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AAT Bioquest

XFD532 PEG4 DBCO

Product key features

  • Ex/Em: 534/553 nm
  • Extinction coefficient: 81,000 cm-1M-1
  • Reactive Group: DBCO
  • Copper-Free Bioorthogonal Labeling: Enables rapid and efficient azide conjugation via copper-free SPAAC
  • Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
  • Hydrophilic PEG4 Spacer: Enhances solubility and minimizes steric hindrance for enhanced biocompatibility

Product description

XFD532, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 532 (ThermoFisher). It is a bright yellow-fluorescent dye with an excitation optimized for use with the 532 nm line of the frequency-doubled Nd:YAG laser. The incorporation of a PEG4 linker enhances the aqueous solubility of XFD532, while its pH-insensitivity across a broad pH range (pH 4–10) ensures reliable and stable signal generation under diverse experimental conditions. XFD532 is particularly suited for multicolor fluorescence microscopy and flow cytometry, as well as advanced applications in super-resolution imaging techniques such as dSTORM.

The DBCO derivative of XFD532 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, XFD532 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
XFD488 PEG4 DBCO499520710000.300.11
XFD350 PEG4 DBCO343441190000.250.19
XFD405 PEG4 DBCO40142135,0000.230.70
XFD430 PEG4 DBCO43254015,000-0.28
XFD514 PEG4 DBCO518543800000.310.18
XFD546 PEG4 DBCO5615721120000.210.12
XFD568 PEG4 DBCO579603913000.450.46
XFD594 PEG4 DBCO590618900000.430.56
Page updated on November 9, 2024

Ordering information

Price
Unit size
Catalog Number1719
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

1233.50

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.09

Extinction coefficient (cm -1 M -1)

81000

Excitation (nm)

534

Emission (nm)

553

Quantum yield

0.611

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501