fluorescence generation under varying experimental conditions. This dye is particularly suited for multicolor fluorescence microscopy and flow cytometry, as well as advanced applications in super-resolution imaging techniques such as dSTORM.

XFD532 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

References

View all 50 references: Citation Explorer
Multiplexed DNA and Protease Detection with Orthogonal Energy Transfer on a Single Quantum Dot Scaffolded Biosensor.
Authors: Hastman, David A and Hooe, Shelby and Chiriboga, Matthew and Díaz, Sebastián A and Susumu, Kimihiro and Stewart, Michael H and Green, Christopher M and Hildebrandt, Niko and Medintz, Igor L
Journal: ACS sensors (2024): 157-170
Proximity Estimation and Quantification of Ionizing Radiation-induced DNA Lesions in Aqueous Media using Fluorescence Spectroscopy.
Authors: Akamatsu, Ken and Satoh, Katsuya and Shikazono, Naoya and Saito, Takeshi
Journal: Radiation research (2023)
Possible frequent multiple mitochondrial DNA copies in a single nucleoid in HeLa cells.
Authors: Pavluch, Vojtěch and Špaček, Tomáš and Engstová, Hana and Dlasková, Andrea and Ježek, Petr
Journal: Scientific reports (2023): 5788
Exclusion of Anchor-Matched Peptide Nucleic Acid from Liquid-Ordered Domains by Hybridization with Complementary Flavin-Labeled DNA.
Authors: Oka, Yoshimi
Journal: ACS omega (2023): 1109-1113
1,4-Naphthoquinone (CNN1) Induces Apoptosis through DNA Damage and Promotes Upregulation of H2AFX in Leukemia Multidrug Resistant Cell Line.
Authors: de Sousa Portilho, Adrhyann Jullyanne and da Silva, Emerson Lucena and Bezerra, Emanuel Cintra Austregésilo and Moraes Rego Gomes, Carinne Borges de Souza and Ferreira, Vitor and de Moraes, Maria Elisabete Amaral and da Rocha, David Rodrigues and Burbano, Rommel Mário Rodriguez and Moreira-Nunes, Caroline Aquino and Montenegro, Raquel Carvalho
Journal: International journal of molecular sciences (2022)
Page updated on April 25, 2025

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Catalog Number1721
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Physical properties

Molecular weight

911.11

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.09

Extinction coefficient (cm -1 M -1)

81000

Excitation (nm)

534

Emission (nm)

553

Quantum yield

0.611

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Product Image
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Gallery Image 1
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a tetrazine–dye conjugate and a trans‐cyclooctene (TCO)–modified biomolecule. The tetrazine–dye selectively and rapidly reacts with the TCO moiety on the biomolecule to form a stable linkage, labeling the nucleic acid with the dye. This bioorthogonal “click chemistry” process is fast, selective, and well‐suited for biocompatible labeling applications.