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AAT Bioquest

XFD532 TCO

Product key features

  • Ex/Em: 534/553 nm
  • Extinction coefficient: 81,000 cm-1M-1
  • Reactive Group: TCO
  • Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules
  • Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
  • Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell studies

Product description

XFD532, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 532 (ThermoFisher). It is a bright yellow-fluorescent dye with an excitation optimized for use with the 532 nm line of the frequency-doubled Nd:YAG laser. XFD532 demonstrates good aqueous solubility and pH-insensitivity over a broad pH range (pH 4–10), ensuring stable fluorescence generation under varying experimental conditions. This dye is particularly suited for multicolor fluorescence microscopy and flow cytometry, as well as advanced applications in super-resolution imaging techniques such as dSTORM.

XFD532 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

References

View all 18 references: Citation Explorer
Spectroscopic and solution properties characterization of quaternary ammonium ion containing polycations complexed with fluorescent rhodamine sulfonic acid dyes.
Authors: Johnson, Anna M and Hamilton, Charles and Jutton, Frederick and Carter, Phillip W and Remsen, Edward E
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2025): 124996
Measurement of intracellular concentration of fluorescently-labeled targets in living cells.
Authors: Cherkas, Volodymyr and Grebenyuk, Sergei and Osypenko, Denys and Dovgan, Alexandr V and Grushevskyi, Eugene O and Yedutenko, Matthew and Sheremet, Yevhenii and Dromaretsky, Andrew and Bozhenko, Arseniy and Agashkov, Kirill and Kononenko, Nikolai I and Belan, Pavel
Journal: PloS one (2018): e0194031
Enhanced Release of Molecules upon Ultraviolet (UV) Light Irradiation from Photoresponsive Hydrogels Prepared from Bifunctional Azobenzene and Four-Arm Poly(ethylene glycol).
Authors: Rastogi, Shiva K and Anderson, Hailee E and Lamas, Joseph and Barret, Scott and Cantu, Travis and Zauscher, Stefan and Brittain, William J and Betancourt, Tania
Journal: ACS applied materials & interfaces (2018): 30071-30080
Cerebrospinal fluid injection into adult zebrafish for disease research.
Authors: Matsui, Hideaki and Matsui, Noriko
Journal: Journal of neural transmission (Vienna, Austria : 1996) (2017): 1627-1633
Imaging of compartmentalised intracellular nitric oxide, induced during bacterial phagocytosis, using a metalloprotein-gold nanoparticle conjugate.
Authors: Leggett, Richard and Thomas, Paul and Marín, María J and Gavrilovic, Jelena and Russell, David A
Journal: The Analyst (2017): 4099-4105
Page updated on April 25, 2025

Ordering information

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Catalog Number1720
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Additional ordering information

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Physical properties

Molecular weight

936.19

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.09

Extinction coefficient (cm -1 M -1)

81000

Excitation (nm)

534

Emission (nm)

553

Quantum yield

0.611

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Product Image
Product Image
Gallery Image 1
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a trans‐cyclooctene (TCO)–dye conjugate and a tetrazine‐modified biomolecule. The TCO–dye adduct selectively and rapidly couples with the tetrazine moiety on the biomolecule to form a stable linkage. This bioorthogonal “click chemistry” reaction is fast, selective, and well‐suited for biocompatible labeling applications.