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AAT Bioquest

XFD633 TCO

Product key features

  • Ex/Em: 632/650 nm
  • Extinction Coefficient: 159,000 cm-1M-1
  • Reactive Group: TCO
  • Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules
  • Advanced Dye Properties: Excellent water solubility and structural match to Alexa Fluor™ 633
  • Robust Signal Performance: Maintains high intensity and pH independence (pH 4-11) with exceptional photostability

Product description

XFD633, manufactured by AAT Bioquest, is a bright red-fluorescent dye structurally identical to Alexa Fluor™ 633 (ThermoFisher). It is characterized by its intense brightness, high fluorescence quantum yield and photostability. The dye demonstrates excellent solubility in aqueous solutions and retains pH-independent fluorescence over a broad range (pH 4–11), ensuring consistent performance across diverse experimental conditions. Optimally excited by the 633 nm emission line of He-Ne lasers or the 635 nm diode laser, XFD633 is particularly well-suited for flow cytometry. Its robust and uniform labeling properties yield high signal intensity and reproducibility, making it an ideal choice for advanced fluorescence imaging, flow cytometry, and various fluorescence-based analytical techniques.

XFD633 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Page updated on April 25, 2025

Ordering information

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Catalog Number70077
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.55

Extinction coefficient (cm -1 M -1)

159,000

Excitation (nm)

632

Emission (nm)

650

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a trans‐cyclooctene (TCO)–dye conjugate and a tetrazine‐modified biomolecule. The TCO–dye adduct selectively and rapidly couples with the tetrazine moiety on the biomolecule to form a stable linkage. This bioorthogonal “click chemistry” reaction is fast, selective, and well‐suited for biocompatible labeling applications.
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a trans‐cyclooctene (TCO)–dye conjugate and a tetrazine‐modified biomolecule. The TCO–dye adduct selectively and rapidly couples with the tetrazine moiety on the biomolecule to form a stable linkage. This bioorthogonal “click chemistry” reaction is fast, selective, and well‐suited for biocompatible labeling applications.
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a trans‐cyclooctene (TCO)–dye conjugate and a tetrazine‐modified biomolecule. The TCO–dye adduct selectively and rapidly couples with the tetrazine moiety on the biomolecule to form a stable linkage. This bioorthogonal “click chemistry” reaction is fast, selective, and well‐suited for biocompatible labeling applications.