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AAT Bioquest

XFD647 PEG4 DBCO

Product key features

  • Ex/Em: 650/671 nm
  • Extinction coefficient: 270,000 cm-1M-1
  • Reactive Group: DBCO
  • Copper-Free Bioorthogonal Labeling: Enables rapid and efficient azide conjugation via copper-free SPAAC
  • Bright & Stable: Delivers intense fluorescence with resilience to photobleaching and pH variations from 4 to 10
  • Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

Product description

XFD647, manufactured by AAT Bioquest, is a bright far-red flurosecent dye structurally identical to Alexa Fluor™ 647 (ThermoFisher). It is efficiently excited by the 594 nm or 633 nm laser lines and is compatible with RFP filters like Cy5, making it well-suited for applications such as fluorescence microscopy and flow cytometry. XFD647 exhibits excellent aqueous solubility and pH stability across a wide range (pH 4–10), ensuring robust signal reproducibility under diverse experimental conditions. The dye enables high-degree conjugation to biomolecules with minimal self-quenching, resulting in enhanced fluorescence intensity and signal stability. Additionally, its high fluorescence quantum yield and superior photostability facilitate the sensitive detection of low-abundance targets, enhancing precision and sensitivity in quantitative fluorescence-based assays.

The DBCO derivative of XFD647 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, XFD647 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD555 PEG4 DBCO5535681500000.110.080.08
XFD635 PEG4 DBCO633647140,000---
XFD680 PEG4 DBCO6817041840000.3610.000.05
XFD700 PEG4 DBCO6967191920000.2510.000.07
XFD750 PEG4 DBCO7527762400000.1210.000.04
XFD790 PEG4 DBCO782805260,000-0.090.08

References

View all 23 references: Citation Explorer
Key role of cycloalkyne nature in alkyne-dye reagents for enhanced specificity of intracellular imaging by bioorthogonal bioconjugation.
Authors: Vidyakina, Alexandra A and Silonov, Sergey A and Govdi, Anastasia I and Ivanov, Alexander Yu and Podolskaya, Ekaterina P and Balova, Irina A and Bräse, Stefan and Danilkina, Natalia A
Journal: Organic & biomolecular chemistry (2024): 7637-7642
Bridging the Translation of ICG-1-Maltotriose: A Multimodal Sensor for Monitoring and Detecting Bacterial Infections.
Authors: Zlitni, Aimen and Yang, Stella and Achterberg, Friso B and Gowrishankar, Gayatri and Steinberg, Idan and Azevedo, Carmen and Gambhir, Sanjiv S and Valdez, Tulio A
Journal: ACS sensors (2024): 2806-2814
Clickable Albumin Nanoparticles for Pretargeted Drug Delivery toward PD-L1 Overexpressing Tumors in Combination Immunotherapy.
Authors: Gerke, Christoph and Zabala Gutierrez, Irene and Méndez-González, Diego and Cruz, M Carmen Iglesias-de la and Mulero, Francisca and Jaque, Daniel and Rubio-Retama, Jorge
Journal: Bioconjugate chemistry (2022): 821-828
Click-Ready Perfluorocarbon Nanoemulsion for 19F MRI and Multimodal Cellular Detection.
Authors: Perez, Adam S and Zhou, Junhan and Leach, Benjamin and Xu, Hongyan and Lister, Deanne and Adams, Stephen R and Ahrens, Eric T and Louie, Angelique Y
Journal: ACS nanoscience Au (2022): 102-110
Orthogonal Covalent Entrapment of Cargo into Biodegradable Polymeric Micelles via Native Chemical Ligation.
Authors: Hebels, Erik R and Bindt, Felix and Walther, Johanna and van Geijn, Michiel and Weterings, Jimmy and Hu, Qizhi and Colombo, Claudio and Liskamp, Rob and Rijcken, Cristianne and Hennink, Wim E and Vermonden, Tina
Journal: Biomacromolecules (2022)
Page updated on April 25, 2025

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Catalog Number1734
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Physical properties

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.00

Correction Factor (280 nm)

0.03

Extinction coefficient (cm -1 M -1)

239000

Excitation (nm)

650

Emission (nm)

671

Quantum yield

0.331

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Schematic illustrating the strain‐promoted azide–alkyne cycloaddition (SPAAC) between a dibenzocyclooctyne (DBCO)–dye conjugate and an azide‐modified biomolecule. The DBCO’s ring strain drives the copper‐free reaction with the azide to form a stable 1,2,3-triazole linkage, avoiding potential toxicity of copper catalysts. This bioorthogonal labeling strategy proceeds efficiently under mild conditions, making it especially valuable for live‐cell imaging, in vivo studies, and other sensitive bioconjugation applications.
Schematic illustrating the strain‐promoted azide–alkyne cycloaddition (SPAAC) between a dibenzocyclooctyne (DBCO)–dye conjugate and an azide‐modified biomolecule. The DBCO’s ring strain drives the copper‐free reaction with the azide to form a stable 1,2,3-triazole linkage, avoiding potential toxicity of copper catalysts. This bioorthogonal labeling strategy proceeds efficiently under mild conditions, making it especially valuable for live‐cell imaging, in vivo studies, and other sensitive bioconjugation applications.
Schematic illustrating the strain‐promoted azide–alkyne cycloaddition (SPAAC) between a dibenzocyclooctyne (DBCO)–dye conjugate and an azide‐modified biomolecule. The DBCO’s ring strain drives the copper‐free reaction with the azide to form a stable 1,2,3-triazole linkage, avoiding potential toxicity of copper catalysts. This bioorthogonal labeling strategy proceeds efficiently under mild conditions, making it especially valuable for live‐cell imaging, in vivo studies, and other sensitive bioconjugation applications.