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AAT Bioquest

XFD680 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD680 Same Structure to Alexa Fluor™ 680*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (280 nm)0.05
Extinction coefficient (cm -1 M -1)183000
Excitation (nm)681
Emission (nm)704
Quantum yield0.361
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
Host
goat
Reactivity
human
Application
Flow Cytometry (FACS); ELISA; IHC; Western Blot
Correction Factor (280 nm)
0.05
Extinction coefficient (cm -1 M -1)
183000
Excitation (nm)
681
Emission (nm)
704
Quantum yield
0.361
AAT Bioquest's anti-human secondary antibodies have well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. This Alexa Fluor® 680-labeled secondary antibody was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar Alexa Fluor® 680 goat anti-human IgG antibodies from other commercial sources, and thus can significantly increase assay sensitivities. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. This antibody was purified through affinity chromatography and conjugated to XFD680 (ex/em = 681/704 nm). XFD680 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 680 (Alexa Fluor® is the trademark of ThermoFisher).

Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.05
Extinction coefficient (cm -1 M -1)183000
Excitation (nm)681
Emission (nm)704
Quantum yield0.361

Conjugation


We provide custom conjugation services for this antibody (eg. labeling of IgG antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.