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AAT Bioquest

XFD532 alkyne

Product key features

  • Ex/Em: 534/553 nm
  • Extinction coefficient: 81,000 cm-1M-1
  • Reactive Group: Alkyne
  • Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
  • Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
  • Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell studies

Product description

XFD532, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 532 (ThermoFisher). It is a bright yellow-fluorescent dye with an excitation optimized for use with the 532 nm line of the frequency-doubled Nd:YAG laser. XFD532 demonstrates good aqueous solubility and pH-insensitivity over a broad pH range (pH 4–10), ensuring stable fluorescence generation under varying experimental conditions. This dye is particularly suited for multicolor fluorescence microscopy and flow cytometry, as well as advanced applications in super-resolution imaging techniques such as dSTORM.

The alkyne derivative of XFD532 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.

Spectrum

References

View all 47 references: Citation Explorer
Cell-Penetrating and Enzyme-Responsive Peptides for Targeted Cancer Therapy: Role of Arginine Residue Length on Cell Penetration and In Vivo Systemic Toxicity.
Authors: Ghaemi, Behnaz and Tanwar, Swati and Singh, Aruna and Arifin, Dian R and McMahon, Michael T and Barman, Ishan and Bulte, Jeff W M
Journal: ACS applied materials & interfaces (2024): 11159-11171
Click-Functionalization of Silanized Carbon Nanotubes: From Inorganic Heterostructures to Biosensing Nanohybrids.
Authors: Manoharan, Gririraj and Bösel, Petra and Thien, Jannis and Holtmannspötter, Michael and Meingast, Laura and Schmidt, Mercedes and Eickmeier, Henning and Haase, Markus and Maultzsch, Janina and Steinhart, Martin and Wollschläger, Joachim and Palma, Matteo and Meyer, Carola
Journal: Molecules (Basel, Switzerland) (2023)
Click-iT ® Plus OPP Alexa Fluor ® Protein Synthesis Assay in Embryonic Cells.
Authors: Li, Yan and Ji, Xu and Chang, Lu and Tang, Jianan and Hua, Min-Min and Liu, Jing and O'Neill, Christopher and Huang, Xuefeng and Jin, Xingliang
Journal: Bio-protocol (2022): e4441
Recyclable cell-surface chemical tags for repetitive cancer targeting.
Authors: Bhatta, Rimsha and Han, Joonsu and Zhou, Jingyi and Li, Haoyu and Wang, Hua
Journal: Journal of controlled release : official journal of the Controlled Release Society (2022): 164-174
Protocol for visualizing newly synthesized proteins in primary mouse hepatocytes.
Authors: Shen, Yuqian and Liu, Wenhua and Zuo, Jian and Han, Junhai and Zhang, Zi Chao
Journal: STAR protocols (2021): 100616
Page updated on April 25, 2025

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Catalog Number1718
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Physical properties

Molecular weight

764.96

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.09

Extinction coefficient (cm -1 M -1)

81000

Excitation (nm)

534

Emission (nm)

553

Quantum yield

0.611

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Product Image
Product Image
Gallery Image 1
Schematic illustrating the azide–alkyne cycloaddition (“click chemistry”) between an alkyne‐functionalized dye and an azide‐presenting biomolecule. In the presence of a copper catalyst (CuAAC) or under strain‐promoted conditions (SPAAC), the azide and alkyne react to form a stable 1,2,3‐triazole linkage. This highly selective and robust reaction proceeds under mild conditions, tolerates a wide range of functional groups, and is frequently used to label proteins, nucleic acids, and other biomolecules for imaging, proteomics, and high‐throughput assays.